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Function of the Speech Recognition of the Smartphone to Automatically Operate a Portable Sample Pretreatment Microfluidic System

  • Hoang Khang Bui
    Hoang Khang Bui
    Department of Chemical Engineering (BK21 FOUR Integrated Engineering Program), Kyung Hee University, Yongin, 17104, South Korea
  • Vu Minh Phan
    Vu Minh Phan
    Department of Chemical Engineering (BK21 FOUR Integrated Engineering Program), Kyung Hee University, Yongin, 17104, South Korea
    More by Vu Minh Phan
  • Huynh Quoc Nguyen
    Huynh Quoc Nguyen
    Department of Chemical Engineering (BK21 FOUR Integrated Engineering Program), Kyung Hee University, Yongin, 17104, South Korea
  • Van Dan Nguyen
    Van Dan Nguyen
    Department of Chemical Engineering (BK21 FOUR Integrated Engineering Program), Kyung Hee University, Yongin, 17104, South Korea
  • Hiep Van Nguyen
    Hiep Van Nguyen
    Department of Chemical Engineering (BK21 FOUR Integrated Engineering Program), Kyung Hee University, Yongin, 17104, South Korea
  • , and 
  • Tae Seok Seo*
    Tae Seok Seo
    Department of Chemical Engineering (BK21 FOUR Integrated Engineering Program), Kyung Hee University, Yongin, 17104, South Korea
    *Email: [email protected]
    More by Tae Seok Seo
Cite this: ACS Sens. 2023, 8, 2, 515–521
Publication Date (Web):February 1, 2023
https://doi.org/10.1021/acssensors.2c01849
Copyright © 2023 American Chemical Society
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Abstract

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We proposed a portable sample pretreatment microsystem, which can be automatically operated through speech recognition in a smartphone app. The proposed sample pretreatment microsystem consists of a microfluidic chip, an air router, pressure and vacuum lines with air pump motors, six 3-way solenoid valves, and a microcontroller with a Bluetooth module. The command of a human voice conducted the whole process of DNA extraction from pathogenic bacterial samples. Thus, manual interference during the DNA extraction is eliminated, preventing any potential infection from human touch. The palm-sized sample pretreatment microsystem can be run by a portable battery or a conventional smartphone charger. Genomic DNA ofSalmonella typhimuriumwas purified on a chip in less than 1 min with an extraction efficiency of 70 ± 5%.

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The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acssensors.2c01849.

  • List for the materials and cost; MIT App Inventor code block; PCB design and its real image; primer sequences for qPCR (PDF)

  • Movie 1: Demonstration of the diversity in voice commands in the speech recognition system (MP4)

  • Movie 2: Demonstration of the voice-controlled operation for the sample pretreatment (MP4)

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Most electronic Supporting Information files are available without a subscription to ACS Web Editions. Such files may be downloaded by article for research use (if there is a public use license linked to the relevant article, that license may permit other uses). Permission may be obtained from ACS for other uses through requests via the RightsLink permission system: http://pubs.acs.org/page/copyright/permissions.html.

Cited By

This article is cited by 1 publications.

  1. Michael J. Sailor. The Three Laws of Nano-Robotics. ACS Sensors 2023, 8 (5) , 1868-1870. https://doi.org/10.1021/acssensors.3c00920

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