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Temperature-Robust DNAzyme Biosensors Confirming Ultralow Background Detection

  • Ruijie Deng
    Ruijie Deng
    College of Light Industry, Textile and Food Engineering, Healthy Food Evaluation Research Center and Key Laboratory of Food Science and Technology of Ministry of Education of Sichuan Province, Sichuan University, Chengdu 610065, China
    Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing 100084, China
    More by Ruijie Deng
  • Hao Yang
    Hao Yang
    College of Light Industry, Textile and Food Engineering, Healthy Food Evaluation Research Center and Key Laboratory of Food Science and Technology of Ministry of Education of Sichuan Province, Sichuan University, Chengdu 610065, China
    More by Hao Yang
  • Yi Dong
    Yi Dong
    College of Light Industry, Textile and Food Engineering, Healthy Food Evaluation Research Center and Key Laboratory of Food Science and Technology of Ministry of Education of Sichuan Province, Sichuan University, Chengdu 610065, China
    More by Yi Dong
  • Zhifeng Zhao*
    Zhifeng Zhao
    College of Light Industry, Textile and Food Engineering, Healthy Food Evaluation Research Center and Key Laboratory of Food Science and Technology of Ministry of Education of Sichuan Province, Sichuan University, Chengdu 610065, China
    *E-mail:[email protected].
    More by Zhifeng Zhao
  • Xuhan Xia
    Xuhan Xia
    College of Light Industry, Textile and Food Engineering, Healthy Food Evaluation Research Center and Key Laboratory of Food Science and Technology of Ministry of Education of Sichuan Province, Sichuan University, Chengdu 610065, China
    More by Xuhan Xia
  • Yue Li
    Yue Li
    Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing 100084, China
    More by Yue Li
  • Jinghong Li*
    Jinghong Li
    Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing 100084, China
    *E-mail: [email protected].
    More by Jinghong Li
Cite this: ACS Sens. 2018, 3, 12, 2660-2666
Publication Date (Web):November 20, 2018
https://doi.org/10.1021/acssensors.8b01122
Copyright © 2018 American Chemical Society
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Abstract

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Catalytic DNA/RNA, such as DNAzyme, has been widely adopted to construct biosensors, especially for metal ion analysis. However, traditional DNAzyme biosensors still suffer from fluctuating and relatively high background. Herein, we proposed a temperature-robust DNAzyme, conferring ultralow background in various temperatures, thus leading to highly sensitive and robust detection of metal ions. Instead of labeling substrate to directly output fluorescence signal, our proposed DNAzyme biosensor utilized a sequential detection process with a couple of proximity fluorescent probes, confirming very low background regardless of the conditions of cleavage reaction. This sequential DNAzyme biosensor conferred a signal to background ratio over 20 when the temperature of the catalytic reaction ranged from 20 to 41 °C. Benefitting from its ultralow background, it could confer a detection limit of 0.22 nM, which ranked as one of the highest sensitivity levels among DNAzyme-based fluorescent biosensors. This DNAzyme biosensor was over 6000 times more selective for Pb2+ against the most active interfering metal ions, Zn2+. Further, it has been successfully applied for analyzing lead pollution in tap water and eggs, with total recoveries ranging from 87% to 114%. This facile, simple, and effective design strategy would significantly improve the detection performance of DNAzyme biosensors, thus facilitating its practical applications for both food safety analysis and environment monitoring.

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The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acssensors.8b01122.

  • Kinetics of sequential reaction process, secondary structures of DNAzyme–substrate hybridization, quantification performance of single-labeled and dual-labeled DNAzyme. Recovery of Pb2+ in the tap water and egg samples. (PDF)

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Cited By


This article is cited by 3 publications.

  1. Longlong Gao, Xingyu Tong, Ting Ye, Heng Gao, Qingqing Zhang, Chenxiao Yan, Yali Yu, Yifan Fei, Xiaoshun Zhou, Yong Shao. G‐Quadruplex‐Based Photooxidase Driven by Visible Light. ChemCatChem 2020, 12 (1) , 169-174. DOI: 10.1002/cctc.201901481.
  2. Zhifeng Zhao, Hao Yang, Sha Deng, Yi Dong, Bin Yan, Kaixiang Zhang, Ruijie Deng, Qiang He. Intrinsic conformation response-leveraged aptamer probe based on aggregation-induced emission dyes for aflatoxin B1 detection. Dyes and Pigments 2019, 171, 107767. DOI: 10.1016/j.dyepig.2019.107767.
  3. Fengjie Yang, Yuan Liu, Bin Wang, Changjun Zhou, Qiang Zhang. Constructing Controllable Logic Circuits Based on DNAzyme Activity. Molecules 2019, 24 (22) , 4134. DOI: 10.3390/molecules24224134.

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