ACS Publications. Most Trusted. Most Cited. Most Read

OR SEARCH CITATIONS

My Activity
Publications
CONTENT TYPES

Figure 1Loading Img
Download Hi-Res ImageDownload to MS-PowerPointCite This:ACS Sens. 2019, 4, 5, 1346-1357
RETURN TO ISSUEPREVArticleNEXT

Label-Free Optical Detection of Multiple Biomarkers in Sweat, Plasma, Urine, and Saliva

Cite this: ACS Sens. 2019, 4, 5, 1346–1357
Publication Date (Web):March 22, 2019
https://doi.org/10.1021/acssensors.9b00301
Copyright © 2019 American Chemical Society
Request reuse permissions

    Article Views

    4596

    Altmetric

    -

    Citations

    53
    LEARN ABOUT THESE METRICS
    Read OnlinePDF (6 MB)
    Get e-Alerts
    Supporting Info (1)»
    SUBJECTS:

    Abstract

    Abstract Image

    We report a novel label-free quantitative detection of human performance “stress” biomarkers in different body fluids based on optical absorbance of the biomarkers in the ultraviolet (UV) region. Stress biomarker (hormones and neurotransmitters) concentrations in bodily fluids (blood, sweat, urine, saliva) predict the physical and mental state of the individual. The stress biomarkers primarily focused on in this manuscript are cortisol, serotonin, dopamine, norepinephrine, and neuropeptide Y. UV spectroscopy of stress biomarkers performed in the 190–400 nm range has revealed primary and secondary absorption peaks at near-UV wavelengths depending on their molecular structure. UV characterization of individual and multiple biomarkers is reported in various biofluids. A microfluidic/optoelectronic platform for biomarker detection is reported, with a prime focus toward cortisol evaluation. The current limit of detection of cortisol in sweat is ∼200 ng/mL (∼0.5 μM), which is in the normal (healthy) range. Plasma samples containing both serotonin and cortisol resulted in readily detectable absorption peaks at 203 (serotonin) and 247 (cortisol) nm, confirming feasibility of simultaneous detection of multiple biomarkers in biofluid samples. UV spectroscopy performed on various stress biomarkers shows a similar increasing absorption trend with concentration. The detection mechanism is label free, applicable to a variety of biomarker types, and able to detect multiple biomarkers simultaneously in various biofluids. A microfluidic flow cell has been fabricated on a polymer substrate to enable point-of-use/care UV measurement of target biomarkers. The overall sensor combines sample dispensing and fluid transport to the detection location with optical absorption measurements with a UV light emitting diode (LED) and photodiode. The biomarker concentration is indicated as a function of photocurrent generated at the target wavelength.

    KEYWORDS:

    Supporting Information

    ARTICLE SECTIONS
    Jump To

    The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acssensors.9b00301.

    • Spectral characterization (including signal deconvolution) in plasma, urine, and saliva for cortisol and serotonin; UV LED/PD characteristics (PDF)

    Terms & Conditions

    Most electronic Supporting Information files are available without a subscription to ACS Web Editions. Such files may be downloaded by article for research use (if there is a public use license linked to the relevant article, that license may permit other uses). Permission may be obtained from ACS for other uses through requests via the RightsLink permission system: http://pubs.acs.org/page/copyright/permissions.html.

    Cited By

    This article is cited by 53 publications.

    1. Xun Liu, Naikun Song, Dahong Qian, Sai Gu, Jun Pu, Lin Huang, Jian Liu, Kun Qian. Porous Inorganic Materials for Bioanalysis and Diagnostic Applications. ACS Biomaterials Science & Engineering 2022, 8 (10) , 4092-4109. https://doi.org/10.1021/acsbiomaterials.1c00733
    2. Jing Zhao, Siyue Zhang, Ye Sun, Ning Zhou, Hui Yu, Hongxia Zhang, Dagong Jia. Wearable Optical Sensing in the Medical Internet of Things (MIoT) for Pervasive Medicine: Opportunities and Challenges. ACS Photonics 2022, 9 (8) , 2579-2599. https://doi.org/10.1021/acsphotonics.2c00898
    3. Shima Dalirirad, Daewoo Han, Andrew J. Steckl. Aptamer-Based Lateral Flow Biosensor for Rapid Detection of Salivary Cortisol. ACS Omega 2020, 5 (51) , 32890-32898. https://doi.org/10.1021/acsomega.0c03223
    4. Somayeh Jafarinejad, Arafeh Bigdeli, Mahmoud Ghazi-Khansari, Pezhman Sasanpour, M. Reza Hormozi-Nezhad. Identification of Catecholamine Neurotransmitters Using a Fluorescent Electronic Tongue. ACS Chemical Neuroscience 2020, 11 (1) , 25-33. https://doi.org/10.1021/acschemneuro.9b00537
    5. Mengyuan Song, Hao Bai, Ping Zhang, Xuedong Zhou, Binwu Ying. Promising applications of human-derived saliva biomarker testing in clinical diagnostics. International Journal of Oral Science 2023, 15 (1) https://doi.org/10.1038/s41368-022-00209-w
    6. M. Hassani-Marand, S. Jafarinejad, M.R. Hormozi-Nezhad. An AI-enabled multi colorimetric sensor array: Towards rapid and noninvasive detection of neuroblastoma urinary markers. Sensors and Actuators B: Chemical 2023, 396 , 134571. https://doi.org/10.1016/j.snb.2023.134571
    7. Yu Jin Chi, Byeongseok Ryu, Sujeong Ahn, Won-Gun Koh. A colorimetric biosensor based on a biodegradable fluidic device capable of efficient saliva sampling and salivary biomarker detection. Sensors and Actuators B: Chemical 2023, 396 , 134601. https://doi.org/10.1016/j.snb.2023.134601
    8. Biddut K. Sarker, Cheri M. Hampton, Lawrence F. Drummy. Graphene Field‐Effect Transistors for Sensing Stress and Fatigue Biomarkers. 2023, 339-372. https://doi.org/10.1002/9783527843374.ch17
    9. Chensong Xu, Gwenaël Bonfante, Jongho Park, Vincent Salles, Beomjoon Kim. Fabrication of an electrospun polycaprolactone substrate for colorimetric bioassays. Biomedical Microdevices 2023, 25 (3) https://doi.org/10.1007/s10544-023-00673-z
    10. Andreja Abina, Tjaša Korošec, Uroš Puc, Mojca Jazbinšek, Aleksander Zidanšek. Urinary Metabolic Biomarker Profiling for Cancer Diagnosis by Terahertz Spectroscopy: Review and Perspective. Photonics 2023, 10 (9) , 1051. https://doi.org/10.3390/photonics10091051
    11. Zina Fredj, Pengbo Wang, Fateh Ullah, Mohamad Sawan. A nanoplatform-based aptasensor to electrochemically detect epinephrine produced by living cells. Microchimica Acta 2023, 190 (9) https://doi.org/10.1007/s00604-023-05902-z
    12. Andri Hardiansyah, Gardin Muhammad Andika Saputra, Hikmat Hikmat, Yuniar Elfira Kusfarida, Ni Luh Wulan Septiani, Ahmad Randy, Angga Hermawan, Brian Yuliarto, Ting‐Yu Liu, Tetsuya Kida. Electrochemical evaluation of magnetic reduced graphene oxide nanosheet‐modified glassy carbon electrode on dopamine electrochemical sensor for Parkinson's diagnostic application. Journal of the Chinese Chemical Society 2023, 70 (8) , 1665-1682. https://doi.org/10.1002/jccs.202300197
    13. I. Bányász, I. Rajta, V. Havránek, A. Mackova, A. J. Laki, M. S. Z. Kellermayer, Z. Szittner, S. Kurunczi, Sz. Novák, I. Székács, R. Horváth, M. Fried, G. U. L. Nagy. Design, fabrication, and characterization of picowell arrays on cyclic olefin copolymer surfaces generated with a 10.5 MeV N4+ ion microbeam. Applied Physics Letters 2023, 123 (5) https://doi.org/10.1063/5.0155681
    14. Pushan Guha Roy, Sayantani Sen, Anirban Bhattacharyya. Wavelength-switchable ultraviolet light-emitting diodes. Optics Letters 2023, 48 (11) , 3099. https://doi.org/10.1364/OL.490036
    15. Dang Du Nguyen, Seho Lee, Inki Kim. Recent Advances in Metaphotonic Biosensors. Biosensors 2023, 13 (6) , 631. https://doi.org/10.3390/bios13060631
    16. Jingjing Li, Sang Hyuk Lee, Dong Kyu Yoo, Ho Chul Woo, Sung Hwa Jhung, Milica Jović, Hubert Girault, Hye Jin Lee. A spatially multiplexed voltammetric magneto-sandwich assay involving Fe3O4/Fe-based metal-organic framework for dual liver cancer biomarkers. Sensors and Actuators B: Chemical 2023, 380 , 133313. https://doi.org/10.1016/j.snb.2023.133313
    17. Ayman Negm, Matiar M.R. Howlader, Mohamed Bakr, Shirook Ali. Biomarker detection using GST-based permittivity-asymmetric metasurface. Materials & Design 2023, 227 , 111747. https://doi.org/10.1016/j.matdes.2023.111747
    18. Yanke Zhang, Qingteng Lai, Wei Chen, Chi Zhang, Long Mo, Zhengchun Liu. Recent Advance in Cortisol Immunosensing Technologies and Devices. Chemosensors 2023, 11 (2) , 90. https://doi.org/10.3390/chemosensors11020090
    19. Xin Zhang, Zhiheng Zhang, Weize Diao, Chuangxin Zhou, Yetong Song, Renzhi Wang, Xiaoguang Luo, Guozhen Liu. Early-diagnosis of major depressive disorder: From biomarkers to point-of-care testing. TrAC Trends in Analytical Chemistry 2023, 159 , 116904. https://doi.org/10.1016/j.trac.2022.116904
    20. Annalisa Scroccarello, Flavio Della Pelle, Michele Del Carlo, Dario Compagnone. Optical plasmonic sensing based on nanomaterials integrated in solid supports. A critical review. Analytica Chimica Acta 2023, 1237 , 340594. https://doi.org/10.1016/j.aca.2022.340594
    21. Michael Breitenbach, Elisabeth Kapferer, Clemens Sedmak. Wie misst man Stress?. 2023, 109-130. https://doi.org/10.1007/978-3-031-23697-6_6
    22. Mahdi Bahadoran, Abbas Kalate Seyfari, Parisa Sanati, Lee Suan Chua. Label free identification of the different status of anemia disease using optimized double-slot cascaded microring resonator. Scientific Reports 2022, 12 (1) https://doi.org/10.1038/s41598-022-09504-2
    23. Parijat Deshpande, Bharath Ravikumar, Siddharth Tallur, Debjani Paul, Beena Rai. Development of an insilico model of eccrine sweat using molecular modelling techniques. Scientific Reports 2022, 12 (1) https://doi.org/10.1038/s41598-022-24440-x
    24. Hui ZHENG, MengYuan LIU, XianQing YANG, Yun CAO, WeiRong NIE, ShuQi WANG, Ting ZHANG. Preparation of the artificial “skin” uniform sweating simulation device. SCIENTIA SINICA Technologica 2022, 52 (11) , 1769-1776. https://doi.org/10.1360/SST-2022-0114
    25. Nuria Borras, Margarita Sánchez‐Jiménez, Jordi Casanovas, Carlos Alemán, Maria M. Pérez‐Madrigal. Porous Poly(3,4‐ethylenedioxythiophene)‐Based Electrodes for Detecting Stress Biomarkers in Artificial Urine and Sweat. Macromolecular Materials and Engineering 2022, 307 (10) https://doi.org/10.1002/mame.202200269
    26. Kazuma Hashimoto, Paul Ben Ishai, Erik Bründermann, Saroj R. Tripathi. Dielectric property measurement of human sweat using attenuated total reflection terahertz time domain spectroscopy. Biomedical Optics Express 2022, 13 (9) , 4572. https://doi.org/10.1364/BOE.467450
    27. Aiman Tanveer, Dinesh Yadav. Omics for Biomarker Investigation in Neurodegenerative Diseases. 2022, 143-160. https://doi.org/10.2174/9789815040913122010012
    28. Maria João Nunes, José J. G. Moura, João Paulo Noronha, Luís Cobra Branco, Alejandro Samhan-Arias, João P. Sousa, Carlos Rouco, Cristina M. Cordas. Evaluation of Sweat-Sampling Procedures for Human Stress-Biomarker Detection. Analytica 2022, 3 (2) , 178-194. https://doi.org/10.3390/analytica3020013
    29. Sanjida Yeasmin, Bo Wu, Ye Liu, Ahasan Ullah, Li-Jing Cheng. Nano gold-doped molecularly imprinted electrochemical sensor for rapid and ultrasensitive cortisol detection. Biosensors and Bioelectronics 2022, 206 , 114142. https://doi.org/10.1016/j.bios.2022.114142
    30. Tatiana S. Ponomaryova, Anastasiya S. Novikova, Daniil D. Drozd, Pavel D. Strokin, Olga A. Goryacheva, Artem A. Bakal, Irina Y. Goryacheva, , . Evaluation of the influence of the matrix effects of blood and serum on the optical properties of luminescent quantum dots. 2022, 22. https://doi.org/10.1117/12.2626115
    31. Juan Wang, Stefan A. Maier, Andreas Tittl. Trends in Nanophotonics‐Enabled Optofluidic Biosensors. Advanced Optical Materials 2022, 10 (7) https://doi.org/10.1002/adom.202102366
    32. Nandini Swaminathan, Nallin Sharma, Hui-Fen Wu. Impact of ascorbic acid on polydopamine modified amorphous TiO2-xNx nanosheets. Applied Surface Science 2022, 576 , 151758. https://doi.org/10.1016/j.apsusc.2021.151758
    33. Fatemeh Haghayegh, Razieh Salahandish, Azam Zare, Mahmood Khalghollah, Amir Sanati-Nezhad. Immuno-biosensor on a chip: a self-powered microfluidic-based electrochemical biosensing platform for point-of-care quantification of proteins. Lab on a Chip 2021, 22 (1) , 108-120. https://doi.org/10.1039/D1LC00879J
    34. Md. Azahar Ali, Chunshan Hu, Bin Yuan, Sanjida Jahan, Mohammad S. Saleh, Zhitao Guo, Andrew J. Gellman, Rahul Panat. Breaking the barrier to biomolecule limit-of-detection via 3D printed multi-length-scale graphene-coated electrodes. Nature Communications 2021, 12 (1) https://doi.org/10.1038/s41467-021-27361-x
    35. Kazuma Hashimoto, Hikaru Sakata, Saroj R. Tripathi. Measurement of dielectric properties of human sweat in terahertz frequency region. 2021, 1-2. https://doi.org/10.1109/IRMMW-THz50926.2021.9567344
    36. Meng-Hua Tang, Ying Shi, Xiao-Lei Jiang, Hang Xu, Yue Ma, Bin Zhao. A high sensitivity luminescent sensor for the stress biomarker cortisol using four-fold interpenetrated europium–organic frameworks integrated with logic gates. Journal of Materials Chemistry C 2021, 9 (30) , 9643-9649. https://doi.org/10.1039/D1TC01342D
    37. Jijo Lukose, Sanoop Pavithran M., Mithun N., Ajaya Kumar Barik, Keerthilatha M. Pai, V. K. Unnikrishnan, Sajan D. George, V. B. Kartha, Santhosh Chidangil. Photonics of human saliva: potential optical methods for the screening of abnormal health conditions and infections. Biophysical Reviews 2021, 13 (3) , 359-385. https://doi.org/10.1007/s12551-021-00807-8
    38. Rasha Rahman Poolakkandy, Mini Mol Menamparambath. Transition metal oxide based non‐enzymatic electrochemical sensors: An arising approach for the meticulous detection of neurotransmitter biomarkers. Electrochemical Science Advances 2021, 1 (2) https://doi.org/10.1002/elsa.202000024
    39. Cátia Leitão, Arnaldo Leal-Junior, Ana R. Almeida, Sónia O. Pereira, Florinda M. Costa, João L. Pinto, C. Marques. Cortisol AuPd plasmonic unclad POF biosensor. Biotechnology Reports 2021, 29 , e00587. https://doi.org/10.1016/j.btre.2021.e00587
    40. Michael Breitenbach, Elisabeth Kapferer, Clemens Sedmak. Measuring Stress. 2021, 95-113. https://doi.org/10.1007/978-3-030-77738-8_6
    41. Tejaswini Appidi, Sushma V. Mudigunda, Suseela Kodandapani, Aravind Kumar Rengan. Development of label-free gold nanoparticle based rapid colorimetric assay for clinical/point-of-care screening of cervical cancer. Nanoscale Advances 2020, 2 (12) , 5737-5745. https://doi.org/10.1039/D0NA00686F
    42. Van Long Huynh, Tran Quang Trung, Montri Meeseepong, Han‐Byeol Lee, Trong Danh Nguyen, Nae‐Eung Lee. Hollow Microfibers of Elastomeric Nanocomposites for Fully Stretchable and Highly Sensitive Microfluidic Immunobiosensor Patch. Advanced Functional Materials 2020, 30 (46) https://doi.org/10.1002/adfm.202004684
    43. Mani Arivazhagan, Govindhan Maduraiveeran. Ultra-fine nickel sulfide nanoclusters @ nickel sulfide microsphere as enzyme-free electrode materials for sensitive detection of lactic acid. Journal of Electroanalytical Chemistry 2020, 874 , 114465. https://doi.org/10.1016/j.jelechem.2020.114465
    44. Hiroki Hayashi, Naoki Sakamoto, Sho Hideshima, Yoshitaka Harada, Mika Tsuna, Shigeki Kuroiwa, Keishi Ohashi, Toshiyuki Momma, Tetsuya Osaka. Tetrameric jacalin as a receptor for field effect transistor biosensor to detect secretory IgA in human sweat. Journal of Electroanalytical Chemistry 2020, 873 , 114371. https://doi.org/10.1016/j.jelechem.2020.114371
    45. Daewoo Han, Prajokta Ray, Shima Dalirirad, Andrew Steckl. Novel point of care strategies for biomarker detection. 2020, 1-2. https://doi.org/10.1109/RAPID49481.2020.9195654
    46. Rares Drula, Leonie Florence Ott, Ioana Berindan-Neagoe, Klaus Pantel, George A. Calin. MicroRNAs from Liquid Biopsy Derived Extracellular Vesicles: Recent Advances in Detection and Characterization Methods. Cancers 2020, 12 (8) , 2009. https://doi.org/10.3390/cancers12082009
    47. Timothy Shay, Tamoghna Saha, Michael D. Dickey, Orlin D. Velev. Principles of long-term fluids handling in paper-based wearables with capillary–evaporative transport. Biomicrofluidics 2020, 14 (3) https://doi.org/10.1063/5.0010417
    48. Ankit Kumar Pandey, Anuj K. Sharma, Carlos Marques. On The Application of SiO2/SiC Grating on Ag for High-Performance Fiber Optic Plasmonic Sensing of Cortisol Concentration. Materials 2020, 13 (7) , 1623. https://doi.org/10.3390/ma13071623
    49. Kyung Ho Kim, Sang Hun Lee, Sung Eun Seo, Joonwon Bae, Seon Joo Park, Oh Seok Kwon. Ultrasensitive Stress Biomarker Detection Using Polypyrrole Nanotube Coupled to a Field-Effect Transistor. Micromachines 2020, 11 (4) , 439. https://doi.org/10.3390/mi11040439
    50. Sajal Shrivastava, Tran Quang Trung, Nae-Eung Lee. Recent progress, challenges, and prospects of fully integrated mobile and wearable point-of-care testing systems for self-testing. Chemical Society Reviews 2020, 49 (6) , 1812-1866. https://doi.org/10.1039/C9CS00319C
    51. Alice Bartolozzi, Federica Viti, Silvia De Stefano, Francesca Sbrana, Loredana Petecchia, Paola Gavazzo, Massimo Vassalli. Development of label-free biophysical markers in osteogenic maturation. Journal of the Mechanical Behavior of Biomedical Materials 2020, 103 , 103581. https://doi.org/10.1016/j.jmbbm.2019.103581
    52. Ying Zhang, Wang Ren, Yu Zhu Fan, Hong Qun Luo, Nian Bing Li. Chemically-modulated turn-on fluorescence for rapid and visual discrimination of norepinephrine and epinephrine and its application for dopamine-β-hydroxylase detection. Sensors and Actuators B: Chemical 2020, 305 , 127463. https://doi.org/10.1016/j.snb.2019.127463
    53. Junning Qian, Hui Xu. Insight into optical properties of carbazole-supported on CeO 2 /TiO 2 composite. Materials Research Express 2019, 6 (11) , 116206. https://doi.org/10.1088/2053-1591/ab47cb
    Download PDF

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    You’ve supercharged your research process with ACS and Mendeley!

    STEP 1:
    Click to create an ACS ID

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    MENDELEY PAIRING EXPIRED
    Your Mendeley pairing has expired. Please reconnect