logo
My Activity
Recently Viewed
You have not visited any articles yet, Please visit some articles to see contents here.
CONTENT TYPES

A Microfluidic Sensor for Continuous, in Situ Surface Charge Measurement of Single Cells

  • Liwei Ni
    Liwei Ni
    Department of Mechanical Engineering, University of Akron, Akron, Ohio 44325, United States
    More by Liwei Ni
  • Rubia Shaik
    Rubia Shaik
    Department of Biomedical Engineering, University of Akron, Akron, Ohio 44325, United States
    More by Rubia Shaik
  • Ruiting Xu
    Ruiting Xu
    Department of Mechanical Engineering, University of Akron, Akron, Ohio 44325, United States
    More by Ruiting Xu
  • Ge Zhang*
    Ge Zhang
    Department of Biomedical Engineering, University of Akron, Akron, Ohio 44325, United States
    *E-mail: [email protected] (G.Z.).
    More by Ge Zhang
  • Jiang Zhe*
    Jiang Zhe
    Department of Mechanical Engineering, University of Akron, Akron, Ohio 44325, United States
    *E-mail: [email protected] (J.Z.).
    More by Jiang Zhe
Cite this: ACS Sens. 2020, XXXX, XXX, XXX-XXX
Publication Date (Web):January 15, 2020
https://doi.org/10.1021/acssensors.9b02411
Copyright © 2020 American Chemical Society
Article Views
108
Altmetric
-
Citations
-
LEARN ABOUT THESE METRICS

Article Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.

Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.

The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated.

Read OnlinePDF (2 MB)
Supporting Info (1)»

Abstract

Abstract Image

Cell surface charge has been recognized as an important cellular property. We developed a microfluidic sensor based on resistive pulse sensing to assess surface charge and sizes of single cells suspended in a continuous flow. The device consists of two consecutive resistive pulse sensors (RPSs) with identical dimensions. Opposite electric fields were applied on the two RPSs. A charged cell in the RPSs was accelerated or decelerated by the electric fields and thus exhibited different transit times passing through the two RPSs. The cell surface charge is measured with zeta potential that can be quantified with the transit time difference. The transit time of each cell can be accurately detected with the width of pulses generated by the RPS, while the cell size can be calculated with the pulse magnitude at the same time. This device has the ability to detect surface charges and sizes of individual cells with high tolerance in cell types and testing solutions compared with traditional electrophoretic light scattering methods. Three different types of cells including HeLa cancer cells, human dermal fibroblast cells, and human umbilical vein endothelial cells (HUVECs) were tested with the sensor. Results showed a significant difference of zeta potentials between HeLa cells and fibroblasts or HUVECs. In addition, when HeLa cells were treated with various concentrations of glutamine, the effects on cancer cell surface charge were detected. Our results demonstrated the great potential of using our sensor for cell type sorting, cancer cell detection, and cell status analysis.

Supporting Information

ARTICLE SECTIONS
Jump To

The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acssensors.9b02411.

  • Supporting figures of electric field modeling and particle/cell size distribution (PDF)

Terms & Conditions

Electronic Supporting Information files are available without a subscription to ACS Web Editions. The American Chemical Society holds a copyright ownership interest in any copyrightable Supporting Information. Files available from the ACS website may be downloaded for personal use only. Users are not otherwise permitted to reproduce, republish, redistribute, or sell any Supporting Information from the ACS website, either in whole or in part, in either machine-readable form or any other form without permission from the American Chemical Society. For permission to reproduce, republish and redistribute this material, requesters must process their own requests via the RightsLink permission system. Information about how to use the RightsLink permission system can be found at http://pubs.acs.org/page/copyright/permissions.html.

Cited By


This article has not yet been cited by other publications.

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    You’ve supercharged your research process with ACS and Mendeley!

    STEP 1:
    Click to create an ACS ID

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    OOPS

    You have to login with your ACS ID befor you can login with your Mendeley account.

    MENDELEY PAIRING EXPIRED
    Your Mendeley pairing has expired. Please reconnect

    This website uses cookies to improve your user experience. By continuing to use the site, you are accepting our use of cookies. Read the ACS privacy policy.

    CONTINUE