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Silane Grafted Biosourced Melanin: A Sustainable Approach for Nanobiosensing Applications
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    Research Article

    Silane Grafted Biosourced Melanin: A Sustainable Approach for Nanobiosensing Applications
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    • Mariana Caldas
      Mariana Caldas
      3B’s Research Group, I3Bs − Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, Barco, 4805-017 Guimarães, Portugal
    • Miguel Neves
      Miguel Neves
      3B’s Research Group, I3Bs − Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, Barco, 4805-017 Guimarães, Portugal
      More by Miguel Neves
    • Albina R. Franco
      Albina R. Franco
      3B’s Research Group, I3Bs − Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, Barco, 4805-017 Guimarães, Portugal
    • Mrinal Bhattacharya
      Mrinal Bhattacharya
      Department of Biosystems Engineering, University of Minnesota, St. Paul, Minnesota 55108, United States
    • Rui L. Reis
      Rui L. Reis
      3B’s Research Group, I3Bs − Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, Barco, 4805-017 Guimarães, Portugal
      More by Rui L. Reis
    • Vitor M. Correlo*
      Vitor M. Correlo
      3B’s Research Group, I3Bs − Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, Barco, 4805-017 Guimarães, Portugal
      *Telephone: +351-253-51093. E-mail: [email protected]
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    ACS Sustainable Chemistry & Engineering

    Cite this: ACS Sustainable Chem. Eng. 2024, 12, 6, 2149–2161
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    https://doi.org/10.1021/acssuschemeng.3c04911
    Published January 31, 2024
    Copyright © 2024 American Chemical Society

    Abstract

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    Nanotechnology has revolutionized areas of biotechnology owing to its multidiversified applications, improved sensitivity, surface size, and cost. In the past years, some issues have arisen regarding the impact of nanomaterials on the environment and human health. In this regard, there is a growing interest in identifying natural and sustainable nanostructured biomaterials as an alternative to toxic petrochemical derived materials. The most commonly used materials in nanobiotechnology applications possess inherent limitations regarding biocompatibility, which leads to foreign-body response at the implantation site, causing a rejection of the implant. In parallel, the recovery of byproducts from other industries and their transformation into valuable resources have been key factors in the circular economy. Given that, there is urgency to move toward sustainable research and green nanotechnologies with an undeniable biocompatibility. Herein, we propose a design based on natural melanin nanoparticles (MNPs) obtained from the ink sacs of Sepia officinalis, a waste product from the food industry, grafted with silane (MNPs GPTMS) for biomedical applications. The design, characterization of both physical and chemical properties, and biological properties of the MNPs GPTMS are reported. The silane, (3-glycidyloxypropyl)trimethoxysilane, allows facile functionalization of different bioreceptors onto the surface of MNPs GPTMS, herein demonstrated with antibodies and an enzyme. Cellular viability studies confirmed the cytocompatibility of MNPs GPTMS and hemocompatibility of 50 μg/mL. The engineered MNPs GPTMS provide a powerful, sustainable, and biocompatible biomaterial-based tool for specific bioreceptor targeting molecules that can be used in diverse biomedical field applications such as nanobiosensing.

    Copyright © 2024 American Chemical Society

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    Supporting Information

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    The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acssuschemeng.3c04911.

    • AFM section analysis of MNPs; AFM topographical image of MNPs and MNPs GPTMS; size of MNPs GPTMS dispersed in water, PBS or DMEM; FTIR spectra of MNPs, MNPs GPTMS and MNPs GPTMS coupled with different antibodies; XPS analysis of C 1s core-level spectrum of MNPs, MNPs GPTMS and MNPs GPTMS Ab; XPS analysis of oxygen (1s) of MNPs, MNPs GPTMS and MNPs GPTMS Ab; microscopic images of live and dead SaOs-2 cells after 24 h incubation with MNPs GPTMS; microscopic images of live and dead L929 cells after 24 h incubation with MNPs GPTMS; photothermal effect of MNPs and MNPs GPTMS; components of O 1s peak and respective peak energies of MNPs and MNPs GPTMS (PDF)

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    Cited By

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    This article is cited by 2 publications.

    1. Mariana Caldas, Ana I. Barbosa, Mrinal Bhattacharya, Rui L. Reis, Vitor M. Correlo. Natural melanin nanoparticles (MNPs) extracted from Sepia officinalis: A cost-effective, chemo-photothermal, synergistic nanoplatform for osteosarcoma treatment. Colloids and Surfaces B: Biointerfaces 2024, 239 , 113937. https://doi.org/10.1016/j.colsurfb.2024.113937
    2. Wei Liu, Yue Sun, Bingshuai Zhou, Yifan Chen, Min Liu, Lin Wang, Manlin Qi, Bailong Liu, Biao Dong. Near-infrared light triggered upconversion nanocomposites with multifunction of enhanced antimicrobial photodynamic therapy and gas therapy for inflammation regulation. Journal of Colloid and Interface Science 2024, 663 , 834-846. https://doi.org/10.1016/j.jcis.2024.02.179

    ACS Sustainable Chemistry & Engineering

    Cite this: ACS Sustainable Chem. Eng. 2024, 12, 6, 2149–2161
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acssuschemeng.3c04911
    Published January 31, 2024
    Copyright © 2024 American Chemical Society

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