A Theragnosis Probe Based on BSA/HSA-Conjugated Biocompatible Fluorescent Silicon Nanomaterials for Simultaneous in Vitro Cholesterol Effluxing and Cellular Imaging of Macrophage CellsClick to copy article linkArticle link copied!
Abstract
Fluorescent silicon NPs (Si-NPs) and 3-mercaptopropionic acid-coated CdS NPs (MPA-NPs) were prepared and conjugated with two different albumin proteins, viz., BSA (B) and HSA (H). The absorption, fluorescence, FTIR, circular dichroism, and gel electrophoresis studies confirmed the conjugation of proteins to NPs. DPPH assay confirmed that the conjugated proteins retained their functional activity even after chemical modifications. The sizes of Si-NPs by TEM were found to be ∼8.7 ± 2 nm, whereas MPA-NPs showed individual particle sizes of ∼4.6 ± 1 nm. The in vitro studies suggested that these NPs were highly biocompatible. The potential of these protein-conjugated NPs in cholesterol effluxing and fluorescence imaging was studied using two different macrophage cell lines, viz., human coronary artery endothelial cells (HCAEC) and human umbilical vein endothelial cells (HUVEC). Results suggested that HSA-conjugated NPs showed better cholesterol effluxing ability and superior penetration toward these treated cells. Intracellular presence of Si-NPs was confirmed by confocal microscopic studies. All these studies unravelled the potential of Si-NPs as a theragnosis probe for future biomedical applications.
Cited By
Smart citations by scite.ai include citation statements extracted from the full text of the citing article. The number of the statements may be higher than the number of citations provided by ACS Publications if one paper cites another multiple times or lower if scite has not yet processed some of the citing articles.
This article is cited by 13 publications.
- Xudong Shi, Kai Gao, Guoxin Zhang, Wenlong Zhang, Xingjiu Yang, Ran Gao. Signal Amplification Pretargeted PET/Fluorescence Imaging Based on Human Serum Albumin-Encapsulated GdF3 Nanoparticles for Diagnosis of Ovarian Cancer. ACS Biomaterials Science & Engineering 2022, 8
(11)
, 4956-4964. https://doi.org/10.1021/acsbiomaterials.2c00374
- Shanka Walia, Ashish K. Shukla, Chandni Sharma, Amitabha Acharya. Engineered Bright Blue- and Red-Emitting Carbon Dots Facilitate Synchronous Imaging and Inhibition of Bacterial and Cancer Cell Progression via 1O2-Mediated DNA Damage under Photoirradiation. ACS Biomaterials Science & Engineering 2019, 5
(4)
, 1987-2000. https://doi.org/10.1021/acsbiomaterials.9b00149
- Rong Yan, Zhangxuan Shou, Jie Chen, Hao Wu, Yuan Zhao, Lin Qiu, Pengju Jiang, Xiao-Zhou Mou, Jianhao Wang, Yong-Qiang Li. On–Off–On Gold Nanocluster-Based Fluorescent Probe for Rapid Escherichia coli Differentiation, Detection and Bactericide Screening. ACS Sustainable Chemistry & Engineering 2018, 6
(4)
, 4504-4509. https://doi.org/10.1021/acssuschemeng.8b00112
- Xuechen Li, Xinyue Li, Suyoung Park, Shining Wu, Yongxian Guo, Ki Taek Nam, Nahyun Kwon, Juyoung Yoon, Qiongzheng Hu. Photodynamic and Photothermal therapy via human serum albumin delivery. Coordination Chemistry Reviews 2024, 520 , 216142. https://doi.org/10.1016/j.ccr.2024.216142
- Shanka Walia, Mohit J. Mehta. Recent progress on nanosystems for nucleic acid delivery. RSC Pharmaceutics 2024, 1
(4)
, 645-674. https://doi.org/10.1039/D4PM00009A
- Bibi Shaguftah Khatoon, Md Abrar Siddiquee, Masood Nadeem, Sana Tanweer, Jahangeer Ahmed, Rajan Patel, M. Moshahid A. Rizvi, Gyandshwar K. Rao, Kamal N. Sharma, Nasimul Hoda, Kamalakanta Behera. Synthesis, characterization, and anticancer activity of 4,6-dichloropyrimidine chalcone hybrids and their mechanistic interaction studies with HSA: In-silico and spectroscopic investigations. Journal of Molecular Structure 2024, 1303 , 137532. https://doi.org/10.1016/j.molstruc.2024.137532
- Xiaokan Yu, Chenming Li, Baozhu Wang, Xinpei Ding, Nan Wang, Bengang Xing, Zhijun Zhang. Protein-mediated fluorescent probes for bioimaging and biosensing: From fundamentals to applications. TrAC Trends in Analytical Chemistry 2024, 170 , 117462. https://doi.org/10.1016/j.trac.2023.117462
- Binbin Chu, Houyu Wang, Yao He. Fluorescent Silicon-based Nanomaterials Imaging Technology in Diseases. Chemical Research in Chinese Universities 2021, 37
(4)
, 880-888. https://doi.org/10.1007/s40242-021-1180-9
- Chandni Sharma, Ashish K. Shukla, Amitabha Acharya. Rational design of a FRET-based nanoprobe of gold-conjugated carbon dots for simultaneous monitoring and disruption of
Pseudomonas aeruginosa
biofilm through selective detection of virulence factor pyocyanin. Environmental Science: Nano 2021, 8
(6)
, 1713-1728. https://doi.org/10.1039/D1EN00187F
- Shanka Walia, Chandni Sharma, Amitabha Acharya. Biocompatible Fluorescent Nanomaterials for Molecular Imaging Applications. 2020, 27-53. https://doi.org/10.1007/978-981-15-4280-0_3
- Chandni Sharma, Shanka Walia, Amitabha Acharya. Nanomaterials for Point of Care Disease Detection. 2020, 55-77. https://doi.org/10.1007/978-981-15-4280-0_4
- Lin Sun, Jie Xie, Zhong Jin. Different Dimensional Nanostructured Silicon Materials: From Synthesis Methodology to Application in High‐Energy Lithium‐Ion Batteries. Energy Technology 2019, 7
(11)
https://doi.org/10.1002/ente.201900962
- Anika Guliani, Amitabha Acharya. Nanomaterials as Protein, Peptide and Gene Delivery Agents. The Open Biotechnology Journal 2018, 12
(1)
, 154-165. https://doi.org/10.2174/1874070701812010154
Article Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.
Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.
The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated.