An Engineered Device for Indoleacetic Acid Production under Quorum Sensing Signals Enables Cupriavidus pinatubonensis JMP134 To Stimulate Plant GrowthClick to copy article linkArticle link copied!
- Ana Zúñiga*Ana Zúñiga*E-mail: [email protected]Facultad de Ingeniería y Ciencias, Universidad Adolfo Ibáñez—Center of Applied Ecology and Sustainability, Santiago de Chile, 2640, ChileCentre de Biochimie Structurale, INSERM U1054, CNRS UMR5048, University of Montpellier, Montpellier, FranceMore by Ana Zúñiga
- Francisco de la FuenteFrancisco de la FuenteFacultad de Ingeniería y Ciencias, Universidad Adolfo Ibáñez—Center of Applied Ecology and Sustainability, Santiago de Chile, 2640, ChileR2B Catalyst, Research Center, Andrés Bello 2299, Santiago, ChileMore by Francisco de la Fuente
- Fernán FedericiFernán FedericiDepartamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Fondo de Desarrollo de Áreas Prioritarias, Center for Genome Regulation, Millennium Institute for Integrative Systems and Synthetic Biology, Pontificia Universidad Católica de Chile, Santiago, ChileMore by Fernán Federici
- Corinne LionneCorinne LionneCentre de Biochimie Structurale, INSERM U1054, CNRS UMR5048, University of Montpellier, Montpellier, FranceMore by Corinne Lionne
- Jérome BônnetJérome BônnetCentre de Biochimie Structurale, INSERM U1054, CNRS UMR5048, University of Montpellier, Montpellier, FranceMore by Jérome Bônnet
- Victor de Lorenzo
- Bernardo GonzálezBernardo GonzálezFacultad de Ingeniería y Ciencias, Universidad Adolfo Ibáñez—Center of Applied Ecology and Sustainability, Santiago de Chile, 2640, ChileMore by Bernardo González
Abstract
The environmental effects of chemical fertilizers and pesticides have encouraged the quest for new strategies to increase crop productivity with minimal impacts on the natural medium. Plant growth promoting rhizobacteria (PGPR) can contribute to this endeavor by improving fitness through better nutrition acquisition and stress tolerance. Using the neutral (non PGPR) rhizobacterium Cupriavidus pinatubonensis JMP134 as the host, we engineered a regulatory forward loop that triggered the synthesis of the phytohormone indole-3-acetic acid (IAA) in a manner dependent on quorum sensing (QS) signals. Implementation of the device in JMP134 yielded synthesis of IAA in an autoregulated manner, improving the growth of the roots of inoculated Arabidopsis thaliana. These results not only demonstrated the value of the designed genetic module, but also validated C. pinatubonensis JMP134 as a suitable vehicle for agricultural applications, as it is amenable to genetic manipulations.
Cited By
Smart citations by scite.ai include citation statements extracted from the full text of the citing article. The number of the statements may be higher than the number of citations provided by ACS Publications if one paper cites another multiple times or lower if scite has not yet processed some of the citing articles.
This article is cited by 22 publications.
- Adhi Singh, Kailash Chand Kumawat. Unraveling the potential of microbial diversity in pesticide remediation: An eco-friendly approach for environmental sustainability. Journal of Agriculture and Food Research 2025, 21 , 101832. https://doi.org/10.1016/j.jafr.2025.101832
- Jyotsana Tilgam, Ritu Vishwakrma, Shaloo Verma, Khushboo Upadhayay, Alok Ranjan. The Biochemistry of Plant-Microbe Interactions in the Rhizosphere. 2025, 149-161. https://doi.org/10.1007/978-981-96-3534-4_7
- Sougata Ghosh, Bishwarup Sarkar, Ajay Kumar. Microbiome nanoengineering to increase crop productivity. 2025, 313-335. https://doi.org/10.1016/B978-0-443-21692-3.00006-9
- Carin J. Ragland, Kevin Y. Shih, José R. Dinneny. Choreographing root architecture and rhizosphere interactions through synthetic biology. Nature Communications 2024, 15
(1)
https://doi.org/10.1038/s41467-024-45272-5
- Tamanna Bhardwaj, Kanika Khanna, Pooja Sharma, Shalini Dhiman, Mohd Ibrahim, Upma Arora, Priyanka Sharma, Indu Sharma, Priya Arora, Ashutosh Sharma, Rupinder Kaur, Bilal Ahmad Mir, Puja Ohri, Renu Bhardwaj. Plant Growth-Promoting Rhizobacteria (PGPR): A Credible Tool for Sustainable Agriculture. 2024, 211-250. https://doi.org/10.2174/9789815179699124010011
- Shweta Sharma, Amit Kumar Kesharwani, Aditya Kulshreshtha. Bioengineered Microbes for Restoration of Soil Health. 2024, 33-47. https://doi.org/10.1007/978-981-99-9482-3_3
- Sekar Hamsa, Ruby Tiwari, Chanderkant Chaudhary. Utility of Biofertilizers for Soil Sustainability. 2023, 293-330. https://doi.org/10.2174/9789815124033123010019
- Smaranika Panda, Kang Zhou. Engineering microbes to overproduce natural products as agrochemicals. Synthetic and Systems Biotechnology 2023, 8
(1)
, 79-85. https://doi.org/10.1016/j.synbio.2022.11.005
- E. Sebastian Gomez-Hinostroza, Nicolás Gurdo, María Victoria Gracia Alvan Vargas, Pablo I. Nikel, María-Eugenia Guazzaroni, Linda P. Guaman, David J. Castillo Cornejo, Raúl Platero, Carlos Barba-Ostria. Current landscape and future directions of synthetic biology in South America. Frontiers in Bioengineering and Biotechnology 2023, 11 https://doi.org/10.3389/fbioe.2023.1069628
- Timothy L. Haskett, Barney A. Geddes, Ponraj Paramasivan, Patrick Green, Samir Chitnavis, Marta D. Mendes, Beatriz Jorrín, Hayley E. Knights, Tahlia R. Bastholm, Joshua P. Ramsay, Giles E. D. Oldroyd, Philip S. Poole. Rhizopine biosensors for plant‐dependent control of bacterial gene expression. Environmental Microbiology 2023, 25
(2)
, 383-396. https://doi.org/10.1111/1462-2920.16288
- Sang-Woo Han, Yasuo Yoshikuni. Microbiome engineering for sustainable agriculture: using synthetic biology to enhance nitrogen metabolism in plant-associated microbes. Current Opinion in Microbiology 2022, 68 , 102172. https://doi.org/10.1016/j.mib.2022.102172
- Christopher M. Dundas, José R. Dinneny. Genetic Circuit Design in Rhizobacteria. BioDesign Research 2022, 2022 , 9858049. https://doi.org/10.34133/2022/9858049
- Rajinder Kaur, Sukhminderjit Kaur, Gurleen Kaur. Molecular and physiological manipulations in rhizospheric bacteria. Acta Physiologiae Plantarum 2021, 43
(5)
https://doi.org/10.1007/s11738-021-03251-z
- Timothy L Haskett, Andrzej Tkacz, Philip S Poole. Engineering rhizobacteria for sustainable agriculture. The ISME Journal 2021, 15
(4)
, 949-964. https://doi.org/10.1038/s41396-020-00835-4
- Vipin Chandra Kalia, Chunjie Gong, Sanjay K. S. Patel, Jung-Kul Lee. Regulation of Plant Mineral Nutrition by Signal Molecules. Microorganisms 2021, 9
(4)
, 774. https://doi.org/10.3390/microorganisms9040774
- Jing Ke, Bing Wang, Yasuo Yoshikuni. Microbiome Engineering: Synthetic Biology of Plant-Associated Microbiomes in Sustainable Agriculture. Trends in Biotechnology 2021, 39
(3)
, 244-261. https://doi.org/10.1016/j.tibtech.2020.07.008
- Ilenne Del Valle, Emily M. Fulk, Prashant Kalvapalle, Jonathan J. Silberg, Caroline A. Masiello, Lauren B. Stadler. Translating New Synthetic Biology Advances for Biosensing Into the Earth and Environmental Sciences. Frontiers in Microbiology 2021, 11 https://doi.org/10.3389/fmicb.2020.618373
- Debanjan Sanyal, G. Venkata Subhash, Vinay Dwivedi, Santanu Dasgupta. Impact of Climate Change on Localized Plant–Microbe Signalling and Technology Advancement in Microbial Quorum Sensing. 2021, 695-715. https://doi.org/10.1007/978-3-030-76863-8_35
- Wen-Dong Xian, Nimaichand Salam, Meng-Meng Li, En-Min Zhou, Yi-Rui Yin, Ze-Tao Liu, Yu-Zhen Ming, Xiao-Tong Zhang, Geng Wu, Lan Liu, Min Xiao, Hong-Chen Jiang, Wen-Jun Li. Network-directed efficient isolation of previously uncultivated Chloroflexi and related bacteria in hot spring microbial mats. npj Biofilms and Microbiomes 2020, 6
(1)
https://doi.org/10.1038/s41522-020-0131-4
- Kristina Stephens, William E. Bentley. Synthetic Biology for Manipulating Quorum Sensing in Microbial Consortia. Trends in Microbiology 2020, 28
(8)
, 633-643. https://doi.org/10.1016/j.tim.2020.03.009
- Agnieszka Kuźniar, Kinga Włodarczyk, Agnieszka Wolińska. Agricultural and Other Biotechnological Applications Resulting from Trophic Plant-Endophyte Interactions. Agronomy 2019, 9
(12)
, 779. https://doi.org/10.3390/agronomy9120779
- Dattatray K. Bedade, Manoj J. Dev, Rekha S. Singhal. Bioreactor studies on acrylamidase produced from Cupriavidus oxalaticus ICTDB921: Production, kinetic modeling, and purification. Biochemical Engineering Journal 2019, 149 , 107245. https://doi.org/10.1016/j.bej.2019.107245
Article Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.
Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.
The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated.