Heterochiral DNA Strand-Displacement Based on Chimeric d/l-Oligonucleotides
- Brian E. YoungBrian E. YoungDepartment of Chemistry, Texas A&M University, College Station, Texas 77843, United StatesMore by Brian E. Young
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- Jonathan T. Sczepanski*Jonathan T. Sczepanski*E-mail: [email protected]Department of Chemistry, Texas A&M University, College Station, Texas 77843, United StatesMore by Jonathan T. Sczepanski
Abstract
Heterochiral DNA strand-displacement reactions enable sequence-specific interfacing of oligonucleotide enantiomers, making it possible to interface native d-nucleic acids with molecular circuits built using nuclease-resistant l-DNA. To date, all heterochiral reactions have relied on peptide nucleic acid (PNA), which places potential limits on the scope and utility of this approach. Herein, we now report heterochiral strand-displacement in the absence of PNA, instead utilizing chimeric d/l-DNA complexes to interface oligonucleotides of the opposite chirality. We show that these strand-displacement reactions can be easily integrated into multicomponent heterochiral circuits, are compatible with both DNA and RNA inputs, and can be engineered to function in serum-supplemented medium. We anticipate that these new reactions will lead to a wider application of heterochiral strand-displacement, especially in the design of biocompatible nucleic acid circuits that can reliably operate within living systems.
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This article is cited by 7 publications.
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- Adam M. Kabza, Jonathan T. Sczepanski. l-DNA-Based Catalytic Hairpin Assembly Circuit. Molecules 2020, 25 (4) , 947. https://doi.org/10.3390/molecules25040947