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Adenosine Triphosphate and Carbon Efficient Route to Second Generation Biofuel Isopentanol

  • Christopher B. Eiben
    Christopher B. Eiben
    Department of Bioengineering, University of California, Berkeley, Berkeley, California 94270, United States
    Department of Bioengineering, University of California, San Francisco, California 94143, United States
  • Tian Tian
    Tian Tian
    Joint BioEnergy Institute, Emeryville, California 94608, United States
    Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States
    More by Tian Tian
  • Mitchell G. Thompson
    Mitchell G. Thompson
    Joint BioEnergy Institute, Emeryville, California 94608, United States
    Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States
    Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, California 94270, United States
  • Daniel Mendez-Perez
    Daniel Mendez-Perez
    Joint BioEnergy Institute, Emeryville, California 94608, United States
    Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States
  • Nurgul Kaplan
    Nurgul Kaplan
    Joint BioEnergy Institute, Emeryville, California 94608, United States
    Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States
    Agile BioFoundry, Emeryville, California 94608, United States
  • Garima Goyal
    Garima Goyal
    Joint BioEnergy Institute, Emeryville, California 94608, United States
    Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States
    Agile BioFoundry, Emeryville, California 94608, United States
    More by Garima Goyal
  • Jennifer Chiniquy
    Jennifer Chiniquy
    Joint BioEnergy Institute, Emeryville, California 94608, United States
    Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States
    Agile BioFoundry, Emeryville, California 94608, United States
  • Nathan J. Hillson
    Nathan J. Hillson
    Joint BioEnergy Institute, Emeryville, California 94608, United States
    Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States
    Agile BioFoundry, Emeryville, California 94608, United States
  • Taek Soon Lee
    Taek Soon Lee
    Joint BioEnergy Institute, Emeryville, California 94608, United States
    Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States
  • , and 
  • Jay D. Keasling*
    Jay D. Keasling
    Department of Bioengineering, University of California, Berkeley, Berkeley, California 94270, United States
    Joint BioEnergy Institute, Emeryville, California 94608, United States
    Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States
    Department of Chemical & Biomolecular Engineering, University of California, Berkeley, Berkeley, California 94270, United States
    Institute for Quantitative Biosciences, University of California, Berkeley, Berkeley, California 94270, United States
    Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kogle Alle, Hørsholm DK2970, Denmark
    Center for Synthetic Biochemistry, Institute for Synthetic Biology, Shenzhen Institutes for Advanced Technologies, Shenzhen 518055, China
Cite this: ACS Synth. Biol. 2020, 9, 3, 468–474
Publication Date (Web):March 9, 2020
https://doi.org/10.1021/acssynbio.9b00402
Copyright © 2020 American Chemical Society

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    Abstract

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    Climate change necessitates the development of CO2 neutral or negative routes to chemicals currently produced from fossil carbon. In this paper we demonstrate a pathway from the renewable resource glucose to next generation biofuel isopentanol by pairing the isovaleryl-CoA biosynthesis pathway from Myxococcus xanthus and a butyryl-CoA reductase from Clostridium acetobutylicum. The best plasmid and Escherichia coli strain combination makes 80.50 ± 8.08 (SD) mg/L of isopentanol after 36 h under microaerobic conditions with an oleyl alcohol overlay. In addition, the system also shows a strong preference for isopentanol production over prenol in microaerobic conditions. Finally, the pathway requires zero adenosine triphosphate and can be paired theoretically with nonoxidative glycolysis, the combination being redox balanced from glucose thus avoiding unnecessary carbon loss as CO2. These pathway properties make the isovaleryl-CoA pathway an attractive isopentanol production route for further optimization.

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    The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acssynbio.9b00402.

    • Sup. Figure 1; Sup. Figure 2 (PDF)

    • Data set_S1 table of contents. Data set_S1: Contains DH1_C5_AlcoholData, eQuilibratorModeling, SupplementalTable1, SupplementalTable2, SupplementalTable3, and SupplementalTable4 (XLSX)

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    Cited By

    This article is cited by 8 publications.

    1. Yilan Liu, Jinjin Chen, David Crisante, Jhoselyn Marisol Jaramillo Lopez, Radhakrishnan Mahadevan. Dynamic Cell Programming with Quorum Sensing-Controlled CRISPRi Circuit. ACS Synthetic Biology 2020, 9 (6) , 1284-1291. https://doi.org/10.1021/acssynbio.0c00148
    2. Mohammad Ali Asadollahi, Sajad Rafatiyan, Meysam Madadi, Fubao Sun. Higher alcohols: metabolic pathways and engineering strategies for enhanced production. 2024, 19-65. https://doi.org/10.1016/B978-0-323-91756-8.00006-2
    3. Samuel Gyebi Arhin, Alessandra Cesaro, Francesco Di Capua, Giovanni Esposito. Recent progress and challenges in biotechnological valorization of lignocellulosic materials: Towards sustainable biofuels and platform chemicals synthesis. Science of The Total Environment 2023, 857 , 159333. https://doi.org/10.1016/j.scitotenv.2022.159333
    4. David N. Carruthers, Taek Soon Lee. Diversifying Isoprenoid Platforms via Atypical Carbon Substrates and Non-model Microorganisms. Frontiers in Microbiology 2021, 12 https://doi.org/10.3389/fmicb.2021.791089
    5. Weerawat Runguphan, Kittapong Sae-Tang, Sutipa Tanapongpipat. Recent advances in the microbial production of isopentanol (3-Methyl-1-butanol). World Journal of Microbiology and Biotechnology 2021, 37 (6) https://doi.org/10.1007/s11274-021-03074-7
    6. Hong Liang, Xiaoqiang Ma, Wenbo Ning, Yurou Liu, Anthony J. Sinskey, Gregory Stephanopoulos, Kang Zhou. Constructing an ethanol utilization pathway in Escherichia coli to produce acetyl-CoA derived compounds. Metabolic Engineering 2021, 65 , 223-231. https://doi.org/10.1016/j.ymben.2020.11.010
    7. Neha Srivastava, Akshay Shrivastav, Rajeev Singh, Mohammed Abohashrh, K. R. Srivastava, Safia Irfan, Manish Srivastava, P. K. Mishra, Vijai Kumar Gupta, Vijay Kumar Thakur. Advances in the Structural Composition of Biomass: Fundamental and Bioenergy Applications. Journal of Renewable Materials 2021, 9 (4) , 615-636. https://doi.org/10.32604/jrm.2021.014374
    8. Liya Liang, Rongming Liu, Emily F. Freed, Carrie A. Eckert. Synthetic Biology and Metabolic Engineering Employing Escherichia coli for C2–C6 Bioalcohol Production. Frontiers in Bioengineering and Biotechnology 2020, 8 https://doi.org/10.3389/fbioe.2020.00710

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