Adenosine Triphosphate and Carbon Efficient Route to Second Generation Biofuel Isopentanol
- Christopher B. EibenChristopher B. EibenDepartment of Bioengineering, University of California, Berkeley, Berkeley, California 94270, United StatesDepartment of Bioengineering, University of California, San Francisco, California 94143, United StatesMore by Christopher B. Eiben
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- Tian TianTian TianJoint BioEnergy Institute, Emeryville, California 94608, United StatesBiological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United StatesMore by Tian Tian
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- Mitchell G. ThompsonMitchell G. ThompsonJoint BioEnergy Institute, Emeryville, California 94608, United StatesBiological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United StatesDepartment of Plant and Microbial Biology, University of California, Berkeley, Berkeley, California 94270, United StatesMore by Mitchell G. Thompson
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- Daniel Mendez-PerezDaniel Mendez-PerezJoint BioEnergy Institute, Emeryville, California 94608, United StatesBiological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United StatesMore by Daniel Mendez-Perez
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- Nurgul KaplanNurgul KaplanJoint BioEnergy Institute, Emeryville, California 94608, United StatesBiological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United StatesAgile BioFoundry, Emeryville, California 94608, United StatesMore by Nurgul Kaplan
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- Garima GoyalGarima GoyalJoint BioEnergy Institute, Emeryville, California 94608, United StatesBiological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United StatesAgile BioFoundry, Emeryville, California 94608, United StatesMore by Garima Goyal
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- Jennifer ChiniquyJennifer ChiniquyJoint BioEnergy Institute, Emeryville, California 94608, United StatesBiological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United StatesAgile BioFoundry, Emeryville, California 94608, United StatesMore by Jennifer Chiniquy
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- Nathan J. HillsonNathan J. HillsonJoint BioEnergy Institute, Emeryville, California 94608, United StatesBiological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United StatesAgile BioFoundry, Emeryville, California 94608, United StatesMore by Nathan J. Hillson
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- Taek Soon LeeTaek Soon LeeJoint BioEnergy Institute, Emeryville, California 94608, United StatesBiological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United StatesMore by Taek Soon Lee
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- Jay D. Keasling*Jay D. KeaslingDepartment of Bioengineering, University of California, Berkeley, Berkeley, California 94270, United StatesJoint BioEnergy Institute, Emeryville, California 94608, United StatesBiological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United StatesDepartment of Chemical & Biomolecular Engineering, University of California, Berkeley, Berkeley, California 94270, United StatesInstitute for Quantitative Biosciences, University of California, Berkeley, Berkeley, California 94270, United StatesNovo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kogle Alle, Hørsholm DK2970, DenmarkCenter for Synthetic Biochemistry, Institute for Synthetic Biology, Shenzhen Institutes for Advanced Technologies, Shenzhen 518055, ChinaMore by Jay D. Keasling
Abstract
Climate change necessitates the development of CO2 neutral or negative routes to chemicals currently produced from fossil carbon. In this paper we demonstrate a pathway from the renewable resource glucose to next generation biofuel isopentanol by pairing the isovaleryl-CoA biosynthesis pathway from Myxococcus xanthus and a butyryl-CoA reductase from Clostridium acetobutylicum. The best plasmid and Escherichia coli strain combination makes 80.50 ± 8.08 (SD) mg/L of isopentanol after 36 h under microaerobic conditions with an oleyl alcohol overlay. In addition, the system also shows a strong preference for isopentanol production over prenol in microaerobic conditions. Finally, the pathway requires zero adenosine triphosphate and can be paired theoretically with nonoxidative glycolysis, the combination being redox balanced from glucose thus avoiding unnecessary carbon loss as CO2. These pathway properties make the isovaleryl-CoA pathway an attractive isopentanol production route for further optimization.
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