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An Escherichia coli Chassis for Production of Electrically Conductive Protein Nanowires

  • Toshiyuki Ueki
    Toshiyuki Ueki
    Department of Microbiology, University of Massachusetts-Amherst, Amherst, Massachusetts 01003, United States
    Institute for Applied Life Sciences, University of Massachusetts-Amherst, Amherst, Massachusetts 01003, United States
    More by Toshiyuki Ueki
  • , 
  • David J. F. Walker
    David J. F. Walker
    Department of Microbiology, University of Massachusetts-Amherst, Amherst, Massachusetts 01003, United States
    Institute for Applied Life Sciences, University of Massachusetts-Amherst, Amherst, Massachusetts 01003, United States
    More by David J. F. Walker
    Orcidhttp://orcid.org/0000-0003-1354-0543
  • , 
  • Trevor L. Woodard
    Trevor L. Woodard
    Department of Microbiology, University of Massachusetts-Amherst, Amherst, Massachusetts 01003, United States
    More by Trevor L. Woodard
  • , 
  • Kelly P. Nevin
    Kelly P. Nevin
    Department of Microbiology, University of Massachusetts-Amherst, Amherst, Massachusetts 01003, United States
    More by Kelly P. Nevin
  • , 
  • Stephen S. Nonnenmann
    Stephen S. Nonnenmann
    Institute for Applied Life Sciences, University of Massachusetts-Amherst, Amherst, Massachusetts 01003, United States
    Department of Mechanical and Industrial Engineering, University of Massachusetts-Amherst, Amherst, Massachusetts 01003, United States
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    Orcidhttp://orcid.org/0000-0002-5369-9279
  • , and 
  • Derek R. Lovley*
    Derek R. Lovley
    Department of Microbiology, University of Massachusetts-Amherst, Amherst, Massachusetts 01003, United States
    Institute for Applied Life Sciences, University of Massachusetts-Amherst, Amherst, Massachusetts 01003, United States
    *Email: [email protected]
    More by Derek R. Lovley
    Orcidhttp://orcid.org/0000-0001-7158-3555
Cite this: ACS Synth. Biol. 2020, 9, 3, 647–654
Publication Date (Web):March 3, 2020
https://doi.org/10.1021/acssynbio.9b00506
Copyright © 2020 American Chemical Society
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    Abstract

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    Geobacter sulfurreducens’ pilin-based electrically conductive protein nanowires (e-PNs) are a revolutionary electronic material. They offer novel options for electronic sensing applications and have the remarkable ability to harvest electrical energy from atmospheric humidity. However, technical constraints limit mass cultivation and genetic manipulation of G. sulfurreducens. Therefore, we designed a strain of Escherichia coli to express e-PNs by introducing a plasmid that contained an inducible operon with E. coli genes for type IV pili biogenesis machinery and a synthetic gene designed to yield a peptide monomer that could be assembled into e-PNs. The e-PNs expressed in E. coli and harvested with a simple filtration method had the same diameter (3 nm) and conductance as e-PNs expressed in G. sulfurreducens. These results, coupled with the robustness of E. coli for mass cultivation and the extensive E. coli toolbox for genetic manipulation, greatly expand the opportunities for large-scale fabrication of novel e-PNs.

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