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Reagents for Astatination of Biomolecules. 4. Comparison of Maleimido-closo-Decaborate(2-) and meta-[211At]Astatobenzoate Conjugates for Labeling anti-CD45 Antibodies with [211At]Astatine

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    Reagents for Astatination of Biomolecules. 4. Comparison of Maleimido-closo-Decaborate(2-) and meta-[211At]Astatobenzoate Conjugates for Labeling anti-CD45 Antibodies with [211At]Astatine
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    Departments of Radiation Oncology, Medicine, Biological Structure, and Pediatrics, University of Washington, Seattle, Washington, and Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, Washington
    * Corresponding author. D. Scott Wilbur, Department of Radiation Oncology, University of Washington, Box 355016, 616 N. E. Northlake Place, Seattle, WA 98105. Phone: 206-616-9246. Fax: 206-616-8798. [email protected]
    †Department of Radiation Oncology, University of Washington.
    ‡Department of Medicine, University of Washington.
    §Department of Biological Structure, University of Washington.
    ∥Department of Pediatrics, University of Washington.
    ⊥Fred Hutchinson Cancer Research Center.
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    Bioconjugate Chemistry

    Cite this: Bioconjugate Chem. 2009, 20, 10, 1983–1991
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    https://doi.org/10.1021/bc9000799
    Published September 4, 2009
    Copyright © 2009 American Chemical Society
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    Abstract

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    An investigation was conducted to compare the in vivo tissue distribution of a rat antimurine CD45 monoclonal antibody (30F11) and an irrelevant mAbs (CA12.10C12) labeled with 211At using two different labeling methods. In the investigation, the mAbs were also labeled with 125I to assess the in vivo stability of the labeling methods toward deastatination. One labeling method employed N-hydroxysuccinimidyl meta-[211At]astatobenzoate, [211At]1c, and N-hydroxysuccinimidyl meta-[125I]iodobenzoate, [125I]1b, in conjugation reactions to obtain the radiolabeled mAbs. The other labeling method involved conjugation of a maleimido-closo-decaborate(2-) derivative, 2, with sulfhydryl groups on the mAbs, followed by labeling of the mAb-2 conjugates using Na[211At]At or Na[125I]I and chloramine-T. Concentrations of the 211At/125I pair of radiolabeled mAbs in selected tissues were examined in BALB/c mice at 1, 4, and 24 h post injection (pi). The co-injected anti-CD45 mAb, 30F11, labeled with [125I]1b and [211At]1c targeted the CD45-bearing cells in the spleen with the percent injected dose (%ID) of 125I in that tissue being 13.31 ± 0.78; 17.43 ± 2.56; 5.23 ± 0.50; and 211At being 6.56 ± 0.40; 10.14 ± 1.49; 7.52 ± 0.79 at 1, 4, and 24 h pi (respectively). However, better targeting (or retention) of the 125I and 211At was obtained for 30F11 conjugated with the closo-decaborate(2-), 2. The %ID in the spleen of 125I (i.e., [125I]30F11-2) being 21.15 ± 1.33; 22.22 ± 1.95; 12.41 ± 0.75; and 211At (i.e., [211At]30F11-2) being 22.78 ± 1.29; 25.05 ± 2.35; 17.30 ± 1.20 at 1, 4, and 24 h pi (respectively). In contrast, the irrelevant mAb, CA12.10C12, labeled with 125I or 211At by either method had less than 0.8% ID in the spleen at any time point, except for [211At]CA12.10C12-1c, which had 1.62 ± 0.14%ID and 1.21 ± 0.08%ID at 1 and 4 h pi. The higher spleen concentrations in that conjugate appear to be due to in vivo deastatination. Differences in 125I and 211At concentrations in lung, neck, and stomach indicate that the meta-[211At]benzoyl conjugates underwent deastatination, whereas the 211At-labeled closo-decaborate(2-) conjugates were very stable to in vivo deastatination. In summary, using the closo-decaborate(2-) 211At labeling approach resulted in higher concentrations of 211At in target tissue (spleen) and higher stability to in vivo deastatination in this model. These findings, along with the simpler and higher-yielding 211At-labeling method, provide the basis for using the closo-decaborate(2-) labeling reagent, 2, in our continued studies of the application of 211At-labeled mAbs for conditioning in hematopoietic cell transplantation.

    Copyright © 2009 American Chemical Society

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    Supporting Information

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    Tables (S1−S5) containing biodistribution data, either %ID/g or %ID, in selected tissues, and copies of UV- and radiochromatograms (Figures S1−S4) for [211At]1c and [125I]1b. This material is available free of charge via the Internet at http://pubs.acs.org.

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    Bioconjugate Chemistry

    Cite this: Bioconjugate Chem. 2009, 20, 10, 1983–1991
    Click to copy citationCitation copied!
    https://doi.org/10.1021/bc9000799
    Published September 4, 2009
    Copyright © 2009 American Chemical Society
    Request reuse permissions

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