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Comparison of the C2A Domain of Synaptotagmin-I and Annexin-V As Probes for Detecting Cell Death

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    Comparison of the C2A Domain of Synaptotagmin-I and Annexin-V As Probes for Detecting Cell Death
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    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, United Kingdom, and Cancer Research UK Cambridge Research Institute, Li-Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, United Kingdom
    * Corresponding author. Kevin M. Brindle, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK., Tel: +44 (0)1223 333674, Fax: +44 (0)1223 766002, Email: [email protected]
    †Equal contributions.
    §Cancer Research UK Cambridge Research Institute.
    ‡University of Cambridge.
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    Bioconjugate Chemistry

    Cite this: Bioconjugate Chem. 2010, 21, 5, 884–891
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    https://doi.org/10.1021/bc9004415
    Published April 19, 2010
    Copyright © 2010 American Chemical Society
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    Abstract

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    The induction of apoptosis is frequently accompanied by the exposure of phosphatidylserine (PS) on the cell surface, which has been detected using radionuclide and fluorescently labeled derivatives of the PS-binding protein, Annexin V. The fluorescently labeled protein has been used extensively in vitro as a diagnostic reagent for detecting cell death, and radionuclide-labeled derivatives have undergone clinical trials for detecting tumor cell death in vivo following treatment. We show here that the C2A domain of Synaptotagmin-I, which had been fluorescently labeled at a single cysteine residue introduced by site-directed mutagenesis, detected the same levels of cell death as a similarly labeled Annexin-V derivative, in drug-treated murine lymphoma and human breast cancer cell lines in vitro. However, the C2A derivative showed significantly less binding to viable cells and, as a consequence, up to 4-fold more specific binding to apoptotic and necrotic cells when compared with Annexin-V. C2A offers a potential route for the development of a new generation of more specific imaging probes for the detection of tumor cell death in the clinic.

    Copyright © 2010 American Chemical Society

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    Supporting Information

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    Expression and purification of C2A(S78C), the protein assay, electrospray ionization (ESI) mass spectrometry, and surface plasmon resonance (SPR) measurements are described in more detail. There is a table showing the dissociation constants derived from affinity and kinetic analysis of the SPR data, a figure showing the ESI−mass spectrum of the C2Am-AlxF647 conjugate, figures showing the SDS-PAGE gels of the C2Am-AlxF647 and AnxV-AlxF647 conjugates and a figure demonstrating the effect of probe concentration on the specificity of labeling with C2Am and AnxV. This material is available free of charge via the Internet at http://pubs.acs.org.

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    Bioconjugate Chemistry

    Cite this: Bioconjugate Chem. 2010, 21, 5, 884–891
    Click to copy citationCitation copied!
    https://doi.org/10.1021/bc9004415
    Published April 19, 2010
    Copyright © 2010 American Chemical Society
    Request reuse permissions

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