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Novel Functional Interactions between Nucleotide Excision DNA Repair Proteins Influencing the Enzymatic Activities of TFIIH, XPG, and ERCC1-XPF

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    Novel Functional Interactions between Nucleotide Excision DNA Repair Proteins Influencing the Enzymatic Activities of TFIIH, XPG, and ERCC1-XPF†
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    Department of Cell Biology and Genetics, Medical Genetics Center, Center of Biomedical Genetics, Erasmus University, P.O. Box 1738, 3000 DR Rotterdam, The Netherlands
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    Biochemistry

    Cite this: Biochemistry 2001, 40, 1, 160–165
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    https://doi.org/10.1021/bi002021b
    Published December 5, 2000
    Copyright © 2001 American Chemical Society
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    Abstract

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    The multisubunit basal transcription factor IIH (TFIIH) has a dual involvement in nucleotide excision repair (NER) of a variety of DNA lesions, including UV-induced photoproducts, and RNA polymerase II transcription. In both processes, TFIIH is implicated with local DNA unwinding, which is attributed to its helicase subunits XPB and XPD. To further define the role of TFIIH in NER, functional interactions between TFIIH and other DNA repair proteins were analyzed. We show that the TFIIH-associated ATPase activity is stimulated by both XPA and the XPC-HR23B complex. However, while XPA promotes the ATPase activity specifically in the presence of damaged DNA, stimulation by XPC-HR23B is lesion independent. Furthermore, we reveal that TFIIH inhibits the structure-specific endonuclease activities of both XPG and ERCC1-XPF, responsible for the 3‘ and 5‘ incision in NER, respectively. The inhibition occurs in the absence of ATP and is reversed upon addition of ATP. These results point toward additional roles for TFIIH and ATP during NER distinct from a requirement for DNA unwinding in the regulation of the endonuclease activities of XPG and ERCC1-XPF.

    Copyright © 2001 American Chemical Society

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    †

    This work was funded by The Netherlands Organization for Scientific Research Section Medical Sciences, Grant 901-151-01. K.S. was supported by the Biodesign Research Program Grant from the Institute of Physical and Chemical Research (RIKEN).

    ‡

     Present address:  Imperial Cancer Research Fund, Clare Hall Laboratories, Blanche Lane, South Mimms, EN6 3LD, U.K.

    §

     Present address:  Cellular Physiology Laboratory, The Institute of Physical and Chemical Research (RIKEN), 2-1 Hirosawa, Wako, Saitama 351-01, Japan.

    *

     Corresponding author. Telephone:  +31 10 408 7199. Fax:  +31 10 408 9468. E-mail:  [email protected].

    Cited By

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    This article is cited by 17 publications.

    1. Ludovic C. J. Gillet, Orlando D. Schärer. Molecular Mechanisms of Mammalian Global Genome Nucleotide Excision Repair. Chemical Reviews 2006, 106 (2) , 253-276. https://doi.org/10.1021/cr040483f
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    7. Devashish Das, Gert E. Folkers, Marc van Dijk, Nicolaas G.J. Jaspers, Jan H.J. Hoeijmakers, Robert Kaptein, Rolf Boelens. The Structure of the XPF-ssDNA Complex Underscores the Distinct Roles of the XPF and ERCC1 Helix- Hairpin-Helix Domains in ss/ds DNA Recognition. Structure 2012, 20 (4) , 667-675. https://doi.org/10.1016/j.str.2012.02.009
    8. Christophe Rouillon, Malcolm F. White. The XBP-Bax1 Helicase-Nuclease Complex Unwinds and Cleaves DNA. Journal of Biological Chemistry 2010, 285 (14) , 11013-11022. https://doi.org/10.1074/jbc.M109.094763
    9. María Moriel-Carretero, Andrés Aguilera. A Postincision-Deficient TFIIH Causes Replication Fork Breakage and Uncovers Alternative Rad51- or Pol32-Mediated Restart Mechanisms. Molecular Cell 2010, 37 (5) , 690-701. https://doi.org/10.1016/j.molcel.2010.02.008
    10. Frédéric Coin, Valentyn Oksenych, Vincent Mocquet, Stefanie Groh, Christine Blattner, Jean Marc Egly. Nucleotide Excision Repair Driven by the Dissociation of CAK from TFIIH. Molecular Cell 2008, 31 (1) , 9-20. https://doi.org/10.1016/j.molcel.2008.04.024
    11. Vincent Mocquet, Jean Philippe Lainé, Thilo Riedl, Zhou Yajin, Marietta Y Lee, Jean Marc Egly. Sequential recruitment of the repair factors during NER: the role of XPG in initiating the resynthesis step. The EMBO Journal 2008, 27 (1) , 155-167. https://doi.org/10.1038/sj.emboj.7601948
    12. Travis J. O'Brien, Bradford R. Brooks, Steven R. Patierno. Nucleotide excision repair functions in the removal of chromium-induced DNA damage in mammalian cells. Molecular and Cellular Biochemistry 2005, 279 (1-2) , 85-95. https://doi.org/10.1007/s11010-005-8225-0
    13. Virginie Gervais, Valérie Lamour, Anass Jawhari, Florent Frindel, Emeric Wasielewski, Sandy Dubaele, Jean-Marc Egly, Jean-Claude Thierry, Bruno Kieffer, Arnaud Poterszman. TFIIH contains a PH domain involved in DNA nucleotide excision repair. Nature Structural & Molecular Biology 2004, 11 (7) , 616-622. https://doi.org/10.1038/nsmb782
    14. Brian S. Thoma, Karen M. Vasquez. Critical DNA damage recognition functions of XPC‐hHR23B and XPA‐RPA in nucleotide excision repair. Molecular Carcinogenesis 2003, 38 (1) , 1-13. https://doi.org/10.1002/mc.10143
    15. Alexander D Guminski, Paul R Harnett, Anna deFazio. Scientists and clinicians test their metal–back to the future with platinum compounds. The Lancet Oncology 2002, 3 (5) , 312-318. https://doi.org/10.1016/S1470-2045(02)00733-7
    16. Anne Stary, Alain Sarasin. The genetics of the hereditary xeroderma pigmentosum syndrome. Biochimie 2002, 84 (1) , 49-60. https://doi.org/10.1016/S0300-9084(01)01358-X
    17. Jaan-Olle Andressoo, Jan H.J. Hoeijmakers, Harm Waard. Nucleotide Excision Repair and its Connection with Cancer and Ageing. , 45-83. https://doi.org/10.1007/1-4020-3764-3_3
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    Biochemistry

    Cite this: Biochemistry 2001, 40, 1, 160–165
    Click to copy citationCitation copied!
    https://doi.org/10.1021/bi002021b
    Published December 5, 2000
    Copyright © 2001 American Chemical Society
    Request reuse permissions

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