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Expression and Characterization of the Isolated Glycosyltransferase Module of Escherichia coli PBP1b

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    Expression and Characterization of the Isolated Glycosyltransferase Module of Escherichia coli PBP1b†
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    Department of Chemistry, Princeton University, Princeton, New Jersey 08544
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    Biochemistry

    Cite this: Biochemistry 2004, 43, 38, 12375–12381
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    https://doi.org/10.1021/bi049142m
    Published September 3, 2004
    Copyright © 2004 American Chemical Society
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    Abstract

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    The enzymes involved in the biosynthesis of peptidoglycan are targets for the development of new antibiotics. The bifunctional high molecular weight (HMW) penicillin-binding proteins (PBPs), which contain both glycosyltransferase (GTase) and transpeptidase (TPase) activities, are particularly attractive targets because of their extracellular location. However, there is limited mechanistic or structural information about the GTase modules of these enzymes. In this paper, we describe the overexpression and characterization of the GTase module of Escherichia coli PBP1b, a paradigm of the HMW PBPs. We define the C-terminal boundary of the GTase module and show that the isolated module can be overexpressed at significantly higher levels than the full-length protein. The catalytic efficiency and other characteristics of the isolated module are comparable in most respects to the full-length enzyme. This work lays the groundwork for mechanistic and structural analysis of GTase modules.

    Copyright © 2004 American Chemical Society

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    †

     Financial support was provided by the National Institute of Health (NIH Grant R01AI50855).

    *

     To whom correspondence should be addressed. Phone:  (617) 432-5488. Fax:  (617) 496-0215. E-mail:  [email protected].

    Cited By

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    This article is cited by 31 publications.

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    22. Andrew L. Lovering, Liza H. de Castro, Daniel Lim, Natalie C. J. Strynadka. Structural Insight into the Transglycosylation Step of Bacterial Cell-Wall Biosynthesis. Science 2007, 315 (5817) , 1402-1405. https://doi.org/10.1126/science.1136611
    23. Haitian Liu, Chi-Huey Wong. Characterization of a transglycosylase domain of Streptococcus pneumoniae PBP1b. Bioorganic & Medicinal Chemistry 2006, 14 (21) , 7187-7195. https://doi.org/10.1016/j.bmc.2006.06.058
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    25. Pauline Macheboeuf, Carlos Contreras-Martel, Viviana Job, Otto Dideberg, Andréa Dessen. Penicillin Binding Proteins: key players in bacterial cell cycle and drug resistance processes. FEMS Microbiology Reviews 2006, 30 (5) , 673-691. https://doi.org/10.1111/j.1574-6976.2006.00024.x
    26. Julien Offant, François Michoux, Annabelle Dermiaux, Jacques Biton, Yves Bourne. Functional characterization of the glycosyltransferase domain of penicillin-binding protein 1a from Thermotoga maritima. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 2006, 1764 (6) , 1036-1042. https://doi.org/10.1016/j.bbapap.2006.03.012
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    Biochemistry

    Cite this: Biochemistry 2004, 43, 38, 12375–12381
    Click to copy citationCitation copied!
    https://doi.org/10.1021/bi049142m
    Published September 3, 2004
    Copyright © 2004 American Chemical Society
    Request reuse permissions

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