ACS Publications. Most Trusted. Most Cited. Most Read
My Activity

Figure 1Loading Img

Staphylococcus aureus Sortase Transpeptidase SrtA:  Insight into the Kinetic Mechanism and Evidence for a Reverse Protonation Catalytic Mechanism

View Author Information
Department of Biochemistry and Biophysics and Johnson Research Foundation, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104-6059, and Department of Chemistry, University of Illinois, Urbana, Illinois 61801
Cite this: Biochemistry 2005, 44, 33, 11188–11200
Publication Date (Web):July 29, 2005
Copyright © 2005 American Chemical Society

    Article Views





    Read OnlinePDF (313 KB)


    Abstract Image

    The Staphylococcus aureus transpeptidase SrtA catalyzes the covalent attachment of LPXTG-containing virulence and colonization-associated proteins to cell-wall peptidoglycan in Gram-positive bacteria. Recent structural characterizations of staphylococcal SrtA, and related transpeptidases SrtB from S. aureus and Bacillus anthracis, provide many details regarding the active site environment, yet raise questions with regard to the nature of catalysis and active site cysteine thiol activation. Here we re-evaluate the kinetic mechanism of SrtA and shed light on aspects of its catalytic mechanism. Using steady-state, pre-steady-state, bisubstrate kinetic studies, and high-resolution electrospray mass spectrometry, revised steady-state kinetic parameters and a ping-pong hydrolytic shunt kinetic mechanism were determined for recombinant SrtA. The pH dependencies of kinetic parameters kcat/Km and kcat for the substrate Abz-LPETG-Dap(Dnp)-NH2 were bell-shaped with pKa values of 6.3 ± 0.2 and 9.4 ± 0.2 for kcat and 6.2 ± 0.2 and 9.4 ± 0.2 for kcat/Km. Solvent isotope effect (SIE) measurements revealed inverse behavior, with a D2Okcat of 0.89 ± 0.01 and a D2O(kcat/Km) of 0.57 ± 0.03 reflecting an equilibrium SIE. In addition, SIE measurements strongly implicated Cys184 participation in the isotope-sensitive rate-determining chemical step when considered in conjunction with an inverse linear proton inventory for kcat. Last, the pH dependence of SrtA inactivation by iodoacetamide revealed a single ionization for inactivation. These studies collectively provide compelling evidence for a reverse protonation mechanism where a small fraction (ca. 0.06%) of SrtA is competent for catalysis at physiological pH, yet is highly active with an estimated kcat/Km of >105 M-1 s-1.

     This work was supported by NIH Grants AI46611 (to D.G.M.) and GM067725 (to N.L.K.) and an NSF Predoctoral Fellowship to B.A.F.

     University of Pennsylvania School of Medicine.


     University of Illinois.


     To whom correspondence should be addressed:  Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, 905A Stellar-Chance Building, 422 Curie Blvd., Philadelphia, PA 19104-6059. Phone:  (215) 898-7619. Fax:  (215) 573-8052. E-mail:  [email protected].

    Cited By

    This article is cited by 110 publications.

    1. Eugene M. Obeng, David L. Steer, Alex Fulcher, Kylie M. Wagstaff. Steric-Deficient Oligoglycine Surrogates Facilitate Multivalent and Bifunctional Nanobody Synthesis via Combined Sortase A Transpeptidation and Click Chemistry. Bioconjugate Chemistry 2023, 34 (9) , 1667-1678.
    2. Jia-Liang Chen, Xiao Wang, Feng Yang, Bin Li, Gottfried Otting, Xun-Cheng Su. 3D Structure of the Transient Intermediate of the Enzyme–Substrate Complex of Sortase A Reveals How Calcium Binding and Substrate Recognition Cooperate in Substrate Activation. ACS Catalysis 2023, 13 (17) , 11610-11624.
    3. Linghui Qian, Xuefen Lin, Xue Gao, Rizwan Ullah Khan, Jia-Yu Liao, Shubo Du, Jingyan Ge, Su Zeng, Shao Q. Yao. The Dawn of a New Era: Targeting the “Undruggables” with Antibody-Based Therapeutics. Chemical Reviews 2023, 123 (12) , 7782-7853.
    4. Jiahua Deng, Qiang Cui. Reverse Protonation of Buried Ion-Pairs in Staphylococcal Nuclease Mutants. Journal of Chemical Theory and Computation 2021, 17 (7) , 4550-4563.
    5. Zhi Zou, Maximilian Nöth, Felix Jakob, Ulrich Schwaneberg. Designed Streptococcus pyogenes Sortase A Accepts Branched Amines as Nucleophiles in Sortagging. Bioconjugate Chemistry 2020, 31 (11) , 2476-2481.
    6. Christopher K. Sue, Scott A. McConnell, Ken Ellis-Guardiola, John M. Muroski, Rachel A. McAllister, Justin Yu, Ana I. Alvarez, Chungyu Chang, Rachel R. Ogorzalek Loo, Joseph A. Loo, Hung Ton-That, Robert T. Clubb. Kinetics and Optimization of the Lysine–Isopeptide Bond Forming Sortase Enzyme from Corynebacterium diphtheriae. Bioconjugate Chemistry 2020, 31 (6) , 1624-1634.
    7. Amy M. Weeks, James A. Wells. Subtiligase-Catalyzed Peptide Ligation. Chemical Reviews 2020, 120 (6) , 3127-3160.
    8. Han Gu, Saptarshi Ghosh, Richard J. Staples, Susan L. Bane. β-Hydroxy-Stabilized Boron–Nitrogen Heterocycles Enable Rapid and Efficient C-Terminal Protein Modification. Bioconjugate Chemistry 2019, 30 (10) , 2604-2613.
    9. Juan Zhang, Menglin Wang, Rui Tang, Yanan Liu, Chunyang Lei, Yan Huang, Zhou Nie, Shouzhuo Yao. Transpeptidation-Mediated Assembly of Tripartite Split Green Fluorescent Protein for Label-Free Assay of Sortase Activity. Analytical Chemistry 2018, 90 (5) , 3245-3252.
    10. Chasper Puorger, Salvatore Di Girolamo, and Georg Lipps . Elucidation of the Recognition Sequence of Sortase B from Bacillus anthracis by Using a Newly Developed Liquid Chromatography–Mass Spectrometry-Based Method. Biochemistry 2017, 56 (21) , 2641-2650.
    11. Tora Biswas, Vijaykumar S. Pawale, Devapriya Choudhury, and Rajendra P. Roy . Sorting of LPXTG Peptides by Archetypal Sortase A: Role of Invariant Substrate Residues in Modulating the Enzyme Dynamics and Conformational Signature of a Productive Substrate. Biochemistry 2014, 53 (15) , 2515-2524.
    12. John M. Robbins and Holly R. Ellis . Steady-State Kinetic Isotope Effects Support a Complex Role of Arg226 in the Proposed Desulfonation Mechanism of Alkanesulfonate Monooxygenase. Biochemistry 2014, 53 (1) , 161-168.
    13. Scott A. Robson, Alex W. Jacobitz, Martin L. Phillips, and Robert T. Clubb . Solution Structure of the Sortase Required for Efficient Production of Infectious Bacillus anthracis Spores. Biochemistry 2012, 51 (40) , 7953-7963.
    14. Bo-Xue Tian and Leif A. Eriksson . Catalytic Mechanism and Roles of Arg197 and Thr183 in the Staphylococcus aureus Sortase A Enzyme. The Journal of Physical Chemistry B 2011, 115 (44) , 13003-13011.
    15. Jeffrey A. Melvin, Christine F. Murphy, Laura G. Dubois, J. Will Thompson, M. Arthur Moseley, and Dewey G. McCafferty . Staphylococcus aureus Sortase A Contributes to the Trojan Horse Mechanism of Immune Defense Evasion with Its Intrinsic Resistance to Cys184 Oxidation. Biochemistry 2011, 50 (35) , 7591-7599.
    16. Justin E. Jones, Christina J. Dreyton, Heather Flick, Corey P. Causey, and Paul R. Thompson . Mechanistic Studies of Agmatine Deiminase from Multiple Bacterial Species. Biochemistry 2010, 49 (43) , 9413-9423.
    17. Bryan Knuckley, Corey P. Causey, Justin E. Jones, Monica Bhatia, Christina J. Dreyton, Tanesha C. Osborne, Hidenari Takahara and Paul R. Thompson . Substrate Specificity and Kinetic Studies of PADs 1, 3, and 4 Identify Potent and Selective Inhibitors of Protein Arginine Deiminase 3. Biochemistry 2010, 49 (23) , 4852-4863.
    18. Clothilde Manzano, Thierry Izoré, Viviana Job, Anne Marie Di Guilmi and Andréa Dessen. Sortase Activity Is Controlled by a Flexible Lid in the Pilus Biogenesis Mechanism of Gram-Positive Pathogens,. Biochemistry 2009, 48 (44) , 10549-10557.
    19. Gang Liu, Fengli Gao, Xiupei Yang, Jingyi Zhang, Suling Yang, Yuanyuan Li, Lin Liu. Aggregation-induced emission for the detection of peptide ligases with improving ligation efficiency. Analytica Chimica Acta 2023, 1284 , 341994.
    20. Yanqu Cai, Die Chen, Yongqi Chen, Ting Li, Lu Wang, Jinzhu Jiang, Zhenzhong Guo, Nicole Jaffrezic-Renault, Zhipeng Zhang, Siyu Huang. An electrochemical biosensor based on graphene intercalated functionalized black phosphorus/gold nanoparticles nanocomposites for the detection of bacterial enzyme. Microchemical Journal 2023, 193 , 109255.
    21. Lili Tian, Li Wang, Fengying Yang, Tiezhong Zhou, Hong Jiang. Exploring the Modulatory Impact of Isosakuranetin on Staphylococcus aureus : Inhibition of Sortase A Activity and α-hemolysin Expression. Virulence 2023, 6
    22. Feng Jiang, Chengteng Cai, Xiumin Wang, Shoufa Han. A dual biomarker-targeting probe enables signal-on surface labeling of Staphylococcus aureus. Bioorganic & Medicinal Chemistry Letters 2023, 93 , 129428.
    23. Eugene M. Obeng, Alex J. Fulcher, Kylie M. Wagstaff. Harnessing sortase A transpeptidation for advanced targeted therapeutics and vaccine engineering. Biotechnology Advances 2023, 64 , 108108.
    24. Zhao Zipeng, Li Fangya, Zhang Jianyu. Deuterium Solvent Kinetic Isotope Effect on Enzymatic Methyl Transfer Catalyzed by Catechol O-methyltransferase. Protein & Peptide Letters 2023, 30 (4) , 351-359.
    25. Aaron D. Smith, Scott A. Walper, Igor L. Medintz. Enzymatic bioconjugation to nanoparticles. 2023, 337-368.
    26. Holly E. Morgan, W. Bruce Turnbull, Michael E. Webb. Challenges in the use of sortase and other peptide ligases for site-specific protein modification. Chemical Society Reviews 2022, 51 (10) , 4121-4145.
    27. Fabian Barthels, Jessica Meyr, Stefan J. Hammerschmidt, Tessa Marciniak, Hans-Joachim Räder, Wilma Ziebuhr, Bernd Engels, Tanja Schirmeister. 2-Sulfonylpyrimidines as Privileged Warheads for the Development of S. aureus Sortase A Inhibitors. Frontiers in Molecular Biosciences 2022, 8
    28. Dmitry A. Shulga, Konstantin V. Kudryavtsev. Selection of Promising Novel Fragment Sized S. aureus SrtA Noncovalent Inhibitors Based on QSAR and Docking Modeling Studies. Molecules 2021, 26 (24) , 7677.
    29. Aliyath Susmitha, Harsha Bajaj, Kesavan Madhavan Nampoothiri. The divergent roles of sortase in the biology of Gram-positive bacteria. The Cell Surface 2021, 7 , 100055.
    30. Ming-Hao Yang, Chung-Chi Hu, Chi-Hzeng Wong, Jian-Jong Liang, Hui-Ying Ko, Meng-Hsun He, Yi-Ling Lin, Na-Sheng Lin, Yau-Heiu Hsu. Convenient Auto-Processing Vector Based on Bamboo Mosaic Virus for Presentation of Antigens Through Enzymatic Coupling. Frontiers in Immunology 2021, 12
    31. Christine M. Artim, Manisha Kunala, Meghan K. O'Leary, Christopher A. Alabi. PEGylated Oligothioetheramide Prodrugs Activated by Host Serum Proteases. ChemBioChem 2021, 22 (17) , 2697-2702.
    32. Chandrabose Selvaraj, Gurudeeban Selvaraj, Randa Mohamed Ismail, Rajendran Vijayakumar, Alaa Baazeem, Dong-Qing Wei, Sanjeev Kumar Singh. Interrogation of Bacillus anthracis SrtA active site loop forming open/close lid conformations through extensive MD simulations for understanding binding selectivity of SrtA inhibitors. Saudi Journal of Biological Sciences 2021, 28 (7) , 3650-3659.
    33. Tongtong Tian, Jinzhi Zhao, Yuning Wang, Binxiao Li, Liang Qiao, Kun Zhang, Baohong Liu. Transpeptidation-mediated single-particle imaging assay for sensitive and specific detection of sortase with dark-field optical microscopy. Biosensors and Bioelectronics 2021, 178 , 113003.
    34. Adrian C. D. Fuchs, Moritz Ammelburg, Jörg Martin, Ruth A. Schmitz, Marcus D. Hartmann, Andrei N. Lupas. Archaeal Connectase is a specific and efficient protein ligase related to proteasome β subunits. Proceedings of the National Academy of Sciences 2021, 118 (11)
    35. Harry Morrison. Peptidyl arginine deiminase 4. 2021, 153-157.
    36. Jason E. Gosschalk, Chungyu Chang, Christopher K. Sue, Sara D. Siegel, Chenggang Wu, Michele D. Kattke, Sung Wook Yi, Robert Damoiseaux, Michael E. Jung, Hung Ton-That, Robert T. Clubb. A Cell-based Screen in Actinomyces oris to Identify Sortase Inhibitors. Scientific Reports 2020, 10 (1)
    37. Magdalena Wójcik, Kamil Szala, Ronald van Merkerk, Wim J. Quax, Ykelien L. Boersma. Engineering the specificity of Streptococcus pyogenes sortase A by loop grafting. Proteins: Structure, Function, and Bioinformatics 2020, 88 (11) , 1394-1400.
    38. Zhi Zou, Diana M. Mate, Maximilian Nöth, Felix Jakob, Ulrich Schwaneberg. Enhancing Robustness of Sortase A by Loop Engineering and Backbone Cyclization. Chemistry – A European Journal 2020, 26 (60) , 13568-13572.
    39. Min Woo Ha, Sung Wook Yi, Seung-Mann Paek. Design and Synthesis of Small Molecules as Potent Staphylococcus aureus Sortase A Inhibitors. Antibiotics 2020, 9 (10) , 706.
    40. Fabian Barthels, Gabriella Marincola, Tessa Marciniak, Matthias Konhäuser, Stefan Hammerschmidt, Jan Bierlmeier, Ute Distler, Peter R. Wich, Stefan Tenzer, Dirk Schwarzer, Wilma Ziebuhr, Tanja Schirmeister. Asymmetric Disulfanylbenzamides as Irreversible and Selective Inhibitors of Staphylococcus aureus Sortase A. ChemMedChem 2020, 15 (10) , 839-850.
    41. Chia-Yu Kang, I-Hsiu Huang, Chi-Chi Chou, Tsai-Yu Wu, Jyun-Cyuan Chang, Yu-Yuan Hsiao, Cheng-Hsuan Cheng, Wei-Jiun Tsai, Kai-Cheng Hsu, Shuying Wang. Functional analysis of Clostridium difficile sortase B reveals key residues for catalytic activity and substrate specificity. Journal of Biological Chemistry 2020, 295 (11) , 3734-3745.
    42. Aliyath Susmitha, Kesavan Madhavan Nampoothiri, Harsha Bajaj. Insights into the biochemical and functional characterization of sortase E transpeptidase of Corynebacterium glutamicum. Biochemical Journal 2019, 476 (24) , 3835-3847.
    43. Saima Younis, Sameera Taj, Sajid Rashid. Structural studies of Staphylococcus aureus Sortase inhibiton via Conus venom peptides. Archives of Biochemistry and Biophysics 2019, 671 , 87-102.
    44. Salvatore Di Girolamo, Chasper Puorger, Mara Castiglione, Maren Vogel, Rémy Gébleux, Manfred Briendl, Tamara Hell, Roger R. Beerli, Ulf Grawunder, Georg Lipps. Characterization of the housekeeping sortase from the human pathogen Propionibacterium acnes : first investigation of a class F sortase. Biochemical Journal 2019, 476 (4) , 665-682.
    45. Lina Bartels, Hidde L. Ploegh, Hergen Spits, Koen Wagner. Preparation of bispecific antibody-protein adducts by site-specific chemo-enzymatic conjugation. Methods 2019, 154 , 93-101.
    46. Magdalena Lukaszczyk, Brajabandhu Pradhan, Han Remaut. The Biosynthesis and Structures of Bacterial Pili. 2019, 369-413.
    47. Hejia Henry Wang, Andrew Tsourkas. Overcoming the Limitations of Sortase with Proximity-Based Sortase-Mediated Ligation (PBSL). 2019, 165-177.
    48. Hejia Henry Wang, Andrew Tsourkas. Site-Specific C-Terminal Labeling of Recombinant Proteins with Proximity-Based Sortase-Mediated Ligation (PBSL). 2019, 15-28.
    49. Xiao Wang, Jia-Liang Chen, Gottfried Otting, Xun-Cheng Su. Conversion of an amide to a high-energy thioester by Staphylococcus aureus sortase A is powered by variable binding affinity for calcium. Scientific Reports 2018, 8 (1)
    50. Ilke Ugur, Martin Schatte, Antoine Marion, Manuel Glaser, Mara Boenitz-Dulat, Iris Antes, . Ca2+ binding induced sequential allosteric activation of sortase A: An example for ion-triggered conformational selection. PLOS ONE 2018, 13 (10) , e0205057.
    51. Mohd Farid Abdul Halim, Ronald Rodriguez, Jonathan D. Stoltzfus, Iain G. Duggin, Mechthild Pohlschroder. Conserved residues are critical for Haloferax volcanii archaeosortase catalytic activity: Implications for convergent evolution of the catalytic mechanisms of non‐homologous sortases from archaea and bacteria. Molecular Microbiology 2018, 108 (3) , 276-287.
    52. Georg Falck, Kristian Müller. Enzyme-Based Labeling Strategies for Antibody–Drug Conjugates and Antibody Mimetics. Antibodies 2018, 7 (1) , 4.
    53. Joel Haywood, Jason W Schmidberger, Amy M James, Samuel G Nonis, Kirill V Sukhoverkov, Mikael Elias, Charles S Bond, Joshua S Mylne. Structural basis of ribosomal peptide macrocyclization in plants. eLife 2018, 7
    54. Amy M Weeks, James A Wells. Engineering peptide ligase specificity by proteomic identification of ligation sites. Nature Chemical Biology 2018, 14 (1) , 50-57.
    55. Eiji Tamai, Hiroshi Sekiya, Jun Maki, Hirofumi Nariya, Hiromi Yoshida, Shigehiro Kamitori. X-ray structure of Clostridium perfringens sortase B cysteine transpeptidase. Biochemical and Biophysical Research Communications 2017, 493 (3) , 1267-1272.
    56. Muna Suliman, Vishaka Santosh, Tom C. M. Seegar, Annamarie C. Dalton, Kathryn M. Schultz, Candice S. Klug, William A. Barton, . Directed evolution provides insight into conformational substrate sampling by SrtA. PLOS ONE 2017, 12 (8) , e0184271.
    57. Hejia Henry Wang, Burcin Altun, Kido Nwe, Andrew Tsourkas. Proximity‐Based Sortase‐Mediated Ligation. Angewandte Chemie 2017, 129 (19) , 5433-5436.
    58. Hejia Henry Wang, Burcin Altun, Kido Nwe, Andrew Tsourkas. Proximity‐Based Sortase‐Mediated Ligation. Angewandte Chemie International Edition 2017, 56 (19) , 5349-5352.
    59. Sreetama Das, Vijaykumar S. Pawale, Venkatareddy Dadireddy, Avinash Kumar Singh, Suryanarayanarao Ramakumar, Rajendra P. Roy. Structure and specificity of a new class of Ca2+-independent housekeeping sortase from Streptomyces avermitilis provide insights into its non-canonical substrate preference. Journal of Biological Chemistry 2017, 292 (17) , 7244-7257.
    60. Jian Gao, Zhongwu Guo. Synthetic Studies of GPI-Anchored Peptides, Glycopeptides, and Proteins. 2017, 253-281.
    61. Fuguang Chen, Fang Xie, Baoling Yang, Chengcheng Wang, Siguo Liu, Yueling Zhang, . Streptococcus suis sortase A is Ca2+ independent and is inhibited by acteoside, isoquercitrin and baicalin. PLOS ONE 2017, 12 (3) , e0173767.
    62. Alex W. Jacobitz, Michele D. Kattke, Jeff Wereszczynski, Robert T. Clubb. Sortase Transpeptidases: Structural Biology and Catalytic Mechanism. 2017, 223-264.
    63. Benjamin J. Willson, Katalin Kovács, Tom Wilding-Steele, Robert Markus, Klaus Winzer, Nigel P. Minton. Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824. Biotechnology for Biofuels 2016, 9 (1)
    64. Jui-Chieh Yin, Chun-Hsien Fei, Yen-Chen Lo, Yu-Yuan Hsiao, Jyun-Cyuan Chang, Jay C. Nix, Yuan-Yu Chang, Lee-Wei Yang, I-Hsiu Huang, Shuying Wang. Structural Insights into Substrate Recognition by Clostridium difficile Sortase. Frontiers in Cellular and Infection Microbiology 2016, 6
    65. Jia‐Liang Chen, Xiao Wang, Feng Yang, Chan Cao, Gottfried Otting, Xun‐Cheng Su. 3D Structure Determination of an Unstable Transient Enzyme Intermediate by Paramagnetic NMR Spectroscopy. Angewandte Chemie International Edition 2016, 55 (44) , 13744-13748.
    66. Jia‐Liang Chen, Xiao Wang, Feng Yang, Chan Cao, Gottfried Otting, Xun‐Cheng Su. 3D Structure Determination of an Unstable Transient Enzyme Intermediate by Paramagnetic NMR Spectroscopy. Angewandte Chemie 2016, 128 (44) , 13948-13952.
    67. Pooja Shrestha, Jeff Wereszczynski. Discerning the catalytic mechanism of Staphylococcus aureus sortase A with QM/MM free energy calculations. Journal of Molecular Graphics and Modelling 2016, 67 , 33-43.
    68. Elfriede Dall, Hans Brandstetter. Structure and function of legumain in health and disease. Biochimie 2016, 122 , 126-150.
    69. Albert H. Chan, Sung Wook Yi, Austen L. Terwilliger, Anthony W. Maresso, Michael E. Jung, Robert T. Clubb. Structure of the Bacillus anthracis Sortase A Enzyme Bound to Its Sorting Signal. Journal of Biological Chemistry 2015, 290 (42) , 25461-25474.
    70. Md Munan Shaik, Charlotte Lombardi, Daniel Maragno Trindade, Daphna Fenel, Guy Schoehn, Anne Marie Di Guilmi, Andréa Dessen. A Structural Snapshot of Type II Pilus Formation in Streptococcus pneumoniae. Journal of Biological Chemistry 2015, 290 (37) , 22581-22592.
    71. Heejae Kim, Ka‐Hei Siu, Maryam Raeeszadeh‐Sarmazdeh, Qing Sun, Qi Chen, Wilfred Chen. Bioengineering strategies to generate artificial protein complexes. Biotechnology and Bioengineering 2015, 112 (8) , 1495-1505.
    72. Lena Schmohl, Felix Roman Wagner, Michael Schümann, Eberhard Krause, Dirk Schwarzer. Semisynthesis and initial characterization of sortase A mutants containing selenocysteine and homocysteine. Bioorganic & Medicinal Chemistry 2015, 23 (12) , 2883-2889.
    73. William J. Bradshaw, Abigail H. Davies, Christopher J. Chambers, April K. Roberts, Clifford C. Shone, K. Ravi Acharya. Molecular features of the sortase enzyme family. FEBS Journal 2015, 282 (11) , 2097-2114.
    74. Werner Pansegrau, Fabio Bagnoli. Pilus Assembly in Gram-Positive Bacteria. 2015, 203-233.
    75. Elizabeth H Donahue, Lisa F Dawson, Esmeralda Valiente, Stuart Firth-Clark, Meriel R Major, Eddy Littler, Trevor R Perrior, Brendan W Wren. Clostridium difficilehas a single sortase, SrtB, that can be inhibited by small-molecule inhibitors. BMC Microbiology 2014, 14 (1)
    76. Hans C. van Leeuwen, Oleg I. Klychnikov, Mica A.C. Menks, Ed J. Kuijper, Jan W. Drijfhout, Paul J. Hensbergen. Clostridium difficile sortase recognizes a (S/P)PXTG sequence motif and can accommodate diaminopimelic acid as a substrate for transpeptidation. FEBS Letters 2014, 588 (23) , 4325-4333.
    77. Lena Schmohl, Dirk Schwarzer. Sortase-mediated ligations for the site-specific modification of proteins. Current Opinion in Chemical Biology 2014, 22 , 122-128.
    78. Markus Ritzefeld. Sortagging: A Robust and Efficient Chemoenzymatic Ligation Strategy. Chemistry – A European Journal 2014, 20 (28) , 8516-8529.
    79. Chandrabose Selvaraj, Jeyachandran Sivakamavalli, Vaseeharan Baskaralingam, Sanjeev Kumar Singh. Virtual screening of LPXTG competitive SrtA inhibitors targeting signal transduction mechanism in Bacillus anthracis : a combined experimental and theoretical study. Journal of Receptors and Signal Transduction 2014, 34 (3) , 221-232.
    80. David M. Shlaes, Lefa Alksne, Steven J. Projan. The Pharmaceutical Industry and Inhibitors of Bacterial Enzymes: Implications for Drug Development. 2014, 215-225.
    81. Alex W. Jacobitz, Jeff Wereszczynski, Sung Wook Yi, Brendan R. Amer, Grace L. Huang, Angelyn V. Nguyen, Michael R. Sawaya, Michael E. Jung, J.Andrew McCammon, Robert T. Clubb. Structural and Computational Studies of the Staphylococcus aureus Sortase B-Substrate Complex Reveal a Substrate-stabilized Oxyanion Hole. Journal of Biological Chemistry 2014, 289 (13) , 8891-8902.
    82. Christian Linke-Winnebeck, Neil G. Paterson, Paul G. Young, Martin J. Middleditch, David R. Greenwood, Gregor Witte, Edward N. Baker. Structural Model for Covalent Adhesion of the Streptococcus pyogenes Pilus through a Thioester Bond. Journal of Biological Chemistry 2014, 289 (1) , 177-189.
    83. Aimin Yang, Lei Zhao, Yao-Wen Wu. Chemical Synthesis and Biological Function of Lipidated Proteins. 2014, 137-182.
    84. Zheng Qu, Venkat Krishnamurthy, Carolyn A. Haller, Brent M. Dorr, Ulla M. Marzec, Sawan Hurst, Monica T. Hinds, Stephen R. Hanson, David R. Liu, Elliot L. Chaikof. Immobilization of Actively Thromboresistant Assemblies on Sterile Blood‐Contacting Surfaces. Advanced Healthcare Materials 2014, 3 (1) , 30-35.
    85. Dietmar Schomburg, Ida Schomburg. sortase A 2013, 98-121.
    86. László Polgár. Catalytic Mechanisms of Cysteine Peptidases. 2013, 1773-1784.
    87. Dewey G. McCafferty, Jeffrey A. Melvin. Sortases. 2013, 2459-2465.
    88. Xinyu Zhao, Guoshun Li, Shufang Liang. Several Affinity Tags Commonly Used in Chromatographic Purification. Journal of Analytical Methods in Chemistry 2013, 2013 , 1-8.
    89. Kalli Kappel, Jeff Wereszczynski, Robert T. Clubb, J. Andrew McCammon. The binding mechanism, multiple binding modes, and allosteric regulation of Staphylococcus aureus Sortase A probed by molecular dynamics simulations. Protein Science 2012, 21 (12) , 1858-1871.
    90. Thomas Spirig, Ethan M. Weiner, Robert T. Clubb. Sortase enzymes in Gram‐positive bacteria. Molecular Microbiology 2011, 82 (5) , 1044-1059.
    91. Maximilian Wei‐Lin Popp, Hidde L. Ploegh. Making and Breaking Peptide Bonds: Protein Engineering Using Sortase. Angewandte Chemie International Edition 2011, 50 (22) , 5024-5032.
    92. Maximilian Wei‐Lin Popp, Hidde L. Ploegh. Bilden und Brechen von Peptidbindungen: Protein‐Engineering mithilfe von Sortase. Angewandte Chemie 2011, 123 (22) , 5128-5137.
    93. Boxue Tian, Leif A. Eriksson. Structural changes of Listeria monocytogenes sortase A: A key to understanding the catalytic mechanism. Proteins: Structure, Function, and Bioinformatics 2011, 79 (5) , 1564-1572.
    94. Yuichi Yamamura, Hidehiko Hirakawa, Satoshi Yamaguchi, Teruyuki Nagamune. Enhancement of sortase A-mediated protein ligation by inducing a β-hairpin structure around the ligation site. Chemical Communications 2011, 47 (16) , 4742.
    95. Ethan M. Weiner, Scott Robson, Melanie Marohn, Robert T. Clubb. The Sortase A Enzyme That Attaches Proteins to the Cell Wall of Bacillus anthracis Contains an Unusual Active Site Architecture. Journal of Biological Chemistry 2010, 285 (30) , 23433-23443.
    96. . References. 2010, 807-843.
    97. Kathleen W. Clancy, Jeffrey A. Melvin, Dewey G. McCafferty. Sortase transpeptidases: Insights into mechanism, substrate specificity, and inhibition. Peptide Science 2010, 94 (4) , 385-396.
    98. Nuttee Suree, Chu Kong Liew, Valerie A. Villareal, William Thieu, Evgeny A. Fadeev, Jeremy J. Clemens, Michael E. Jung, Robert T. Clubb. The Structure of the Staphylococcus aureus Sortase-Substrate Complex Reveals How the Universally Conserved LPXTG Sorting Signal Is Recognized. Journal of Biological Chemistry 2009, 284 (36) , 24465-24477.
    99. Konstantin V. Kudryavtsev, Matthew L. Bentley, Dewey G. McCafferty. Probing of the cis-5-phenyl proline scaffold as a platform for the synthesis of mechanism-based inhibitors of the Staphylococcus aureus sortase SrtA isoform. Bioorganic & Medicinal Chemistry 2009, 17 (7) , 2886-2893.
    100. . Synthesis Concepts for Peptides and Proteins. 2009, 317-364.
    Load all citations

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    You’ve supercharged your research process with ACS and Mendeley!

    STEP 1:
    Click to create an ACS ID

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Your Mendeley pairing has expired. Please reconnect