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Rhodopsin Forms a Dimer with Cytoplasmic Helix 8 Contacts in Native Membranes

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Laboratory of Molecular Biology and Biochemistry, The Rockefeller University, 1230 York Avenue, New York, New York 10065, United States
Biomolecular Sciences and Biotechnology Institute and Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 7, 9747 AG Groningen, The Netherlands
*E-mail: [email protected]. Phone: (212) 327-8284.
Cite this: Biochemistry 2012, 51, 9, 1819–1821
Publication Date (Web):February 18, 2012
Copyright © 2012 American Chemical Society

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    G protein-coupled receptors form dimers and higher-order oligomers in membranes, but the precise mode of receptor–receptor interaction remains unknown. To probe the intradimeric proximity of helix 8 (H8), we conducted chemical cross-linking of endogenous cysteines in rhodopsin in disk membranes. We identified a Cys316–Cys316 cross-link using partial proteolysis and liquid chromatography with mass spectrometry. These results show that a symmetric dimer interface mediated by H1 and H8 contacts is present in native membranes.

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    Complete materials and methods, additional cross-linking gels, and mass spectrometry data. This material is available free of charge via the Internet at

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