ACS Publications. Most Trusted. Most Cited. Most Read
My Activity

Structure of the Bradykinin B2 Receptors' Amino Terminus

View Author Information
Institute of Physiological Chemistry and Pathobiochemsitry, the University at Mainz, Duesbergweg 6, 55099 Mainz, Germany, Institute of Molecular Biotechnology e. V., Beutenbergstrasse 11, 07745 Jena, Germany, Children's Hospital, Department of Clinical Chemistry and Biochemistry, University of Munich, Lindwurmstrasse 4, 80337, Germany, and Institute of Pharmacology and Toxicology, the University at Würzburg, Versbacherstrasse 9, 97078 Würzburg, Germany
Cite this: Biochemistry 1996, 35, 23, 7514–7519
Publication Date (Web):June 11, 1996
Copyright © 1996 American Chemical Society

    Article Views





    Read OnlinePDF (491 KB)


    The peptide hormone bradykinin exerts important biological functions by binding to and activating bradykinin B2 receptors. B2 receptors belong to the seven transmembrane domain (7TM) receptor family. Cloning of the cDNA sequences for the rat, human, and mouse bradykinin B2 receptor revealed several in-frame AUG triplets as potential initiation sites for translation. Due to “Kozak-like” consensus nucleotides, the AUG codon closest to transmembrane domain 1 was assumed the preferred initiation site for translation, but the real amino terminus of the B2 receptor protein was unknown. The amino terminus of several 7TM receptors has been shown to be essentially involved in receptor activation and/or ligand binding. Therefore we determined the amino terminus of the human and of the rat B2 receptor using domain-specific antipeptide antibodies, amino acid sequence analysis, and in vitro transcription/translation. We report that the human and rat B2 receptor protein start with the methionine which is translated from the first in-frame AUG. This start site extends the known amino terminus of the human and rat B2 receptors by 27 or 30 amino acid residues, respectively. Antibodies raised against a peptide of the initial 27 amino acids of the human B2 receptor stained B2 receptors on intact cells. This finding excludes the existence of a signal sequence for this receptor.

     This work was supported in part by the Deutsche Forschungsgemeinschaft Grant Mu 598/3-2.

     University at Mainz.


     Institute of Molecular Biotechnology.

     University of Munich.


     Address correspondence to this author at Institute of Pharmacology and Toxicology, the University at Würzburg, Versbacherstrasse 9, 97078 Würzburg, Germany. Phone:  0049-931-201-3982. Fax:  0049-931-201-3539.


     University of Würzburg.

     Abstract published in Advance ACS Abstracts, May 15, 1996.

    Cited By

    This article is cited by 22 publications.

    1. Sayo Yashima, Ayaka Shimazaki, Junya Mitoma, Tetsuto Nakagawa, Maya Abe, Hiroyuki Yamada, Hideyoshi Higashi. Close association of B2 bradykinin receptors with P2Y 2 ATP receptors. Journal of Biochemistry 2015, 158 (2) , 155-163.
    2. Regina Nostramo, Andrej Tillinger, Lidia Serova, Richard Kvetnansky, Esther L. Sabban. Bradykinin B2 Receptor in the Adrenal Medulla of Male Rats and Mice: Glucocorticoid-Dependent Increase With Immobilization Stress. Endocrinology 2013, 154 (10) , 3729-3738.
    3. L. M. Fredrik Leeb-Lundberg, . 3 Kinin receptor signaling and regulation. 2011
    4. Domenico Regoli, Francois Marceau. B-2 Bradykinin Receptor. 2007, 1-14.
    5. L. M. Fredrik Leeb-Lundberg, Francois Marceau, Werner Müller-Esterl, Douglas J. Pettibone, Bruce L. Zuraw. International Union of Pharmacology. XLV. Classification of the Kinin Receptor Family: from Molecular Mechanisms to Pathophysiological Consequences. Pharmacological Reviews 2005, 57 (1) , 27-77.
    6. Paola Cucchi, Stefania Meini, Laura Quartara, Alessandro Giolitti, Sabrina Zappitelli, Luigi Rotondaro, Carlo Alberto Maggi. Interaction of linear and cyclic peptide antagonists at the human B2 kinin receptor. Peptides 2002, 23 (8) , 1457-1463.
    7. Atsushi Kimura, Takahiro Kihara, Ryuichi Ohkura, Katsueki Ogiwara, Takayuki Takahashi. Localization of Bradykinin B2 Receptor in the Follicles of Porcine Ovary and Increased Expression of Matrix Metalloproteinase-3 and -20 in Cultured Granulosa Cells by Bradykinin Treatment1. Biology of Reproduction 2001, 65 (5) , 1462-1470.
    8. Christophe Eckard, Chiara Cabrele, Heike Wieland, Annette Beck-Sickinger. Characterisation of Neuropeptide Y Receptor Subtypes by Synthetic NPY Analogues and by Anti-receptor Antibodies. Molecules 2001, 6 (5) , 448-467.
    9. J. Fred Hess, Patricia J. Hey, Tsing-Bau Chen, Julie OBrien, Stacey S. Omalley, Douglas J. Pettibone, Raymond S. L. Chang. Molecular Cloning and Pharmacological Characterization of the Canine B1 and B2 Bradykinin Receptors. Biological Chemistry 2001, 382 (1) , 123-129.
    10. Jasminka Godovac-Zimmermann, Larry R. Brown. Perspectives for mass spectrometry and functional proteomics. Mass Spectrometry Reviews 2001, 20 (1) , 1-57.<1::AID-MAS1001>3.0.CO;2-J
    11. R. Couture, C.J. Lindsey. Chapter VII Brain kallikrein–kinin system: from receptors to neuronal pathways and physiological functions. 2000, 241-300.
    12. Said AbdAlla, Essam Zaki, Heinz Lother, Ursula Quitterer. Involvement of the Amino Terminus of the B2 Receptor in Agonist-induced Receptor Dimerization. Journal of Biological Chemistry 1999, 274 (37) , 26079-26084.
    13. Ursula Quitterer, Essam Zaki, Said AbdAlla. Investigation of the Extracellular Accessibility of the Connecting Loop between Membrane Domains I and II of the Bradykinin B2 Receptor. Journal of Biological Chemistry 1999, 274 (21) , 14773-14778.
    14. Vukic Soskic, Elke Nyakatura, Martin Roos, Werner Müller-Esterl, Jasminka Godovac-Zimmermann. Correlations in Palmitoylation and Multiple Phosphorylation of Rat Bradykinin B2 Receptor in Chinese Hamster Ovary Cells. Journal of Biological Chemistry 1999, 274 (13) , 8539-8545.
    15. Christophe P. Eckard, Annette G. Beck-Sickinger, Heike A. Wieland. Comparison of Antibodies Directed Against Receptor Segments of NPY-Receptors. Journal of Receptors and Signal Transduction 1999, 19 (1-4) , 379-394.
    16. Jeanette Erdmann, Nina Hegemann, Alexander Weidemann, Heike Kallisch, Manfred Hummel, Roland Hetzer, Eckart Fleck, Vera Regitz-Zagrosek. Screening the human bradykinin B2 receptor gene in patients with cardiovascular diseases: Identification of a functional mutation in the promoter and a new coding variant (T21M). American Journal of Medical Genetics 1998, 80 (5) , 521-525.<521::AID-AJMG17>3.0.CO;2-2
    17. J.B. Pesquero, M. Bader. Molecular biology of the kallikrein-kinin system: from structure to function. Brazilian Journal of Medical and Biological Research 1998, 31 (9) , 1197-1203.
    18. Stephen G Farmer, Steve J Powell, Deidre E Wilkins, Alexander Graham. Cloning, sequencing and functional expression of a guinea pig lung bradykinin B2 receptor. European Journal of Pharmacology 1998, 346 (2-3) , 291-298.
    19. Werner Müller-Esterl. Immunological Probes for the Bradykinin B2 Receptor. A Toolbox. 1997, 99-110.
    20. Andree Blaukat, Said Abd Alla, Martin J. Lohse, Werner Müller-Esterl. Ligand-induced Phosphorylation/Dephosphorylation of the Endogenous Bradykinin B2 Receptor from Human Fibroblasts. Journal of Biological Chemistry 1996, 271 (50) , 32366-32374.
    21. Said AbdAlla, Kurt Jarnagin, Werner Müller-Esterl, Ursula Quitterer. The N-terminal Amino Group of [Tyr8]Bradykinin Is Bound Adjacent to Analogous Amino Acids of the Human and Rat B2 Receptor. Journal of Biological Chemistry 1996, 271 (44) , 27382-27387.
    22. Said Abd Alla, Ursula Quitterer, Christian Schröder, Andree Blaukat, Angelika Horstmeyer, Jürgen Dedio, Helmut Reiländer, Werner Müller-Esterl. On the structure of the amino-terminal domain ED1 of the B2 receptor. Immunopharmacology 1996, 35 (1) , 23-26.

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    You’ve supercharged your research process with ACS and Mendeley!

    STEP 1:
    Click to create an ACS ID

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Your Mendeley pairing has expired. Please reconnect