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    Uncovering a Calcium-Regulated Membrane-Binding Mechanism for Soybean Lipoxygenase-1†
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    Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, P.O. Box 10011, Charlottesville, Virginia 22906-0011, and Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, 30239 Krakow, Poland
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    Biochemistry

    Cite this: Biochemistry 1998, 37, 44, 15481–15490
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    https://doi.org/10.1021/bi981062t
    Published October 16, 1998
    Copyright © 1998 American Chemical Society
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    Abstract

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    Lipoxygenases catalyze the biosynthesis of leukotrienes, lipoxins, and other lipid-derived mediators that are involved in a wide variety of pathophysiological processes, including inflammation, allergy, and tumorigenesis. Mammalian lipoxygenases are activated by a calcium-mediated translocation to intracellular membranes upon cell stimulation, and cooperate with cytosolic phospholipase A2 at the membrane surface to generate eicosanoids. Although it has been documented that plant cell stimulation increases intracellular Ca2+ concentration and activates cytosolic phospholipase A2, followed by lipoxygenase-catalyzed conversion of the liberated linolenic acid to jasmonic acid, no evidence is available for Ca2+-regulated membrane binding and activity of plant lipoxygenases. Plant lipoxygenases, unlike their mammalian counterparts, are believed to function independently of calcium or membranes. Here we present spectroscopic evidence for a calcium-regulated membrane-binding mechanism of soybean lipoxygenase-1 (L-1). Both calcium and membrane binding affect the structure and the mode of action of L-1. Free L-1 in solution is less accessible to the polar solvent and converts linoleic acid to conjugated dienes, whereas surface binding increases solvent accessibility and stimulates conjugated ketodiene production. Calcium exerts a biphasic effect on the structure and activity of L-1. Our results uncover a new regulatory mechanism for plant lipoxygenases and delineate common features in animal and plant cell signaling pathways.

    Copyright © 1998 American Chemical Society

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    †

     Supported in part by a grant from American Heart Association to S.A.T. (96−1364). J.S. was supported in part by an International Research Scholarship award from the Howard Hughes Medical Institute (HHMI 77195-543901).

    *

     Corresponding author. Telephone:  (804) 982-3855. Fax:  (804) 982-1616. E-mail:  [email protected].

    ‡

     University of Virginia School of Medicine.

    §

     Polish Academy of Sciences.

    ‖

     Present address:  HemoCleanse Inc., Purdue Research Park, West Lafayette, Indiana 47906.

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    Biochemistry

    Cite this: Biochemistry 1998, 37, 44, 15481–15490
    Click to copy citationCitation copied!
    https://doi.org/10.1021/bi981062t
    Published October 16, 1998
    Copyright © 1998 American Chemical Society
    Request reuse permissions

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