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Enzymatically Cross-Linked Hyperbranched Polyglycerol Hydrogels as Scaffolds for Living Cells

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† Institut für Chemie und Biochemie, Freie Universität Berlin, Takustraße 3, 14195 Berlin, Germany
‡ Institut für Chemie, Technische Universität Berlin, Straße des 17. Juni 124, 10623 Berlin, Germany
⊥ Institut für Laboratoriumsmedizin, Klinische Chemie und Pathobiochemie, Charité - Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany
§ Institut für Mikrobiologie, Technische Universität Dresden, 01062 Dresden, Germany
*E-mail: [email protected]. Fax: +49-30-838 53357.
Cite this: Biomacromolecules 2014, 15, 11, 3881–3890
Publication Date (Web):August 21, 2014
https://doi.org/10.1021/bm500705x
Copyright © 2014 American Chemical Society
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    Abstract

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    Although several strategies are now available to enzymatically cross-link linear polymers to hydrogels for biomedical use, little progress has been reported on the use of dendritic polymers for the same purpose. Herein, we demonstrate that horseradish peroxidase (HRP) successfully catalyzes the oxidative cross-linking of a hyperbranched polyglycerol (hPG) functionalized with phenol groups to hydrogels. The tunable cross-linking results in adjustable hydrogel properties. Because the obtained materials are cytocompatible, they have great potential for encapsulating living cells for regenerative therapy. The gel formation can be triggered by glucose and controlled well under various environmental conditions.

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    Characterizations and synthesis of hPG-HPA, hPG-TG, hPG-HBA, and hPG-HPA-NAP (compound 1, 2, 3, 4 in Table 1); rheological study; cytotoxicity of compounds and enzymes used; H2O2 content determination; cell adhesion on/in hydrogels incorporated with fibronectin; glucose-triggered hPG-HPA gel formation. This material is available free of charge via the Internet at http://pubs.acs.org.

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