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Adsorption Force of Fibronectin on Various Surface Chemistries and Its Vital Role in Osteoblast Adhesion

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Key Laboratory of Biorheological Science and Technology, Ministry of Education, and Research Center of Bioinspired Materials Science and Engineering, College of Bioengineering, Chongqing University, Chongqing, 400030, China
§ Center for Human Tissue and Organs Degeneration, Institute Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China
*E-mail: [email protected]. Fax: +86 23 65102507.
Cite this: Biomacromolecules 2015, 16, 3, 973–984
Publication Date (Web):February 16, 2015
https://doi.org/10.1021/bm501873g
Copyright © 2015 American Chemical Society
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Abstract

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The amount, type, and conformation of proteins adsorbed on an implanted biomaterial are believed to influence cell adhesion. Nevertheless, only a few research works have been dedicated to the contribution of protein adsorption force. To verify our hypothesis that the adsorption force of protein on biomaterial is another crucial mediator to cell adhesion, fibronectin (FN) adsorbed on self-assembled monolayers (SAMs) with terminal −OH, −CH3, and −NH2 was quantified for FN adsorption force (Fad) by utilizing a sphere/plane adsorption model and parallel plate flow chamber. As revealed, Fad on SAMs followed a chemistry-dependence of −NH2 > −CH3 ≫ −OH. It is further demonstrated that Fad together with FN conformation could regulate the late osteoblast adhesion and the consequent reorganization of the adsorbed FN and fibrillogenesis of the endogenous FN. Our study suggests that protein adsorption force plays a key role in cell adhesion and should be involved for better biomaterial design.

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XPS characterizations of the Au–SAMs (Figure S1) and Glass–SAMs (Figure S2). Representative images of the detachment process of FN conjugated microspheres under laminar shear stress (Figure S3). Size distribution of FBs and FAs on various SAMs (Figure S4). Calculation of the fibronectin adsorption force per FN monomer in Table 2. This material is available free of charge via the Internet at http://pubs.acs.org.

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