Adsorption Force of Fibronectin on Various Surface Chemistries and Its Vital Role in Osteoblast Adhesion
- Manping Lin ,
- Huaiyu Wang ,
- Changshun Ruan ,
- Juan Xing ,
- Jinfeng Wang ,
- Yan Li ,
- Yuanliang Wang , and
- Yanfeng Luo
Abstract

The amount, type, and conformation of proteins adsorbed on an implanted biomaterial are believed to influence cell adhesion. Nevertheless, only a few research works have been dedicated to the contribution of protein adsorption force. To verify our hypothesis that the adsorption force of protein on biomaterial is another crucial mediator to cell adhesion, fibronectin (FN) adsorbed on self-assembled monolayers (SAMs) with terminal −OH, −CH3, and −NH2 was quantified for FN adsorption force (Fad) by utilizing a sphere/plane adsorption model and parallel plate flow chamber. As revealed, Fad on SAMs followed a chemistry-dependence of −NH2 > −CH3 ≫ −OH. It is further demonstrated that Fad together with FN conformation could regulate the late osteoblast adhesion and the consequent reorganization of the adsorbed FN and fibrillogenesis of the endogenous FN. Our study suggests that protein adsorption force plays a key role in cell adhesion and should be involved for better biomaterial design.
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- Tianjie Li, Lijing Hao, Jiangyu Li, Chang Du, Yingjun Wang. Role of Ninth Type-III Domain of Fibronectin in the Mediation of Cell-Binding Domain Adsorption on Surfaces with Different Chemistries. Langmuir 2018, 34 (33) , 9847-9855. https://doi.org/10.1021/acs.langmuir.8b01937
- Tanja D. Becke, Stefan Ness, Raimund Gürster, Arndt F. Schilling, Anne-Marie di Guilmi, Stefanie Sudhop, Markus Hilleringmann, Hauke Clausen-Schaumann. Single Molecule Force Spectroscopy Reveals Two-Domain Binding Mode of Pilus-1 Tip Protein RrgA of Streptococcus pneumoniae to Fibronectin. ACS Nano 2018, 12 (1) , 549-558. https://doi.org/10.1021/acsnano.7b07247
- Daniela Serien and Shoji Takeuchi . Multi-Component Microscaffold With 3D Spatially Defined Proteinaceous Environment. ACS Biomaterials Science & Engineering 2017, 3 (3) , 487-494. https://doi.org/10.1021/acsbiomaterials.6b00695
- Ana R. Araújo, Diana Soares da Costa, Sara Amorim, Rui L. Reis, Ricardo A. Pires, and Iva Pashkuleva . Surfaces Mimicking Glycosaminoglycans Trigger Different Response of Stem Cells via Distinct Fibronectin Adsorption and Reorganization. ACS Applied Materials & Interfaces 2016, 8 (42) , 28428-28436. https://doi.org/10.1021/acsami.6b04472
- Pingping Ma, Yonglin Yu, Kendrick Hii Ru Yie, Kai Fang, Zixin Zhou, Xiaoyi Pan, Zhennan Deng, Xinkun Shen, Jinsong Liu. Effects of titanium with different micro/nano structures on the ability of osteoblasts to resist oxidative stress. Materials Science and Engineering: C 2021, 123 , 111969. https://doi.org/10.1016/j.msec.2021.111969
- Ruriko Sekiya-Aoyama, Yoshinori Arisaka, Masahiro Hakariya, Hiroki Masuda, Takanori Iwata, Tetsuya Yoda, Nobuhiko Yui. Dual effect of molecular mobility and functional groups of polyrotaxane surfaces on the fate of mesenchymal stem cells. Biomaterials Science 2021, 9 (3) , 675-684. https://doi.org/10.1039/D0BM01782E
- Soichiro Deguchi, Ryo Yokoyama, Takuya Maki, Kazuki Tomita, Ryosuke Osugi, Masataka Hakamada, Mamoru Mabuchi. A new mechanism for reduced cell adhesion: Adsorption dynamics of collagen on a nanoporous gold surface. Materials Science and Engineering: C 2021, 119 , 111461. https://doi.org/10.1016/j.msec.2020.111461
- Yi-Fan Chen, Clyde Goodheart, Diego Rua. The Body’s Cellular and Molecular Response to Protein-Coated Medical Device Implants: A Review Focused on Fibronectin and BMP Proteins. International Journal of Molecular Sciences 2020, 21 (22) , 8853. https://doi.org/10.3390/ijms21228853
- Kazuhiko Ishihara, Katsuya Mitera, Yuuki Inoue, Kyoko Fukazawa. Effects of molecular interactions at various polymer brush surfaces on fibronectin adsorption induced cell adhesion. Colloids and Surfaces B: Biointerfaces 2020, 194 , 111205. https://doi.org/10.1016/j.colsurfb.2020.111205
- Wanqi Yu, Xiaona Wang, Yu Guo, Shihui Yang, Zhe Zhou, Xiaolong Sun, Ruwen Zhang, Tianqi Guo, Yanmin Zhou, Jinghui Zhao. The osteogenesis performance of titanium modified via plasma-enhanced chemical vapor deposition: in vitro and in vivo studies. Biomedical Materials 2020, 15 (5) , 055012. https://doi.org/10.1088/1748-605X/ab9096
- Maryam Rahmati, Eduardo A. Silva, Janne E. Reseland, Catherine A. Heyward, Håvard J. Haugen. Biological responses to physicochemical properties of biomaterial surface. Chemical Society Reviews 2020, 49 (15) , 5178-5224. https://doi.org/10.1039/D0CS00103A
- Maura Cimino, Paula Parreira, Sílvia J. Bidarra, Raquel M. Gonçalves, Cristina C. Barrias, M. Cristina L. Martins. Effect of surface chemistry on hMSC growth under xeno-free conditions. Colloids and Surfaces B: Biointerfaces 2020, 189 , 110836. https://doi.org/10.1016/j.colsurfb.2020.110836
- Monika Golda-Cepa, Klas Engvall, Minna Hakkarainen, Andrzej Kotarba. Recent progress on parylene C polymer for biomedical applications: A review. Progress in Organic Coatings 2020, 140 , 105493. https://doi.org/10.1016/j.porgcoat.2019.105493
- Soichiro Deguchi, Masataka Hakamada, Jumpei Shingu, Susumu Sakakibara, Hironobu Sugiyama, Mamoru Mabuchi. Inactivation of HeLa cells on nanoporous gold. Materialia 2019, 7 , 100370. https://doi.org/10.1016/j.mtla.2019.100370
- Jie Cheng, Feng Geng, Jun Hu, Junhong Lü. Single-molecule measurement and bioinformatics analysis suggest a preferred orientation of human coagulation factor VIII on hydrophobic interfaces. Biophysical Chemistry 2019, 248 , 9-15. https://doi.org/10.1016/j.bpc.2019.03.001
- Juan Xing, Xianchao Pan, Hui Zhang, Jinfeng Wang, Yufei Ma, Yuanliang Wang, Yanfeng Luo. Shape recovery strain and nanostructures on recovered polyurethane films and their regulation to osteoblasts morphology. Journal of the Mechanical Behavior of Biomedical Materials 2019, 92 , 128-136. https://doi.org/10.1016/j.jmbbm.2019.01.008
- Xudong Shi, Liguo Cui, Hai Sun, Ni Jiang, Liping Heng, Xiuli Zhuang, Zhihua Gan, Xuesi Chen. Promoting cell growth on porous PLA microspheres through simple degradation methods. Polymer Degradation and Stability 2019, 161 , 319-325. https://doi.org/10.1016/j.polymdegradstab.2019.01.003
- Zifeng Lin, Mingming Wu, Huimin He, Qingfei Liang, Chengshen Hu, Zhiwen Zeng, Delin Cheng, Guocheng Wang, Dafu Chen, Haobo Pan, Changshun Ruan. 3D Printing of Mechanically Stable Calcium-Free Alginate-Based Scaffolds with Tunable Surface Charge to Enable Cell Adhesion and Facile Biofunctionalization. Advanced Functional Materials 2019, 29 (9) , 1808439. https://doi.org/10.1002/adfm.201808439
- Noreen J. Hickok, Edward T.J. Rochford, David J. Jaekel, R. Geoff Richards, T. Fintan Moriarty, Alexandra H.C. Poulsson. Bacterial Interactions With PEEK. 2019,,, 121-145. https://doi.org/10.1016/B978-0-12-812524-3.00009-0
- Deliang Zeng, Xingdi Zhang, Xiao Wang, Qingfeng Huang, Jin Wen, Xinchao Miao, Lingjie Peng, Yongsheng Li, Xinquan Jiang. The osteoimmunomodulatory properties of MBG scaffold coated with amino functional groups. Artificial Cells, Nanomedicine, and Biotechnology 2018, 46 (7) , 1425-1435. https://doi.org/10.1080/21691401.2017.1369428
- Lin Lv, Youtao Xie, Kai Li, Tao Hu, Xiang Lu, Yunzhen Cao, Xuebin Zheng. Unveiling the Mechanism of Surface Hydrophilicity‐Modulated Macrophage Polarization. Advanced Healthcare Materials 2018, 7 (19) , 1800675. https://doi.org/10.1002/adhm.201800675
- Cristina González-García, Marco Cantini, José Ballester-Beltrán, George Altankov, Manuel Salmerón-Sánchez. The strength of the protein-material interaction determines cell fate. Acta Biomaterialia 2018, 77 , 74-84. https://doi.org/10.1016/j.actbio.2018.07.016
- Rui Chen, John A. Hunt, Sandra Fawcett, Raechelle D'sa, Riaz Akhtar, Judith M. Curran. The optimization and production of stable homogeneous amine enriched surfaces with characterized nanotopographical properties for enhanced osteoinduction of mesenchymal stem cells. Journal of Biomedical Materials Research Part A 2018, 106 (7) , 1862-1877. https://doi.org/10.1002/jbm.a.36383
- Manping Lin, Shilong Mao, Jinfeng Wang, Juan Xing, Yuanliang Wang, Kaiyong Cai, Yanfeng Luo. Adsorption force of fibronectin controls transmission of cell traction force and subsequent stem cell fate. Biomaterials 2018, 162 , 170-182. https://doi.org/10.1016/j.biomaterials.2018.01.036
- Changshun Ruan, Nan Hu, Yufei Ma, Yuxiao Li, Juan Liu, Xinzhou Zhang, Haobo Pan. The interfacial pH of acidic degradable polymeric biomaterials and its effects on osteoblast behavior. Scientific Reports 2017, 7 (1) https://doi.org/10.1038/s41598-017-06354-1
- Lin Lv, Kai Li, Youtao Xie, Yunzhen Cao, Xuebin Zheng. Enhanced osteogenic activity of anatase TiO 2 film: Surface hydroxyl groups induce conformational changes in fibronectin. Materials Science and Engineering: C 2017, 78 , 96-104. https://doi.org/10.1016/j.msec.2017.04.056
- Young Jung No, Seyediman Roohaniesfahani, Zufu Lu, Jeffrey Shi, Hala Zreiqat. Strontium-doped calcium silicate bioceramic with enhanced in vitro osteogenic properties. Biomedical Materials 2017, 12 (3) , 035003. https://doi.org/10.1088/1748-605X/aa6987
- Zehui Liu, Yizhou Zhu, Xiangmei Liu, K.W.K. Yeung, Shuilin Wu. Construction of poly (vinyl alcohol)/poly (lactide-glycolide acid)/vancomycin nanoparticles on titanium for enhancing the surface self-antibacterial activity and cytocompatibility. Colloids and Surfaces B: Biointerfaces 2017, 151 , 165-177. https://doi.org/10.1016/j.colsurfb.2016.12.016
- Yanfeng Luo, Juan Xing, Manping Lin. The Biocompatibility of the Scaffolds Reinforced by Fibers or Tubes for Tissue Repair. 2017,,, 145-177. https://doi.org/10.1007/978-981-10-3554-8_5
- Haoyu Wang, Pinar Akcora. Confinement effect on the structure and elasticity of proteins interfacing polymers. Soft Matter 2017, 13 (8) , 1561-1568. https://doi.org/10.1039/C6SM02179D
- Yan Li, Jinfeng Wang, Juan Xing, Yuanliang Wang, Yanfeng Luo. Surface chemistry regulates the sensitivity and tolerability of osteoblasts to various magnitudes of fluid shear stress. Journal of Biomedical Materials Research Part A 2016, 104 (12) , 2978-2991. https://doi.org/10.1002/jbm.a.35848
- Juan Xing, Yufei Ma, Manping Lin, Yuanliang Wang, Haobo Pan, Changshun Ruan, Yanfeng Luo. Stretching-induced nanostructures on shape memory polyurethane films and their regulation to osteoblasts morphology. Colloids and Surfaces B: Biointerfaces 2016, 146 , 431-441. https://doi.org/10.1016/j.colsurfb.2016.06.044
- John D Krutty, Samantha K Schmitt, Padma Gopalan, William L Murphy. Surface functionalization and dynamics of polymeric cell culture substrates. Current Opinion in Biotechnology 2016, 40 , 164-169. https://doi.org/10.1016/j.copbio.2016.05.006
- Felix Jakob Segerer, Peter Johan Friedrich Röttgermann, Simon Schuster, Alicia Piera Alberola, Stefan Zahler, Joachim Oskar Rädler. Versatile method to generate multiple types of micropatterns. Biointerphases 2016, 11 (1) , 011005. https://doi.org/10.1116/1.4940703
- Rajendra Aithal, Amber Doss, Deepak Kumaraswamy, David Mills, Debasish Kuila. Growth and Functionality of Cells Cultured on Conducting and Semi-Conducting Surfaces Modified with Self-Assembled Monolayers (SAMs). Coatings 2016, 6 (1) , 9. https://doi.org/10.3390/coatings6010009
- Zhenming Wang, Kefeng Wang, Yanning Zhang, Yanan Jiang, Xiong Lu, Liming Fang, Donglin Gan, Chen Lv, Hongping Zhang, Shuxin Qu. Protein-Affinitive Polydopamine Nanoparticles as an Efficient Surface Modification Strategy for Versatile Porous Scaffolds Enhancing Tissue Regeneration. Particle & Particle Systems Characterization 2016, 33 (2) , 89-100. https://doi.org/10.1002/ppsc.201500187
- Keiichiro Kushiro, Chih-Hao Lee, Madoka Takai. Simultaneous characterization of protein–material and cell–protein interactions using dynamic QCM-D analysis on SAM surfaces. Biomaterials Science 2016, 4 (6) , 989-997. https://doi.org/10.1039/C5BM00613A
- Yusuke Arima, Hiroo Iwata. Preferential adsorption of cell adhesive proteins from complex media on self-assembled monolayers and its effect on subsequent cell adhesion. Acta Biomaterialia 2015, 26 , 72-81. https://doi.org/10.1016/j.actbio.2015.08.033



