Identification of Synthetic Steroids in River Water Downstream from Pharmaceutical Manufacture Discharges Based on a Bioanalytical Approach and Passive Sampling
- Nicolas Creusot
- ,
- Selim Aït-Aïssa
- ,
- Nathalie Tapie
- ,
- Patrick Pardon
- ,
- François Brion
- ,
- Wilfried Sanchez
- ,
- Eric Thybaud
- ,
- Jean-Marc Porcher
- , and
- Hélène Budzinski
Abstract

A bioanalytical approach was used to identify chemical contaminants at river sites located downstream from a pharmaceutical factory, where reproductive alterations in wild fish have been previously observed. By using polar organic compound integrative samplers (POCIS) at upstream and downstream sites, biological activity profiles based on in vitro bioassays revealed the occurrence of xenobiotic and steroid-like activities, including very high glucocorticoid, antimineralocorticoid, progestogenic and pregnane X receptor (PXR)-like activities (μg standard-EQ/g of sorbent range), and weak estrogenic activity (ng E2-EQ/g of sorbent range). Chemical analyses detected up to 60 out of 118 targeted steroid and pharmaceutical compounds in the extracts. In vitro profiling of occurring individual chemicals revealed the ability of several ones to act as agonist and/or antagonist of different steroids receptors. Mass balance calculation identified dexamethasone, spironolactone, and 6-alpha-methylprednisolone as major contributors to corticosteroid activities and levonorgestrel as the main contributor to progestogenic activities. Finally, RP-HPLC based fractionation of POCIS extracts and testing activity of fractions confirmed identified compounds and further revealed the presence of other unknown active chemicals. This study is one of the first to report environmental contamination by such chemicals; their possible contribution to in situ effects on fish at the same site is suggested.
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(23)
, 16726-16736. https://doi.org/10.1021/acs.est.2c02938
- Liying Yan, Qiuyu Rong, Hao Zhang, Kevin C. Jones, Yanying Li, Jun Luo. Evaluation and Application of a Novel Diffusive Gradients in Thin-Films Technique for In Situ Monitoring of Glucocorticoids in Natural Waters. Environmental Science & Technology 2022, 56
(22)
, 15499-15507. https://doi.org/10.1021/acs.est.2c00834
- Ahmed Tlili, Natàlia Corcoll, Åsa Arrhenius, Thomas Backhaus, Juliane Hollender, Nicolas Creusot, Bettina Wagner, Renata Behra. Tolerance Patterns in Stream Biofilms Link Complex Chemical Pollution to Ecological Impacts. Environmental Science & Technology 2020, 54
(17)
, 10745-10753. https://doi.org/10.1021/acs.est.0c02975
- Clémentine Garoche, Selim Aït-Aïssa, Abdelhay Boulahtouf, Nicolas Creusot, Nathalie Hinfray, William Bourguet, Patrick Balaguer, François Brion. Human and Zebrafish Nuclear Progesterone Receptors Are Differently Activated by Manifold Progestins. Environmental Science & Technology 2020, 54
(15)
, 9510-9518. https://doi.org/10.1021/acs.est.0c02056
- Peta A. Neale, Georg Braun, Werner Brack, Eric Carmona, Roman Gunold, Maria König, Martin Krauss, Liana Liebmann, Matthias Liess, Moritz Link, Ralf B. Schäfer, Rita Schlichting, Verena C. Schreiner, Tobias Schulze, Philipp Vormeier, Oliver Weisner, Beate I. Escher. Assessing the Mixture Effects in In Vitro Bioassays of Chemicals Occurring in Small Agricultural Streams during Rain Events. Environmental Science & Technology 2020, 54
(13)
, 8280-8290. https://doi.org/10.1021/acs.est.0c02235
- Alexander Weizel, Michael P. Schlüsener, Georg Dierkes, Thomas A. Ternes. Occurrence of Glucocorticoids, Mineralocorticoids, and Progestogens in Various Treated Wastewater, Rivers, and Streams. Environmental Science & Technology 2018, 52
(9)
, 5296-5307. https://doi.org/10.1021/acs.est.7b06147
- Juliane Hollender, Emma L. Schymanski, Heinz P. Singer, and P. Lee Ferguson . Nontarget Screening with High Resolution Mass Spectrometry in the Environment: Ready to Go?. Environmental Science & Technology 2017, 51
(20)
, 11505-11512. https://doi.org/10.1021/acs.est.7b02184
- Edward J. Perkins, Tanwir Habib, Barbara L. Escalon, Jenna E. Cavallin, Linnea Thomas, Matthew Weberg, Megan N. Hughes, Kathleen M. Jensen, Michael D. Kahl, Daniel L. Villeneuve, Gerald T. Ankley, and Natàlia Garcia-Reyero . Prioritization of Contaminants of Emerging Concern in Wastewater Treatment Plant Discharges Using Chemical:Gene Interactions in Caged Fish. Environmental Science & Technology 2017, 51
(15)
, 8701-8712. https://doi.org/10.1021/acs.est.7b01567
- Susan D. Richardson and Susana Y. Kimura . Water Analysis: Emerging Contaminants and Current Issues. Analytical Chemistry 2016, 88
(1)
, 546-582. https://doi.org/10.1021/acs.analchem.5b04493
- Vimal Kumar, Andrew C. Johnson, Achim Trubiroha, Jitka Tumová, Masaru Ihara, Roman Grabic, Werner Kloas, Hiroaki Tanaka, and Hana Kocour Kroupová . The Challenge Presented by Progestins in Ecotoxicological Research: A Critical Review. Environmental Science & Technology 2015, 49
(5)
, 2625-2638. https://doi.org/10.1021/es5051343
- Frederic D.L. Leusch, Hamish Allen, Nuwan A.L. De Silva, Roger Hodson, Matthew Johnson, Peta A. Neale, Michael Stewart, Louis A. Tremblay, Taylor Wilde, Grant L. Northcott. Effect-based monitoring of two rivers under urban and agricultural influence reveals a range of biological activities in sediment and water extracts. Journal of Environmental Management 2024, 351 , 119692. https://doi.org/10.1016/j.jenvman.2023.119692
- Qingyue Xiang, Xiaoyan Shen, Kun Li, Zihao Wang, Xinkun Zhao, Qingfeng Chen. Occurrence, distribution, and environmental risk of 61 glucocorticoids in surface water of the Yellow River Delta, China. Science of The Total Environment 2024, 906 , 167504. https://doi.org/10.1016/j.scitotenv.2023.167504
- Bismark Anabila Nyaaba, Joseph Nana Gyesi, Godfred Darko, Lawrence Sheringham Borquaye. Occurrence and Ecological Risk Assessment of Pharmaceutical Residues in Effluents from Pharmaceutical Manufacturing Facilities in Kumasi, Ghana. Chemistry Africa 2023, 6
(6)
, 3131-3143. https://doi.org/10.1007/s42250-023-00703-6
- Veronica Margarita Gutiérrez-Noya, Leobardo Manuel Gómez-Oliván, Idalia Casas-Hinojosa, Sandra García-Medina, Karina Elisa Rosales-Pérez, José Manuel Orozco-Hernández, Gustavo Axel Elizalde-Velázquez, Marcela Galar-Martínez, Octavio Dublán-García, Hariz Islas-Flores. Short-term exposure to dexamethasone at environmentally relevant concentrations impairs embryonic development in Cyprinus carpio: Bioconcentration and alteration of oxidative stress-related gene expression patterns. Science of The Total Environment 2023, 898 , 165528. https://doi.org/10.1016/j.scitotenv.2023.165528
- Jian Gong, Ke-Xin Yang, Can-Yuan Lin, Qiang Li, Chong Han, Wei Tao, Ying Huang, Wei-Quan Lin, Cui-Qin Wu, Shu-Han Zhang, De-Xin Wang. Prevalence, distribution, accumulation, and risk of environmental corticosteroids and estrogens in biofilms from the Pearl River Delta. Environmental Pollution 2023, 334 , 122192. https://doi.org/10.1016/j.envpol.2023.122192
- Pavel Šauer, Branislav Vrana, Beate I. Escher, Roman Grabic, Zuzana Toušová, Martin Krauss, Peter C. von der Ohe, Maria König, Kateřina Grabicová, Petra Mikušová, Roman Prokeš, Jaromír Sobotka, Pavla Fialová, Jiří Novák, Werner Brack, Klára Hilscherová. Bioanalytical and chemical characterization of organic micropollutant mixtures in long-term exposed passive samplers from the Joint Danube Survey 4: Setting a baseline for water quality monitoring. Environment International 2023, 178 , 107957. https://doi.org/10.1016/j.envint.2023.107957
- Jian Gong, Yong-Shun Zhou, Can-Yuan Lin, Qiang Li, Chong Han, Ke-Xin Yang, Ying Huang, Wei-Quan Lin, Cui-Qin Wu, Shu-Han Zhang, Jia-Yu Huang. Suspended particulate matter-associated environmental corticosteroids in the Pearl River, China: Occurrence, distribution, and partitioning. Science of The Total Environment 2023, 884 , 163701. https://doi.org/10.1016/j.scitotenv.2023.163701
- Akiho Watanabe, Taijun Myosho, Ayaka Ishibashi, Jun Yamamoto, Misa Toda, Yuta Onishi, Tohru Kobayashi. Levonorgestrel causes feminization and dose-dependent masculinization in medaka fish (Oryzias latipes): Endocrine-disruption activity and its correlation with sex reversal. Science of The Total Environment 2023, 876 , 162740. https://doi.org/10.1016/j.scitotenv.2023.162740
- Clémentine Garoche, Marina Grimaldi, Erwan Michelin, Abdelhay Boulahtouf, Anthony Marconi, François Brion, Patrick Balaguer, Selim Aït-Aïssa. Interlaboratory prevalidation of a new in vitro transcriptional activation assay for the screening of (anti-)androgenic activity of chemicals using the UALH-hAR cell line. Toxicology in Vitro 2023, 88 , 105554. https://doi.org/10.1016/j.tiv.2023.105554
- Peta A. Neale, Beate I. Escher, Milo L. de Baat, Jérôme Enault, Frederic D. L. Leusch. Effect‐Based Trigger Values Are Essential for the Uptake of Effect‐Based Methods in Water Safety Planning. Environmental Toxicology and Chemistry 2023, 42
(3)
, 714-726. https://doi.org/10.1002/etc.5544
- Saskia Finckh, Sebastian Buchinger, Beate I. Escher, Henner Hollert, Maria König, Martin Krauss, Warich Leekitratanapisan, Sabrina Schiwy, Rita Schlichting, Aliaksandra Shuliakevich, Werner Brack. Endocrine disrupting chemicals entering European rivers: Occurrence and adverse mixture effects in treated wastewater. Environment International 2022, 170 , 107608. https://doi.org/10.1016/j.envint.2022.107608
- Magda Caban, Hanna Lis, Piotr Stepnowski. Limitations of Integrative Passive Samplers as a Tool for the Quantification of Pharmaceuticals in the Environment – A Critical Review with the Latest Innovations. Critical Reviews in Analytical Chemistry 2022, 52
(6)
, 1386-1407. https://doi.org/10.1080/10408347.2021.1881755
- Miki Jahn, Frank Seebacher. Variations in cost of transport and their ecological consequences: a review. Journal of Experimental Biology 2022, 225
(15)
https://doi.org/10.1242/jeb.243646
- Wen-Jun Shi, Xiao-Bing Long, Si-Ying Li, Dong-Dong Ma, Fang Liu, Jin-Ge Zhang, Zhi-Jie Lu, Guang-Guo Ying. Dydrogesterone and levonorgestrel at environmentally relevant concentrations have antagonist effects with rhythmic oscillation in brain and eyes of zebrafish. Aquatic Toxicology 2022, 248 , 106177. https://doi.org/10.1016/j.aquatox.2022.106177
- Ai Zhang, Yongqiang Ding, Ai Jia, Minkyu Park, Kevin D. Daniels, Xuhao Nie, Shimin Wu, Shane A. Snyder. Removal of 26 corticosteroids, potential COVID-19 remedies, at environmentally relevant concentrations in water using UV/free chlorine, UV/monochloramine, and UV/hydrogen peroxide. Environmental Science: Water Research & Technology 2022, 8
(5)
, 1078-1091. https://doi.org/10.1039/D1EW00919B
- Hannah J. Petty, Jacob E. Barrett, Erin G. Kosmowski, Dandre S. Amos, Sean M. Ryan, Lucas D. Jones, Christopher S. Lassiter. Spironolactone affects cardiovascular and craniofacial development in zebrafish embryos (Danio rerio). Environmental Toxicology and Pharmacology 2022, 92 , 103848. https://doi.org/10.1016/j.etap.2022.103848
- Julie Robitaille, Nancy D. Denslow, Beate I. Escher, Hajime G. Kurita-Oyamada, Vicki Marlatt, Christopher J. Martyniuk, Laia Navarro-Martín, Ryan Prosser, Thomas Sanderson, Viviane Yargeau, Valerie S. Langlois. Towards regulation of Endocrine Disrupting chemicals (EDCs) in water resources using bioassays – A guide to developing a testing strategy. Environmental Research 2022, 205 , 112483. https://doi.org/10.1016/j.envres.2021.112483
- Xiaoting Gao, Keliang Huang, Ai Zhang, Cihao Wang, Zhuyu Sun, Yanan Liu. Simultaneous degradation of glucocorticoids and sterilization using bubbling corona discharge plasma based systems: A promising terminal water treatment facility for hospital wastewater. Chemical Engineering Journal 2022, 430 , 132845. https://doi.org/10.1016/j.cej.2021.132845
- Katia Pes, Juan B. Ortiz-Delgado, Carmen Sarasquete, Vincent Laizé, Ignacio Fernández. Short-term exposure to pharmaceuticals negatively impacts marine flatfish species: Histological, biochemical and molecular clues for an integrated ecosystem risk assessment. Environmental Toxicology and Pharmacology 2022, 90 , 103822. https://doi.org/10.1016/j.etap.2022.103822
- Darryl W. Hawker, Joseph Clokey, Sara G. Gorji, Rory Verhagen, Sarit L. Kaserzon. Monitoring techniques–Grab and passive sampling. 2022, 25-48. https://doi.org/10.1016/B978-0-12-822850-0.00014-4
- Anita A. Thambirajah, Michael G. Wade, Jonathan Verreault, Nicolas Buisine, Verônica A. Alves, Valerie S. Langlois, Caren C. Helbing. Disruption by stealth - Interference of endocrine disrupting chemicals on hormonal crosstalk with thyroid axis function in humans and other animals. Environmental Research 2022, 203 , 111906. https://doi.org/10.1016/j.envres.2021.111906
- Azeez Yusuf, Dylan O'Flynn, Blanaid White, Linda Holland, Anne Parle-McDermott, Jenny Lawler, Thomas McCloughlin, Denise Harold, Belinda Huerta, Fiona Regan. Monitoring of emerging contaminants of concern in the aquatic environment: a review of studies showing the application of effect-based measures. Analytical Methods 2021, 13
(43)
, 5120-5143. https://doi.org/10.1039/D1AY01184G
- Wen-Jun Shi, Dong-Dong Ma, Gui-Zhen Fang, Jin-Ge Zhang, Guo-Yong Huang, Lingtian Xie, Hong-Xing Chen, Li-Ping Hou, Guang-Guo Ying. Levonorgestrel and dydrogesterone affect sex determination via different pathways in zebrafish. Aquatic Toxicology 2021, 240 , 105972. https://doi.org/10.1016/j.aquatox.2021.105972
- J.O. Ojoghoro, M.D. Scrimshaw, J.P. Sumpter. Steroid hormones in the aquatic environment. Science of The Total Environment 2021, 792 , 148306. https://doi.org/10.1016/j.scitotenv.2021.148306
- Li Zhao, Hong Chang, Fuhong Sun, Hailei Su. Determination and Occurrence of Mineralocorticoids in Taihu Lake of China. Frontiers in Environmental Science 2021, 9 https://doi.org/10.3389/fenvs.2021.727645
- Jihyun Cha, Seongjin Hong, Junghyun Lee, Jiyun Gwak, Mungi Kim, Taewoo Kim, Jin Hur, John P. Giesy, Jong Seong Khim. Novel polar AhR-active chemicals detected in sediments of an industrial area using effect-directed analysis based on in vitro bioassays with full-scan high resolution mass spectrometric screening. Science of The Total Environment 2021, 779 , 146566. https://doi.org/10.1016/j.scitotenv.2021.146566
- Katia Pes, Annika Friese, Cymon J. Cox, Vincent Laizé, Ignacio Fernández. Biochemical and molecular responses of the Mediterranean mussel (Mytilus galloprovincialis) to short-term exposure to three commonly prescribed drugs. Marine Environmental Research 2021, 168 , 105309. https://doi.org/10.1016/j.marenvres.2021.105309
- Reza Lotfi Khatoonabadi, Maryam Vosough, Lotta L. Hohrenk, Torsten C. Schmidt. Employing complementary multivariate methods for a designed nontarget LC-HRMS screening of a wastewater-influenced river. Microchemical Journal 2021, 160 , 105641. https://doi.org/10.1016/j.microc.2020.105641
- Shazia Farzana, Yuefei Ruan, Qi Wang, Rongben Wu, Zhang Kai, Yan Meng, Kenneth M.Y. Leung, Paul K.S. Lam. Developing interim water quality criteria for emerging chemicals of concern for protecting marine life in the Greater Bay Area of South China. Marine Pollution Bulletin 2020, 161 , 111792. https://doi.org/10.1016/j.marpolbul.2020.111792
- Muhammad Bilal, S. Salman Ashraf, Luiz Fernando Romanholo Ferreira, Jiandong Cui, Wen-Yong Lou, Marcelo Franco, Hafiz M.N. Iqbal. Nanostructured materials as a host matrix to develop robust peroxidases-based nanobiocatalytic systems. International Journal of Biological Macromolecules 2020, 162 , 1906-1923. https://doi.org/10.1016/j.ijbiomac.2020.08.122
- Peta A. Neale, Marina Grimaldi, Abdelhay Boulahtouf, Frederic D.L. Leusch, Patrick Balaguer. Assessing species-specific differences for nuclear receptor activation for environmental water extracts. Water Research 2020, 185 , 116247. https://doi.org/10.1016/j.watres.2020.116247
- Anthony Gravell, Gary R. Fones, Richard Greenwood, Graham A. Mills. Detection of pharmaceuticals in wastewater effluents—a comparison of the performance of Chemcatcher® and polar organic compound integrative sampler. Environmental Science and Pollution Research 2020, 27
(22)
, 27995-28005. https://doi.org/10.1007/s11356-020-09077-5
- Hélène Serra, François Brion, Clémence Chardon, Hélène Budzinski, Tobias Schulze, Werner Brack, Selim Aït-Aïssa. Estrogenic activity of surface waters using zebrafish- and human-based in vitro assays: The Danube as a case-study. Environmental Toxicology and Pharmacology 2020, 78 , 103401. https://doi.org/10.1016/j.etap.2020.103401
- Lucia Toporova, Patrick Balaguer. Nuclear receptors are the major targets of endocrine disrupting chemicals. Molecular and Cellular Endocrinology 2020, 502 , 110665. https://doi.org/10.1016/j.mce.2019.110665
- Klaudia Godlewska, Piotr Stepnowski, Monika Paszkiewicz. Application of the Polar Organic Chemical Integrative Sampler for Isolation of Environmental Micropollutants – A Review. Critical Reviews in Analytical Chemistry 2020, 50
(1)
, 1-28. https://doi.org/10.1080/10408347.2019.1565983
- Muhammad Arslan Kamal Hashmi, Martin Krauss, Beate I. Escher, Ivana Teodorovic, Werner Brack. Effect‐Directed Analysis of Progestogens and Glucocorticoids at Trace Concentrations in River Water. Environmental Toxicology and Chemistry 2020, 39
(1)
, 189-199. https://doi.org/10.1002/etc.4609
- Ana Lourdes Oropesa, Laura Guimarães. Occurrence of Levonorgestrel in Water Systems and Its Effects on Aquatic Organisms: A Review. 2020, 57-84. https://doi.org/10.1007/398_2020_44
- Peta A. Neale, Beate I. Escher. Mixture Modelling and Effect-Directed Analysis for Identification of Chemicals, Mixtures and Effects of Concern. 2020, 87-97. https://doi.org/10.1007/978-981-13-9447-8_7
- Pavel Šauer, Jitka Tumová, Christoph Steinbach, Oksana Golovko, Hans Komen, Emmanuelle Maillot-Maréchal, Jana Máchová, Roman Grabic, Selim Aït-Aïssa, Hana Kocour Kroupová. Chronic simultaneous exposure of common carp (Cyprinus carpio) from embryonic to juvenile stage to drospirenone and gestodene at low ng/L level caused intersex. Ecotoxicology and Environmental Safety 2020, 188 , 109912. https://doi.org/10.1016/j.ecoenv.2019.109912
- Yanan Liu, Cihao Wang, Xue Shen, Ai Zhang, Shuwen Yan, Xiang Li, Andere Clement Miruka, Shimin Wu, Ying Guo, Stéphanie Ognier. Degradation of glucocorticoids in aqueous solution by dielectric barrier discharge: Kinetics, mechanisms, and degradation pathways. Chemical Engineering Journal 2019, 374 , 412-428. https://doi.org/10.1016/j.cej.2019.05.154
- Jian Gong, Canyuan Lin, Xiaoping Xiong, Diyun Chen, Yongheng Chen, Yongshun Zhou, Cuiqin Wu, Yongming Du. Occurrence, distribution, and potential risks of environmental corticosteroids in surface waters from the Pearl River Delta, South China. Environmental Pollution 2019, 251 , 102-109. https://doi.org/10.1016/j.envpol.2019.04.110
- Eszter Simon, Andrea Schifferli, Thomas B. Bucher, Daniel Olbrich, Inge Werner, Etiënne L. M. Vermeirssen. Solid-phase extraction of estrogens and herbicides from environmental waters for bioassay analysis—effects of sample volume on recoveries. Analytical and Bioanalytical Chemistry 2019, 411
(10)
, 2057-2069. https://doi.org/10.1007/s00216-019-01628-1
- Jéssica C. Zanette, Márcia T. Veit, Gilberto C. Gonçalves, Soraya M. Palácio, Fernando R. Scremin, Alex S. Torquato, Márcia R. S. A. Vieira. A study on the removal of prednisone from aqueous solutions by adsorption onto a vegetal activated carbon. Water Science and Technology 2018, 78
(11)
, 2328-2337. https://doi.org/10.2166/wst.2018.515
- Pavel Šauer, Adam Bořík, Oksana Golovko, Roman Grabic, Andrea Vojs Staňová, Olga Valentová, Alžběta Stará, Marie Šandová, Hana Kocour Kroupová. Do progestins contribute to (anti-)androgenic activities in aquatic environments?. Environmental Pollution 2018, 242 , 417-425. https://doi.org/10.1016/j.envpol.2018.06.104
- Yoonah Jeong, Andreas Schäffer, Kilian Smith. A comparison of equilibrium and kinetic passive sampling for the monitoring of aquatic organic contaminants in German rivers. Water Research 2018, 145 , 248-258. https://doi.org/10.1016/j.watres.2018.08.016
- Guohong Liu, Zhaohan Zhang, Chen Yan, Yang Wang, Xuerui Ma, Peng Gao, Yujie Feng. Adsorption of estrone with few-layered boron nitride nanosheets: Kinetics, thermodynamics and mechanism. Chemosphere 2018, 207 , 534-542. https://doi.org/10.1016/j.chemosphere.2018.05.129
- Tia-Marie Scott, Patrick J. Phillips, Dana W. Kolpin, Kaitlyn M. Colella, Edward T. Furlong, William T. Foreman, James L. Gray. Pharmaceutical manufacturing facility discharges can substantially increase the pharmaceutical load to U.S. wastewaters. Science of The Total Environment 2018, 636 , 69-79. https://doi.org/10.1016/j.scitotenv.2018.04.160
- Manoj Sonavane, Jennifer E. Schollée, Anita O. Hidasi, Nicolas Creusot, François Brion, Marc J.‐F. Suter, Juliane Hollender, Selim Aїt‐Aїssa. An integrative approach combining passive sampling, bioassays, and effect‐directed analysis to assess the impact of wastewater effluent. Environmental Toxicology and Chemistry 2018, 37
(8)
, 2079-2088. https://doi.org/10.1002/etc.4155
- Ai Zhang, Xue Shen, Xueyan Yin, Xiang Li, Yanan Liu. Application of calcium peroxide for efficient removal of triamcinolone acetonide from aqueous solutions: Mechanisms and products. Chemical Engineering Journal 2018, 345 , 594-603. https://doi.org/10.1016/j.cej.2018.01.104
- Guiying Li, Huan Yang, Taicheng An, Yujuan Lu. Antibiotics elimination and risk reduction at two drinking water treatment plants by using different conventional treatment techniques. Ecotoxicology and Environmental Safety 2018, 158 , 154-161. https://doi.org/10.1016/j.ecoenv.2018.04.019
- Victoria Osorio, Merijn Schriks, Dennis Vughs, Pim de Voogt, Annemieke Kolkman. A novel sample preparation procedure for effect-directed analysis of micro-contaminants of emerging concern in surface waters. Talanta 2018, 186 , 527-537. https://doi.org/10.1016/j.talanta.2018.04.058
- Frederic D.L. Leusch, Peta A. Neale, Charlotte Arnal, Natalie H. Aneck-Hahn, Patrick Balaguer, Auguste Bruchet, Beate I. Escher, Mar Esperanza, Marina Grimaldi, Gaela Leroy, Marco Scheurer, Rita Schlichting, Merijn Schriks, Armelle Hebert. Analysis of endocrine activity in drinking water, surface water and treated wastewater from six countries. Water Research 2018, 139 , 10-18. https://doi.org/10.1016/j.watres.2018.03.056
- Huizhen Li, Jie Zhang, Jing You. Diagnosis of complex mixture toxicity in sediments: Application of toxicity identification evaluation (TIE) and effect-directed analysis (EDA). Environmental Pollution 2018, 237 , 944-954. https://doi.org/10.1016/j.envpol.2017.11.005
- Pavel Šauer, Alžběta Stará, Oksana Golovko, Olga Valentová, Adam Bořík, Roman Grabic, Hana Kocour Kroupová. Two synthetic progestins and natural progesterone are responsible for most of the progestagenic activities in municipal wastewater treatment plant effluents in the Czech and Slovak republics. Water Research 2018, 137 , 64-71. https://doi.org/10.1016/j.watres.2018.02.065
- Tara J. Thrupp, Tamsin J. Runnalls, Martin Scholze, Subramaniam Kugathas, Andreas Kortenkamp, John P. Sumpter. The consequences of exposure to mixtures of chemicals: Something from ‘nothing’ and ‘a lot from a little’ when fish are exposed to steroid hormones. Science of The Total Environment 2018, 619-620 , 1482-1492. https://doi.org/10.1016/j.scitotenv.2017.11.081
- Diego O. Murillo-Martínez, Daniel E. León-Perez, Gloria J. Correa-Restrepo, Julián Londoño-Londoño, Claudio Jiménez-Cartagena. Passive Sampling to Monitor Hazardous Compounds in Water: A Tool for the Risk Assessment of Consuming Aquatic Food. 2018, 373-406. https://doi.org/10.1016/B978-0-12-811445-2.00011-8
- Chimi Wangmo, Sergio Jarque, Klára Hilscherová, Luděk Bláha, Michal Bittner. In vitro
assessment of sex steroids and related compounds in water and sediments – a critical review. Environmental Science: Processes & Impacts 2018, 20
(2)
, 270-287. https://doi.org/10.1039/C7EM00458C
- Peta A. Neale, Werner Brack, Selim Aït-Aïssa, Wibke Busch, Juliane Hollender, Martin Krauss, Emmanuelle Maillot-Maréchal, Nicole A. Munz, Rita Schlichting, Tobias Schulze, Bernadette Vogler, Beate I. Escher. Solid-phase extraction as sample preparation of water samples for cell-based and other
in vitro
bioassays. Environmental Science: Processes & Impacts 2018, 20
(3)
, 493-504. https://doi.org/10.1039/C7EM00555E
- Fabian Itzel, Linda Gehrmann, Helena Bielak, Paul Ebersbach, Andrea Boergers, Heinrich Herbst, Christian Maus, Anne Simon, Elke Dopp, Monika Hammers-Wirtz, Torsten C Schmidt, Jochen Tuerk. Investigation of full-scale ozonation at a municipal wastewater treatment plant using a toxicity-based evaluation concept. Journal of Toxicology and Environmental Health, Part A 2017, 80
(23-24)
, 1242-1258. https://doi.org/10.1080/15287394.2017.1369663
- Thomas W.K. Fraser, Abdolrahman Khezri, Anna M. Lewandowska-Sabat, Theodore Henry, Erik Ropstad. Endocrine disruptors affect larval zebrafish behavior: Testing potential mechanisms and comparisons of behavioral sensitivity to alternative biomarkers. Aquatic Toxicology 2017, 193 , 128-135. https://doi.org/10.1016/j.aquatox.2017.10.002
- Zuzana Tousova, Peter Oswald, Jaroslav Slobodnik, Ludek Blaha, Melis Muz, Meng Hu, Werner Brack, Martin Krauss, Carolina Di Paolo, Zsolt Tarcai, Thomas-Benjamin Seiler, Henner Hollert, Sanja Koprivica, Marijan Ahel, Jennifer E. Schollée, Juliane Hollender, Marc J.-F. Suter, Anita O. Hidasi, Kristin Schirmer, Manoj Sonavane, Selim Ait-Aissa, Nicolas Creusot, Francois Brion, Jean Froment, Ana Catarina Almeida, Kevin Thomas, Knut Erik Tollefsen, Sara Tufi, Xiyu Ouyang, Pim Leonards, Marja Lamoree, Victoria Osorio Torrens, Annemieke Kolkman, Merijn Schriks, Petra Spirhanzlova, Andrew Tindall, Tobias Schulze. European demonstration program on the effect-based and chemical identification and monitoring of organic pollutants in European surface waters. Science of The Total Environment 2017, 601-602 , 1849-1868. https://doi.org/10.1016/j.scitotenv.2017.06.032
- Navdeep Bal, Anu Kumar, Dayanthi Nugegoda. Assessing multigenerational effects of prednisolone to the freshwater snail, Physa acuta (Gastropoda: Physidae). Journal of Hazardous Materials 2017, 339 , 281-291. https://doi.org/10.1016/j.jhazmat.2017.06.024
- Gracia Rivas Ibáñez, Michal Bittner, Zuzana Toušová, Marina Celia Campos‐Mañas, Ana Agüera, José Luis Casas López, José Antonio Sánchez Pérez, Klára Hilscherová. Does micropollutant removal by solar photo‐Fenton reduce ecotoxicity in municipal wastewater? A comprehensive study at pilot scale open reactors. Journal of Chemical Technology & Biotechnology 2017, 92
(8)
, 2114-2122. https://doi.org/10.1002/jctb.5212
- Andrea Ziková, Claudia Lorenz, Frauke Hoffmann, Wibke Kleiner, Ilka Lutz, Matthias Stöck, Werner Kloas. Endocrine disruption by environmental gestagens in amphibians – A short review supported by new in vitro data using gonads of Xenopus laevis. Chemosphere 2017, 181 , 74-82. https://doi.org/10.1016/j.chemosphere.2017.04.021
- Cheryl S. Rosenfeld, Nancy D. Denslow, Edward F. Orlando, Juan Manuel Gutierrez-Villagomez, Vance L Trudeau. Neuroendocrine disruption of organizational and activational hormone programming in poikilothermic vertebrates. Journal of Toxicology and Environmental Health, Part B 2017, 20
(5)
, 276-304. https://doi.org/10.1080/10937404.2017.1370083
- Navdeep Bal, Anupama Kumar, Jun Du, Dayanthi Nugegoda. Multigenerational effects of two glucocorticoids (prednisolone and dexamethasone) on life-history parameters of crustacean Ceriodaphnia dubia (Cladocera). Environmental Pollution 2017, 225 , 569-578. https://doi.org/10.1016/j.envpol.2017.03.024
- Qiyu Chen, Caixia Li, Zhiyuan Gong, Eric Chun Yong Chan, Shane A. Snyder, Siew Hong Lam. Common deregulated gene expression profiles and morphological changes in developing zebrafish larvae exposed to environmental-relevant high to low concentrations of glucocorticoids. Chemosphere 2017, 172 , 429-439. https://doi.org/10.1016/j.chemosphere.2017.01.036
- Anita O. Hidasi, Ksenia J. Groh, Marc J.-F. Suter, Kristin Schirmer. Clobetasol propionate causes immunosuppression in zebrafish (Danio rerio) at environmentally relevant concentrations. Ecotoxicology and Environmental Safety 2017, 138 , 16-24. https://doi.org/10.1016/j.ecoenv.2016.11.024
- J.M. Davis, D.R. Ekman, D.M. Skelton, C.A. LaLone, G.T. Ankley, J.E. Cavallin, D.L. Villeneuve, T.W. Collette. Metabolomics for informing adverse outcome pathways: Androgen receptor activation and the pharmaceutical spironolactone. Aquatic Toxicology 2017, 184 , 103-115. https://doi.org/10.1016/j.aquatox.2017.01.001
- Ahmed Tlili, Juliane Hollender, Cornelia Kienle, Renata Behra. Micropollutant-induced tolerance of in situ periphyton: Establishing causality in wastewater-impacted streams. Water Research 2017, 111 , 185-194. https://doi.org/10.1016/j.watres.2017.01.016
- Peta A. Neale, Nicole A. Munz, Selim Aїt-Aїssa, Rolf Altenburger, François Brion, Wibke Busch, Beate I. Escher, Klára Hilscherová, Cornelia Kienle, Jiří Novák, Thomas-Benjamin Seiler, Ying Shao, Christian Stamm, Juliane Hollender. Integrating chemical analysis and bioanalysis to evaluate the contribution of wastewater effluent on the micropollutant burden in small streams. Science of The Total Environment 2017, 576 , 785-795. https://doi.org/10.1016/j.scitotenv.2016.10.141
- Jing You, Huizhen Li. Improving the accuracy of effect-directed analysis: the role of bioavailability. Environmental Science: Processes & Impacts 2017, 19
(12)
, 1484-1498. https://doi.org/10.1039/C7EM00377C
- N. Hinfray, C. Tebby, C. Garoche, B. Piccini, G. Bourgine, S. Aït-Aïssa, O. Kah, F. Pakdel, F. Brion. Additive effects of levonorgestrel and ethinylestradiol on brain aromatase ( cyp19a1b ) in zebrafish specific in vitro and in vivo bioassays. Toxicology and Applied Pharmacology 2016, 307 , 108-114. https://doi.org/10.1016/j.taap.2016.07.023
- Joel Cano-Nicolau, Clémentine Garoche, Nathalie Hinfray, Elisabeth Pellegrini, Noureddine Boujrad, Farzad Pakdel, Olivier Kah, François Brion. Several synthetic progestins disrupt the glial cell specific-brain aromatase expression in developing zebra fish. Toxicology and Applied Pharmacology 2016, 305 , 12-21. https://doi.org/10.1016/j.taap.2016.05.019
- Nicolas Creusot, Marie-Hélène Dévier, Hélène Budzinski, Selim Aït-Aïssa. Evaluation of an extraction method for a mixture of endocrine disrupters in sediment using chemical and in vitro biological analyses. Environmental Science and Pollution Research 2016, 23
(11)
, 10349-10360. https://doi.org/10.1007/s11356-016-6062-1
- Nadine Madeleine Rossier, Geraldine Chew, Kun Zhang, Francesco Riva, Karl Fent. Activity of binary mixtures of drospirenone with progesterone and 17α-ethinylestradiol in vitro and in vivo. Aquatic Toxicology 2016, 174 , 109-122. https://doi.org/10.1016/j.aquatox.2016.02.005
- Manoj Sonavane, Nicolas Creusot, Emmanuelle Maillot-Maréchal, Alexandre Péry, François Brion, Selim Aїt-Aïssa. Zebrafish-based reporter gene assays reveal different estrogenic activities in river waters compared to a conventional human-derived assay. Science of The Total Environment 2016, 550 , 934-939. https://doi.org/10.1016/j.scitotenv.2016.01.187
- Adriana Wigh, Alain Devaux, Vanessa Brosselin, Adriana Gonzalez-Ospina, Bruno Domenjoud, Selim Aït-Aïssa, Nicolas Creusot, Antoine Gosset, Christine Bazin, Sylvie Bony. Proposal to optimize ecotoxicological evaluation of wastewater treated by conventional biological and ozonation processes. Environmental Science and Pollution Research 2016, 23
(4)
, 3008-3017. https://doi.org/10.1007/s11356-015-5419-1
- Qiyu Chen, Ai Jia, Shane A. Snyder, Zhiyuan Gong, Siew Hong Lam. Glucocorticoid activity detected by in vivo zebrafish assay and in vitro glucocorticoid receptor bioassay at environmental relevant concentrations. Chemosphere 2016, 144 , 1162-1169. https://doi.org/10.1016/j.chemosphere.2015.09.089
- Werner Brack, Selim Ait-Aissa, Robert M. Burgess, Wibke Busch, Nicolas Creusot, Carolina Di Paolo, Beate I. Escher, L. Mark Hewitt, Klara Hilscherova, Juliane Hollender, Henner Hollert, Willem Jonker, Jeroen Kool, Marja Lamoree, Matthias Muschket, Steffen Neumann, Pawel Rostkowski, Christoph Ruttkies, Jennifer Schollee, Emma L. Schymanski, Tobias Schulze, Thomas-Benjamin Seiler, Andrew J. Tindall, Gisela De Aragão Umbuzeiro, Branislav Vrana, Martin Krauss. Effect-directed analysis supporting monitoring of aquatic environments — An in-depth overview. Science of The Total Environment 2016, 544 , 1073-1118. https://doi.org/10.1016/j.scitotenv.2015.11.102
- Annika Jahnke, Gesine Witt, Sabine Schäfer, Nora Haase, Beate I. Escher. Combining Passive Sampling with Toxicological Characterization of Complex Mixtures of Pollutants from the Aquatic Environment. 2016, 225-261. https://doi.org/10.1007/10_2015_5014
- V. Arya, Ligy Philip, S. Murty Bhallamudi. Performance of suspended and attached growth bioreactors for the removal of cationic and anionic pharmaceuticals. Chemical Engineering Journal 2016, 284 , 1295-1307. https://doi.org/10.1016/j.cej.2015.09.070
- Ran Xu, Jianmei Yuan, Yifang Si, Fengting Li, Bingru Zhang. Estrone removal by horseradish peroxidase immobilized on a nanofibrous support with Fe
3
O
4
nanoparticles. RSC Advances 2016, 6
(5)
, 3927-3933. https://doi.org/10.1039/C5RA22805K
- Barry P. Baldigo, Scott D. George, Patrick J. Phillips, Jocelyn D.C. Hemming, Nancy D. Denslow, Kevin J. Kroll. Potential estrogenic effects of wastewaters on gene expression in
Pimephales promelas
and fish assemblages in streams of southeastern New York. Environmental Toxicology and Chemistry 2015, 34
(12)
, 2803-2815. https://doi.org/10.1002/etc.3120
- Graeme Allinson, Fujio Shiraishi, Ryo Kamata, Mayumi Allinson. Combining Passive Sampling with Recombinant Receptor–Reporter Gene Bioassays to Assess the Receptor Activity of Victorian Rivers. Bulletin of Environmental Contamination and Toxicology 2015, 95
(6)
, 758-763. https://doi.org/10.1007/s00128-015-1577-6
- Tamsin J. Runnalls, Nicola Beresford, Subramaniam Kugathas, Luigi Margiotta-Casaluci, Martin Scholze, Alexander P. Scott, John P. Sumpter. From single chemicals to mixtures—Reproductive effects of levonorgestrel and ethinylestradiol on the fathead minnow. Aquatic Toxicology 2015, 169 , 152-167. https://doi.org/10.1016/j.aquatox.2015.10.009
- Luigi Viganò, Silvio De Flora, Marco Gobbi, Giovanna Guiso, Alberto Izzotti, Alberta Mandich, Giuseppe Mascolo, Claudio Roscioli. Exposing native cyprinid (Barbus plebejus) juveniles to river sediments leads to gonadal alterations, genotoxic effects and thyroid disruption. Aquatic Toxicology 2015, 169 , 223-239. https://doi.org/10.1016/j.aquatox.2015.10.022
- Viktoriia Burkina, Sidika Sakalli, Martin Krøyer Rasmussen, Galia Zamaratskaia, Olga Koba, Giang Pham Thai, Roman Grabic, Tomas Randak, Vladimir Zlabek. Does dexamethasone affect hepatic CYP450 system of fish? Semi-static in-vivo experiment on juvenile rainbow trout. Chemosphere 2015, 139 , 155-162. https://doi.org/10.1016/j.chemosphere.2015.06.031
- Karl Fent. Progestins as endocrine disrupters in aquatic ecosystems: Concentrations, effects and risk assessment. Environment International 2015, 84 , 115-130. https://doi.org/10.1016/j.envint.2015.06.012
- Jenna Roberts, Peter A. Bain, Anupama Kumar, Christopher Hepplewhite, David J. Ellis, Andrew G. Christy, Sara G. Beavis. Tracking multiple modes of endocrine activity in Australia's largest inland sewage treatment plant and effluent‐ receiving environment using a panel of in vitro bioassays. Environmental Toxicology and Chemistry 2015, 34
(10)
, 2271-2281. https://doi.org/10.1002/etc.3051