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Fluorescence Characterization of IHSS Humic Substances:  Total Luminescence Spectra with Absorbance Correction

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Department of Chemistry, Gross Chemical Laboratory, Box 90346, Duke University, Durham, North Carolina 27708
Cite this: Environ. Sci. Technol. 1996, 30, 10, 3061–3065
Publication Date (Web):September 26, 1996
https://doi.org/10.1021/es960132l
Copyright © 1996 American Chemical Society
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Abstract

Total luminescence spectroscopy was applied to the fluorescence characterization of humic substances obtained from the International Humic Substances Society (IHSS). Results show that total luminescence spectra, represented as excitation−emission matrices (EEMs), may be used to discriminate between soil-derived and aquatic-derived IHSS humic substances and between humic and fulvic acids derived from the same source (soil or aquatic). Ionic strength in the range of 0−1 M KCl and humic substance concentration in the range of 5−100 mg/L had little effect on the fluorescence spectral characteristics of the humic substances, while pH had significant effects as expected. Absorbance correction was shown to be essential for accurate representation and comparison of the EEMs of the humic substances at high concentrations.

†

 Present address:  Chemistry Department, Ohio Wesleyan University, Delaware, OH 43015.

*

 Corresponding author telephone:  (919) 660-1545; fax:  (919) 660-1605; e-mail address:  [email protected]

✗

 Abstract published in Advance ACS Abstracts, August 1, 1996.

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  214. Robert G.M. Spencer, Lucy Bolton, Andy Baker. Freeze/thaw and pH effects on freshwater dissolved organic matter fluorescence and absorbance properties from a number of UK locations. Water Research 2007, 41 (13) , 2941-2950. https://doi.org/10.1016/j.watres.2007.04.012
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  246. Andy Baker, Roger Inverarity. Protein-like fluorescence intensity as a possible tool for determining river water quality. Hydrological Processes 2004, 18 (15) , 2927-2945. https://doi.org/10.1002/hyp.5597
  247. Fu Pingqing, Wu Fengchang, Liu Congqiang. Fluorescence excitation-emission matrix characterization of a commercial humic acid. Chinese Journal of Geochemistry 2004, 23 (4) , 309-318. https://doi.org/10.1007/BF02871302
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  259. Andy Baker, Roger Inverarity, Martin Charlton, Susie Richmond. Detecting river pollution using fluorescence spectrophotometry: case studies from the Ouseburn, NE England. Environmental Pollution 2003, 124 (1) , 57-70. https://doi.org/10.1016/S0269-7491(02)00408-6
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  266. Fritz H Frimmel, Marcus Assenmacher, Michael U Kumke, Christian Specht, Gudrun Abbt-Braun, Gudrun Gräbe. Removal of hydrophilic compounds from water with organic polymers. Chemical Engineering and Processing: Process Intensification 2002, 41 (9) , 731-736. https://doi.org/10.1016/S0255-2701(01)00193-3
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