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In Vitro Reconstitution of Eukaryotic Ion Channels Using Droplet Interface Bilayers

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    In Vitro Reconstitution of Eukaryotic Ion Channels Using Droplet Interface Bilayers
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    † Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford OX1 3TA, United Kingdom
    ‡ Department of Physiology, Anatomy and Genetics, Le Gros Clark Building, South Parks Road, University of Oxford, Oxford OX1 3QX, United Kingdom
    § Department of Physics, University of Oxford, Parks Road, Oxford OX1 3PU, United Kingdom
    [email protected]
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    Journal of the American Chemical Society

    Cite this: J. Am. Chem. Soc. 2011, 133, 24, 9370–9375
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    https://doi.org/10.1021/ja200128n
    Published May 18, 2011
    Copyright © 2011 American Chemical Society
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    Abstract

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    The ability to routinely study eukaryotic ion channels in a synthetic lipid environment would have a major impact on our understanding of how different lipids influence ion channel function. Here, we describe a straightforward, detergent-free method for the in vitro reconstitution of eukaryotic ion channels and ionotropic receptors into droplet interface bilayers and measure their electrical activity at both the macroscopic and single-channel level. We explore the general applicability of this method by reconstitution of channels from a wide range of sources including recombinant cell lines and native tissues, as well as preparations that are difficult to study by conventional methods including erythrocytes and mitochondria.

    Copyright © 2011 American Chemical Society

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    Additional description of the reconstitution method, and further electrical characterization of ion channels present in this manuscript are included as Supporting Information. This information is available free of charge via the Internet at http://pubs.acs.org/.

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    Cite this: J. Am. Chem. Soc. 2011, 133, 24, 9370–9375
    Click to copy citationCitation copied!
    https://doi.org/10.1021/ja200128n
    Published May 18, 2011
    Copyright © 2011 American Chemical Society
    Request reuse permissions

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