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In Vitro Reconstitution of Eukaryotic Ion Channels Using Droplet Interface Bilayers

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† Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford OX1 3TA, United Kingdom
‡ Department of Physiology, Anatomy and Genetics, Le Gros Clark Building, South Parks Road, University of Oxford, Oxford OX1 3QX, United Kingdom
§ Department of Physics, University of Oxford, Parks Road, Oxford OX1 3PU, United Kingdom
[email protected]
Cite this: J. Am. Chem. Soc. 2011, 133, 24, 9370–9375
Publication Date (Web):May 18, 2011
https://doi.org/10.1021/ja200128n
Copyright © 2011 American Chemical Society
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    Abstract

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    The ability to routinely study eukaryotic ion channels in a synthetic lipid environment would have a major impact on our understanding of how different lipids influence ion channel function. Here, we describe a straightforward, detergent-free method for the in vitro reconstitution of eukaryotic ion channels and ionotropic receptors into droplet interface bilayers and measure their electrical activity at both the macroscopic and single-channel level. We explore the general applicability of this method by reconstitution of channels from a wide range of sources including recombinant cell lines and native tissues, as well as preparations that are difficult to study by conventional methods including erythrocytes and mitochondria.

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    Additional description of the reconstitution method, and further electrical characterization of ion channels present in this manuscript are included as Supporting Information. This information is available free of charge via the Internet at http://pubs.acs.org/.

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