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Multivalent, High-Relaxivity MRI Contrast Agents Using Rigid Cysteine-Reactive Gadolinium Complexes
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    Multivalent, High-Relaxivity MRI Contrast Agents Using Rigid Cysteine-Reactive Gadolinium Complexes
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    Department of Chemistry, University of California, Berkeley, California 94720-1460, United States
    Materials Sciences Division, Lawrence Berkeley National Laboratories, Berkeley, California 94720-1460, United States
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    Journal of the American Chemical Society

    Cite this: J. Am. Chem. Soc. 2011, 133, 37, 14704–14709
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    https://doi.org/10.1021/ja204516p
    Published July 29, 2011
    Copyright © 2011 American Chemical Society

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    MRI contrast agents providing very high relaxivity values can be obtained through the attachment of multiple gadolinium(III) complexes to the interior surfaces of genome-free viral capsids. In previous studies, the contrast enhancement was predicted to depend on the rigidity of the linker attaching the MRI agents to the protein surface. To test this hypothesis, a new set of Gd-hydroxypyridonate based MRI agents was prepared and attached to genetically introduced cysteine residues through flexible and rigid linkers. Greater contrast enhancements were seen for MRI agents that were attached via rigid linkers, validating the design concept and outlining a path for future improvements of nanoscale MRI contrast agents.

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    Full synthetic procedures are provided, as are descriptions of the analysis techniques. A summary of the molecular modeling techniques is also included. This material is available free of charge via the Internet at http://pubs.acs.org.

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    Cite this: J. Am. Chem. Soc. 2011, 133, 37, 14704–14709
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    Published July 29, 2011
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