Development of a Far-Red to Near-Infrared Fluorescence Probe for Calcium Ion and its Application to Multicolor Neuronal ImagingClick to copy article linkArticle link copied!
- Takahiro Egawa
- Kenjiro Hanaoka
- Yuichiro Koide
- Sakiko Ujita
- Naoya Takahashi
- Yuji Ikegaya
- Norio Matsuki
- Takuya Terai
- Tasuku Ueno
- Toru Komatsu
- Tetsuo Nagano
Abstract

To improve optical imaging of Ca2+ and to make available a distinct color window for multicolor imaging, we designed and synthesized CaSiR-1, a far-red to near-infrared fluorescence probe for Ca2+, using Si-rhodamine (SiR) as the fluorophore and the well-known Ca2+ chelator BAPTA. This wavelength region is advantageous, affording higher tissue penetration, lower background autofluorescence, and lower phototoxicity in comparison with the UV to visible range. CaSiR-1 has a high fluorescence off/on ratio of over 1000. We demonstrate its usefulness for multicolor fluorescence imaging of action potentials (visualized as increases in intracellular Ca2+) in brain slices loaded with sulforhodamine 101 (red color; specific for astrocytes) that were prepared from transgenic mice in which some neurons expressed green fluorescent protein.
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