ACS Publications. Most Trusted. Most Cited. Most Read
My Activity
CONTENT TYPES

Figure 1Loading Img

Real-Time Monitoring of Cell Apoptosis and Drug Screening Using Fluorescent Light-Up Probe with Aggregation-Induced Emission Characteristics

View Author Information
Department of Chemical and Biomolecular Engineering, 4 Engineering Drive 4, National University of Singapore, Singapore 117576
# Department of Chemistry, Institute for Advanced Study, Division of Biomedical Engineering, State Key Laboratory of Molecular Neuroscience and Institute of Molecular Functional Materials, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
Guangdong Innovative Research Team, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou 510640, China
§ Institute of Materials Research Engineering, 3 Research Link, Singapore 117602
Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore 637371
Cite this: J. Am. Chem. Soc. 2012, 134, 43, 17972–17981
Publication Date (Web):October 8, 2012
https://doi.org/10.1021/ja3064588
Copyright © 2012 American Chemical Society

    Article Views

    15035

    Altmetric

    -

    Citations

    LEARN ABOUT THESE METRICS
    Other access options
    Supporting Info (2)»

    Abstract

    Abstract Image

    Real-time monitoring of cell apoptosis could provide valuable insights into early detection of therapy efficiency and evaluation of disease progression. In this work, we designed and synthesized a new live-cell-permeable, fluorescent light-up probe for real-time cell apoptosis imaging. The probe is comprised of a hydrophilic caspase-specific Asp-Glu-Val-Asp (DEVD) peptide and a hydrophobic tetraphenylethene (TPE) unit, a typical fluorogen with aggregation-induced emission characteristics. In aqueous solution, the probe is almost nonfluorescent but displays significant fluorescence enhancement in response to caspase-3/-7, which are activated in the apoptotic process and able to cleave the DEVD moieties. This fluorescence “turn-on” response is ascribed to aggregation of cleaved hydrophobic TPE residues, which restricts the intramolecular rotations of TPE phenyl rings and populates the radiative decay channels. The light-up nature of the probe allows real-time monitoring of caspase-3/-7 activities both in solutions and in living cells with a high signal-to-noise ratio. The probe provides a new opportunity to screen enzyme inhibitors and evaluate the apoptosis-associated drug efficacy.

    Read this article

    To access this article, please review the available access options below.

    Get instant access

    Purchase Access

    Read this article for 48 hours. Check out below using your ACS ID or as a guest.

    Recommended

    Access through Your Institution

    You may have access to this article through your institution.

    Your institution does not have access to this content. You can change your affiliated institution below.

    Supporting Information

    ARTICLE SECTIONS
    Jump To

    Experimental procedures for intermediates; structural characterization data for TPE-N3 and Ac-DEVDK-TPE; particle size distribution of TPE-N3 and K-TPE; absorption spectra of TPE-N3 and Ac-DEVDK-TPE; movie showing the dynamic process of cell apoptosis imaging. This material is available free of charge via the Internet at http://pubs.acs.org.

    Terms & Conditions

    Most electronic Supporting Information files are available without a subscription to ACS Web Editions. Such files may be downloaded by article for research use (if there is a public use license linked to the relevant article, that license may permit other uses). Permission may be obtained from ACS for other uses through requests via the RightsLink permission system: http://pubs.acs.org/page/copyright/permissions.html.

    Cited By

    This article is cited by 535 publications.

    1. Chen Chen, Yi-Xin Chen, Chong-Jing Zhang. A Radical-Generating Probe to Release Free Fluorophores and Identify Artemisinin-Sensitive Cancer Cells. ACS Sensors 2024, 9 (5) , 2310-2316. https://doi.org/10.1021/acssensors.4c00010
    2. Antonia I. Antoniou, Michela Pesenti, Stefania Crespi, Dhriti S. Shenoy, Marta Penconi, Alberto Bossi, Sara Pellegrino. Aggregation-Induced Enhanced Emission of Tetraphenylethene-phenylalanine Hybrids: Synthesis and Characterization. The Journal of Organic Chemistry 2024, 89 (7) , 4733-4740. https://doi.org/10.1021/acs.joc.3c02969
    3. Chun Dai, Wei Ge, Tianyu Li, Xiuqi Kong, Minggang Tian, Jie Niu. Single Fluorescent Probe for Multiple Tasks: Illuminating Lipid Droplets and Lysosomes in Dual Channels and Distinguishing Autophagy and Apoptosis. Analytical Chemistry 2024, 96 (10) , 4013-4022. https://doi.org/10.1021/acs.analchem.3c03653
    4. Wei He, Ryan Tsz Kin Kwok, Zijie Qiu, Zheng Zhao, Ben Zhong Tang. A Holistic Perspective on Living Aggregate. Journal of the American Chemical Society 2024, 146 (8) , 5030-5044. https://doi.org/10.1021/jacs.3c09892
    5. Jayanta Dolai, Reeddhi Ray, Santu Ghosh, Anupam Maity, Nikhil R. Jana. Optical Nanomaterials for Advanced Bioimaging Applications. ACS Applied Optical Materials 2024, 2 (1) , 1-14. https://doi.org/10.1021/acsaom.3c00357
    6. Xiaoyi Meng, Yue Cheng, Fang Wang, Zhaogang Sun, Hongqian Chu, Yong Wang. Nano Self-Assembly for Apoptosis Induction and Early Therapeutic Efficacy Monitoring. Analytical Chemistry 2023, 95 (38) , 14421-14429. https://doi.org/10.1021/acs.analchem.3c02860
    7. Qishu Jiao, Yaxin Zheng, Shicheng Pei, Xuan Luo, Xiaoxing Wu, Keming Xu, Wenying Zhong. Cascade-Activated AIEgen-Peptide Probe for Noninvasively Monitoring Chymotrypsin-like Activity of Proteasomes in Cancer Cells. Analytical Chemistry 2023, 95 (23) , 9097-9106. https://doi.org/10.1021/acs.analchem.3c01589
    8. Yang Lu, Wen Sun, Jianjun Du, Jiangli Fan, Xiaojun Peng. Immuno-photodynamic Therapy (IPDT): Organic Photosensitizers and Their Application in Cancer Ablation. JACS Au 2023, 3 (3) , 682-699. https://doi.org/10.1021/jacsau.2c00591
    9. Yong Cheng, Alex E. Clark, Wonjun Yim, Raina M. Borum, Yu-Ci Chang, Zhicheng Jin, Tengyu He, Aaron F. Carlin, Jesse V. Jokerst. Protease-Responsive Potential-Tunable AIEgens for Cell Selective Imaging of TMPRSS2 and Accurate Inhibitor Screening. Analytical Chemistry 2023, 95 (7) , 3789-3798. https://doi.org/10.1021/acs.analchem.2c04988
    10. Jie Niu, Fangfang Meng, Qiuhua Hao, Chong Zong, Jinyu Fu, Haiyan Xue, Minggang Tian, Xiaoqiang Yu. Ratiometric and Discriminative Visualization of Autophagy and Apoptosis with a Single Fluorescent Probe Based on the Aggregation/Monomer Principle. Analytical Chemistry 2022, 94 (51) , 17885-17894. https://doi.org/10.1021/acs.analchem.2c03815
    11. Yunfei Zuo, Hanchen Shen, Feiyi Sun, Pei Li, Jianwei Sun, Ryan T. K. Kwok, Jacky W. Y. Lam, Ben Zhong Tang. Aggregation-Induced Emission Luminogens for Cell Death Research. ACS Bio & Med Chem Au 2022, 2 (3) , 236-257. https://doi.org/10.1021/acsbiomedchemau.1c00066
    12. Jun Liu, Hongyu Chen, Bing Wang, Yingping Luo, Guoqiang Yang, Shilu Zhang, Shayu Li. Triarylboron-Based High Photosensitive Probes for Apoptosis Detection, Tumor-Targeted Imaging, and Selectively Inducing Apoptosis of Tumor Cells by Photodynamics. Analytical Chemistry 2022, 94 (23) , 8483-8488. https://doi.org/10.1021/acs.analchem.2c01364
    13. Jianguo Lin, Dingyao Gao, Shijie Wang, Gaochao Lv, Xiuting Wang, Chunmei Lu, Ying Peng, Ling Qiu. Stimuli-Responsive Macrocyclization Scaffold Allows In Situ Self-Assembly of Radioactive Tracers for Positron Emission Tomography Imaging of Enzyme Activity. Journal of the American Chemical Society 2022, 144 (17) , 7667-7675. https://doi.org/10.1021/jacs.1c12935
    14. Jing Fang, Yan Zhao, Anna Wang, Yuqi Zhang, Chaoxiang Cui, Shuyue Ye, Qiulian Mao, Yali Feng, Jiachen Li, Chenjie Xu, Haibin Shi. In Vivo Quantitative Assessment of a Radiation Dose Based on Ratiometric Photoacoustic Imaging of Tumor Apoptosis. Analytical Chemistry 2022, 94 (12) , 5149-5158. https://doi.org/10.1021/acs.analchem.2c00098
    15. Meng Yuan, Xiao Fang, Ying Wu, Yuanji Xu, Hongjuan Feng, Jing Mu, Zhongxiang Chen, Yuhong Lin, Qinrui Fu, Wei Du, Huanghao Yang, Jibin Song. Activatable Nanoprobe with Aggregation-Induced Dual Fluorescence and Photoacoustic Signal Enhancement for Tumor Precision Imaging and Radiotherapy. Analytical Chemistry 2022, 94 (12) , 5204-5211. https://doi.org/10.1021/acs.analchem.2c00340
    16. Anna Tokarenko, Vladimír Nosek, Jiří Míšek. Design, Synthesis, and Evaluation of Probes for Spatially Resolved Imaging of Enantioselective Sulfoxide Reductases. The Journal of Organic Chemistry 2022, 87 (2) , 1585-1588. https://doi.org/10.1021/acs.joc.1c02669
    17. Haozhen Li, Jie Luo, Zhi-Yin Zhang, Rong-Jia Wei, Mo Xie, Yong-Liang Huang, Guo-Hong Ning, Dan Li. Cyclic Trinuclear Copper(I) Complex Exhibiting Aggregation-Induced Emission: A Novel Fluorescent Probe for the Selective Detection of Gold(III) Ions. Inorganic Chemistry 2022, 61 (1) , 414-421. https://doi.org/10.1021/acs.inorgchem.1c03049
    18. Ke Li, Tian-Bing Ren, Shuangyan Huan, Lin Yuan, Xiao-Bing Zhang. Progress and Perspective of Solid-State Organic Fluorophores for Biomedical Applications. Journal of the American Chemical Society 2021, 143 (50) , 21143-21160. https://doi.org/10.1021/jacs.1c10925
    19. Thanh Chung Pham, Van-Nghia Nguyen, Yeonghwan Choi, Songyi Lee, Juyoung Yoon. Recent Strategies to Develop Innovative Photosensitizers for Enhanced Photodynamic Therapy. Chemical Reviews 2021, 121 (21) , 13454-13619. https://doi.org/10.1021/acs.chemrev.1c00381
    20. Jianhui Weng, Yuqi Wang, Yan Zhang, Deju Ye. An Activatable Near-Infrared Fluorescence Probe for in Vivo Imaging of Acute Kidney Injury by Targeting Phosphatidylserine and Caspase-3. Journal of the American Chemical Society 2021, 143 (43) , 18294-18304. https://doi.org/10.1021/jacs.1c08898
    21. Mehwish Qayyum, Tayyaba Bushra, Zulfiqar Ali Khan, Hira Gul, Shumaila Majeed, Cong Yu, Umar Farooq, Ahson Jabbar Shaikh, Sohail Anjum Shahzad. Synthesis and Tetraphenylethylene-Based Aggregation-Induced Emission Probe for Rapid Detection of Nitroaromatic Compounds in Aqueous Media. ACS Omega 2021, 6 (39) , 25447-25460. https://doi.org/10.1021/acsomega.1c03439
    22. José L. Belmonte-Vázquez, Yoarhy A. Amador-Sánchez, Lizbeth A. Rodríguez-Cortés, Braulio Rodríguez-Molina. Dual-State Emission (DSE) in Organic Fluorophores: Design and Applications. Chemistry of Materials 2021, 33 (18) , 7160-7184. https://doi.org/10.1021/acs.chemmater.1c02460
    23. Yi Tao, Lin Chen, Meiling Pan, Fei Zhu, Dong Zhu. Tailored Biosensors for Drug Screening, Efficacy Assessment, and Toxicity Evaluation. ACS Sensors 2021, 6 (9) , 3146-3162. https://doi.org/10.1021/acssensors.1c01600
    24. Lei He, Shuyue Ye, Jing Fang, Yuqi Zhang, Chaoxiang Cui, Anna Wang, Yan Zhao, Haibin Shi. Real-Time Visualization of Embryonic Apoptosis Using a Near-Infrared Fluorogenic Probe for Embryo Development Evaluation. Analytical Chemistry 2021, 93 (35) , 12122-12130. https://doi.org/10.1021/acs.analchem.1c02793
    25. Emma Olson, Justin S. Plaut, Sarah A. Barnhill, Sinan Sabuncu, Corey M. Dambacher, Sean D. Speese, Srivathsan V. Ranganathan, Bruce P. Branchaud, Adem Yildirim. Enzyme-Instructed Formation of β-Sheet-Rich Nanoplatelets for Label-Free Protease Sensing. ACS Applied Nano Materials 2021, 4 (8) , 7800-7810. https://doi.org/10.1021/acsanm.1c01098
    26. Xuewen Zhang, Yao Chen, Cong Li, Zhijun Xue, Hongshan Wu, Jun Li, Hanlin Ou, Jing Shen, Dan Ding. Root Canal Disinfection Using Highly Effective Aggregation-Induced Emission Photosensitizer. ACS Applied Bio Materials 2021, 4 (5) , 3796-3804. https://doi.org/10.1021/acsabm.0c01274
    27. Germain Kwek, Thang Cong Do, Xiaoling Lu, Jun Lin, Bengang Xing. Scratching the Surface of Unventured Possibilities with In Situ Self-Assembly: Protease-Activated Developments for Imaging and Therapy. ACS Applied Bio Materials 2021, 4 (3) , 2192-2216. https://doi.org/10.1021/acsabm.0c01340
    28. Yulong Jin, Kun Xu, Yanyan Huang, Huifei Zhong, Rui Zhao. Activity-Based Probe for Ratiometric Fluorescence Imaging of Caspase-3 in Living Cells. Analytical Chemistry 2021, 93 (4) , 2045-2052. https://doi.org/10.1021/acs.analchem.0c03762
    29. Jia Yang, Hong-Wei An, Hao Wang. Self-Assembled Peptide Drug Delivery Systems. ACS Applied Bio Materials 2021, 4 (1) , 24-46. https://doi.org/10.1021/acsabm.0c00707
    30. Wenjun Qin, Yu Wu, Yunhong Hu, Yanming Dong, Tonghui Hao, Cheng Zhang. TPE-Based Peptide Micelles for Targeted Tumor Therapy and Apoptosis Monitoring. ACS Applied Bio Materials 2021, 4 (1) , 1038-1044. https://doi.org/10.1021/acsabm.0c01493
    31. Yuyao Li, Chenghong Xue, Zhijun Fang, Weipan Xu, Hexin Xie. In Vivo Visualization of γ-Glutamyl Transpeptidase Activity with an Activatable Self-Immobilizing Near-Infrared Probe. Analytical Chemistry 2020, 92 (22) , 15017-15024. https://doi.org/10.1021/acs.analchem.0c02954
    32. Da Hye Kim, Dong Wook Kim, June Young Jang, Nahyun Lee, Yoon-Joo Ko, Sang Moon Lee, Hae Jin Kim, Kun Na, Seung Uk Son. Fe3O4@Void@Microporous Organic Polymer-Based Multifunctional Drug Delivery Systems: Targeting, Imaging, and Magneto-Thermal Behaviors. ACS Applied Materials & Interfaces 2020, 12 (33) , 37628-37636. https://doi.org/10.1021/acsami.0c12237
    33. Maixian Liu, Bobo Gu, Wenbo Wu, Yukun Duan, Hongji Liu, Xiangquan Deng, Miaozhuang Fan, Xiaomei Wang, Xunbin Wei, Ken-Tye Yong, Ke Wang, Gaixia Xu, Bin Liu. Binary Organic Nanoparticles with Bright Aggregation-Induced Emission for Three-Photon Brain Vascular Imaging. Chemistry of Materials 2020, 32 (15) , 6437-6443. https://doi.org/10.1021/acs.chemmater.0c01577
    34. Norifumi Yamamoto. Free Energy Profile Analysis for the Aggregation-Induced Emission of Diphenyldibenzofulvene. The Journal of Physical Chemistry A 2020, 124 (24) , 4939-4945. https://doi.org/10.1021/acs.jpca.0c03240
    35. Kotoe Nakasha, Gaku Fukuhara. Aggregation-Induced Emission-Based Polymer Materials: Ratiometric Fluorescence Responses Controlled by Hydrostatic Pressure. ACS Applied Polymer Materials 2020, 2 (6) , 2303-2310. https://doi.org/10.1021/acsapm.0c00272
    36. Chong Chen, Lin-Lin Yang, A-Ling Tang, Pei-Yi Wang, Rong Dong, Zhi-Bing Wu, Zhong Li, Song Yang. Curcumin–Cu(II) Ensemble-Based Fluorescence “Turn-On” Mode Sensing the Plant Defensive Hormone Salicylic Acid In Situ and In Vivo. Journal of Agricultural and Food Chemistry 2020, 68 (17) , 4844-4850. https://doi.org/10.1021/acs.jafc.0c01283
    37. Jin-Biao Jiao, Guan-Zhen Wang, Xi-Le Hu, Yi Zang, Stéphane Maisonneuve, Adam C. Sedgwick, Jonathan L. Sessler, Juan Xie, Jia Li, Xiao-Peng He, He Tian. Cyclodextrin-Based Peptide Self-Assemblies (Spds) That Enhance Peptide-Based Fluorescence Imaging and Antimicrobial Efficacy. Journal of the American Chemical Society 2020, 142 (4) , 1925-1932. https://doi.org/10.1021/jacs.9b11207
    38. Sonashree Saxena, Anu Pradeep, Manickam Jayakannan. Enzyme-Responsive Theranostic FRET Probe Based on l-Aspartic Amphiphilic Polyester Nanoassemblies for Intracellular Bioimaging in Cancer Cells. ACS Applied Bio Materials 2019, 2 (12) , 5245-5262. https://doi.org/10.1021/acsabm.9b00450
    39. Fan Xia, Jun Wu, Xia Wu, Qinyu Hu, Jun Dai, Xiaoding Lou. Modular Design of Peptide- or DNA-Modified AIEgen Probes for Biosensing Applications. Accounts of Chemical Research 2019, 52 (11) , 3064-3074. https://doi.org/10.1021/acs.accounts.9b00348
    40. Kenry, Kok Chan Chong, Bin Liu. Reactivity-Based Organic Theranostic Bioprobes. Accounts of Chemical Research 2019, 52 (11) , 3051-3063. https://doi.org/10.1021/acs.accounts.9b00356
    41. Feiyi Wang, Sisi Hu, Qi Sun, Qiang Fei, Chao Ma, Cuifen Lu, Junqi Nie, Zuxing Chen, Jun Ren, Guo-Rong Chen, Guichun Yang, Xiao-Peng He, Tony D James. A Leucine Aminopeptidase-Activated Theranostic Prodrug for Cancer Diagnosis and Chemotherapy. ACS Applied Bio Materials 2019, 2 (11) , 4904-4910. https://doi.org/10.1021/acsabm.9b00655
    42. Qiang Li, Xinkai Qiao, Fengchao Wang, Xuejing Li, Jie Yang, Yang Liu, Linqi Shi, Dingbin Liu. Encapsulating a Single Nanoprobe in a Multifunctional Nanogel for High-Fidelity Imaging of Caspase Activity in Vivo. Analytical Chemistry 2019, 91 (21) , 13633-13638. https://doi.org/10.1021/acs.analchem.9b02834
    43. Dong Wang, Ben Zhong Tang. Aggregation-Induced Emission Luminogens for Activity-Based Sensing. Accounts of Chemical Research 2019, 52 (9) , 2559-2570. https://doi.org/10.1021/acs.accounts.9b00305
    44. Jia Wang, Tianwen Bai, Yue Chen, Canbin Ye, Ting Han, Anjun Qin, Jun Ling, Ben Zhong Tang. Palladium/Benzoic Acid-Catalyzed Regio- and Stereoselective Polymerization of Internal Diynes and Diols through C(sp3)–H Activation. ACS Macro Letters 2019, 8 (9) , 1068-1074. https://doi.org/10.1021/acsmacrolett.9b00448
    45. Minggang Tian, Yanyan Ma, Weiying Lin. Fluorescent Probes for the Visualization of Cell Viability. Accounts of Chemical Research 2019, 52 (8) , 2147-2157. https://doi.org/10.1021/acs.accounts.9b00289
    46. Minggang Tian, Jie Sun, Baoli Dong, Weiying Lin. Unique pH-Sensitive RNA Binder for Ratiometric Visualization of Cell Apoptosis. Analytical Chemistry 2019, 91 (15) , 10056-10063. https://doi.org/10.1021/acs.analchem.9b01959
    47. Qinghua Guan, Nan Li, Leilei Shi, Chunyang Yu, Xihui Gao, Jiapei Yang, Yuanyuan Guo, Peiyong Li, Xinyuan Zhu. Aggregation-Induced Emission Fluorophore-Based Molecular Beacon for Differentiating Tumor and Normal Cells by Detecting the Specific and False-Positive Signals. ACS Biomaterials Science & Engineering 2019, 5 (7) , 3618-3630. https://doi.org/10.1021/acsbiomaterials.9b00627
    48. Jinming Li, Chris Wai Tung Leung, Dexter Siu Hong Wong, Jianbin Xu, Rui Li, Yueyue Zhao, Chris Yu Yee Yung, Engui Zhao, Ben Zhong Tang, Liming Bian. Photocontrolled SiRNA Delivery and Biomarker-Triggered Luminogens of Aggregation-Induced Emission by Up-Conversion NaYF4:Yb3+Tm3+@SiO2 Nanoparticles for Inducing and Monitoring Stem-Cell Differentiation. ACS Applied Materials & Interfaces 2019, 11 (25) , 22074-22084. https://doi.org/10.1021/acsami.7b00845
    49. Ling Yin, Hao Sun, Meng Zhao, Anna Wang, Shanshan Qiu, Yinjia Gao, Jianan Ding, Shun-Jun Ji, Haibin Shi, Mingyuan Gao. Rational Design and Synthesis of a Metalloproteinase-Activatable Probe for Dual-Modality Imaging of Metastatic Lymph Nodes in Vivo. The Journal of Organic Chemistry 2019, 84 (10) , 6126-6133. https://doi.org/10.1021/acs.joc.9b00331
    50. Xiaojun Liu, Xiaoxiao Song, Dongrui Luan, Bo Hu, Kehua Xu, Bo Tang. Real-Time in Situ Visualizing of the Sequential Activation of Caspase Cascade Using a Multicolor Gold–Selenium Bonding Fluorescent Nanoprobe. Analytical Chemistry 2019, 91 (9) , 5994-6002. https://doi.org/10.1021/acs.analchem.9b00452
    51. Hao Li, Giacomo Parigi, Claudio Luchinat, Thomas J. Meade. Bimodal Fluorescence-Magnetic Resonance Contrast Agent for Apoptosis Imaging. Journal of the American Chemical Society 2019, 141 (15) , 6224-6233. https://doi.org/10.1021/jacs.8b13376
    52. Tianfu Zhang, Yuanyuan Li, Zheng Zheng, Ruquan Ye, Yiru Zhang, Ryan T. K. Kwok, Jacky W. Y. Lam, Ben Zhong Tang. In Situ Monitoring Apoptosis Process by a Self-Reporting Photosensitizer. Journal of the American Chemical Society 2019, 141 (14) , 5612-5616. https://doi.org/10.1021/jacs.9b00636
    53. Ling Yin, Hao Sun, Hao Zhang, Lei He, Ling Qiu, Jianguo Lin, Huawei Xia, Yuqi Zhang, Shunjun Ji, Haibin Shi, Mingyuan Gao. Quantitatively Visualizing Tumor-Related Protease Activity in Vivo Using a Ratiometric Photoacoustic Probe. Journal of the American Chemical Society 2019, 141 (7) , 3265-3273. https://doi.org/10.1021/jacs.8b13628
    54. Soichi Yokoyama, Nagatoshi Nishiwaki. Fluorescence Behavior of Bis(cyanostyryl)pyrrole Derivatives Depending on the Substituent Position of Cyano Groups in Solution and in Solid State. The Journal of Organic Chemistry 2019, 84 (3) , 1192-1200. https://doi.org/10.1021/acs.joc.8b02517
    55. Jianlei Shen, Yiru Zhang, Rong Hu, Ryan T. K. Kwok, Zhiming Wang, Anjun Qin, Ben Zhong Tang. Dual-Mode Ultrasensitive Detection of Nucleic Acids via an Aqueous “Seesaw” Strategy by Combining Aggregation-Induced Emission and Plasmonic Colorimetry. ACS Applied Nano Materials 2019, 2 (1) , 163-169. https://doi.org/10.1021/acsanm.8b01773
    56. Chunlei Zhu, Ryan T. K. Kwok, Jacky W. Y. Lam, Ben Zhong Tang. Aggregation-Induced Emission: A Trailblazing Journey to the Field of Biomedicine. ACS Applied Bio Materials 2018, 1 (6) , 1768-1786. https://doi.org/10.1021/acsabm.8b00600
    57. Wei Liu, Haitong Liu, Xiaoran Peng, Guoqiang Zhou, Dandan Liu, Shenghui Li, Jinchao Zhang, Shuxiang Wang. Hypoxia-Activated Anticancer Prodrug for Bioimaging, Tracking Drug Release, and Anticancer Application. Bioconjugate Chemistry 2018, 29 (10) , 3332-3343. https://doi.org/10.1021/acs.bioconjchem.8b00511
    58. Ling-Hong Xiong, Xuewen He, Zheng Zhao, Ryan T. K. Kwok, Yu Xiong, Peng Fei Gao, Fan Yang, Yalan Huang, Herman H.-Y. Sung, Ian D. Williams, Jacky W. Y. Lam, Jinquan Cheng, Renli Zhang, Ben Zhong Tang. Ultrasensitive Virion Immunoassay Platform with Dual-Modality Based on a Multifunctional Aggregation-Induced Emission Luminogen. ACS Nano 2018, 12 (9) , 9549-9557. https://doi.org/10.1021/acsnano.8b05270
    59. Yeping Wu, Muhammad Naeem Nizam, Xiaokang Ding, Fu-Jian Xu. Rational Design of Peptide-Functionalized Poly(Methacrylic Acid) Brushes for On-Chip Detection of Protease Biomarkers. ACS Biomaterials Science & Engineering 2018, 4 (6) , 2018-2025. https://doi.org/10.1021/acsbiomaterials.7b00584
    60. Xuewen He, Zheng Zhao, Ling-Hong Xiong, Peng Fei Gao, Chen Peng, Rong Sheng Li, Yu Xiong, Zhi Li, Herman H.-Y. Sung, Ian D. Williams, Ryan T. K. Kwok, Jacky W. Y. Lam, Cheng Zhi Huang, Nan Ma, Ben Zhong Tang. Redox-Active AIEgen-Derived Plasmonic and Fluorescent Core@Shell Nanoparticles for Multimodality Bioimaging. Journal of the American Chemical Society 2018, 140 (22) , 6904-6911. https://doi.org/10.1021/jacs.8b02350
    61. Bartłomiej Sadowski, Khaled Hassanein, Barbara Ventura, Daniel T. Gryko. Tetraphenylethylenepyrrolo[3,2-b]pyrrole Hybrids as Solid-State Emitters: The Role of Substitution Pattern. Organic Letters 2018, 20 (11) , 3183-3186. https://doi.org/10.1021/acs.orglett.8b01011
    62. Ju Mei, Youhong Huang, He Tian. Progress and Trends in AIE-Based Bioprobes: A Brief Overview. ACS Applied Materials & Interfaces 2018, 10 (15) , 12217-12261. https://doi.org/10.1021/acsami.7b14343
    63. Qing-Lan Li, Duo Wang, Yuanzheng Cui, Zhiying Fan, Li Ren, Dongdong Li, Jihong Yu. AIEgen-Functionalized Mesoporous Silica Gated by Cyclodextrin-Modified CuS for Cell Imaging and Chemo-Photothermal Cancer Therapy. ACS Applied Materials & Interfaces 2018, 10 (15) , 12155-12163. https://doi.org/10.1021/acsami.7b14566
    64. Hao Lin, Haitao Yang, Shuai Huang, Fujia Wang, Dong-Mei Wang, Bin Liu, Yi-Da Tang, Chong-Jing Zhang. Caspase-1 Specific Light-Up Probe with Aggregation-Induced Emission Characteristics for Inhibitor Screening of Coumarin-Originated Natural Products. ACS Applied Materials & Interfaces 2018, 10 (15) , 12173-12180. https://doi.org/10.1021/acsami.7b14845
    65. Junjian Chen, Meng Gao, Lin Wang, Shiwu Li, Jingcai He, Anjun Qin, Li Ren, Yingjun Wang, Ben Zhong Tang. Aggregation-Induced Emission Probe for Study of the Bactericidal Mechanism of Antimicrobial Peptides. ACS Applied Materials & Interfaces 2018, 10 (14) , 11436-11442. https://doi.org/10.1021/acsami.7b18221
    66. Tsuyoshi Ueda, Tomonori Tamura, Itaru Hamachi. In Situ Construction of Protein-Based Semisynthetic Biosensors. ACS Sensors 2018, 3 (3) , 527-539. https://doi.org/10.1021/acssensors.7b00894
    67. Zhuoming Ma, Chen Liu, Na Niu, Zhijun Chen, Shujun Li, Shouxin Liu, Jian Li. Seeking Brightness from Nature: J-Aggregation-Induced Emission in Cellulolytic Enzyme Lignin Nanoparticles. ACS Sustainable Chemistry & Engineering 2018, 6 (3) , 3169-3175. https://doi.org/10.1021/acssuschemeng.7b03265
    68. Wen Song, Jing Kuang, Chu-Xin Li, Mingkang Zhang, Diwei Zheng, Xuan Zeng, Chuanjun Liu, and Xian-Zheng Zhang . Enhanced Immunotherapy Based on Photodynamic Therapy for Both Primary and Lung Metastasis Tumor Eradication. ACS Nano 2018, 12 (2) , 1978-1989. https://doi.org/10.1021/acsnano.7b09112
    69. Ruoyu Zhang, Simon H. P. Sung, Guangxue Feng, Chong-Jing Zhang, Kenry, Ben Zhong Tang, and Bin Liu . Aggregation-Induced Emission Probe for Specific Turn-On Quantification of Soluble Transferrin Receptor: An Important Disease Marker for Iron Deficiency Anemia and Kidney Diseases. Analytical Chemistry 2018, 90 (2) , 1154-1160. https://doi.org/10.1021/acs.analchem.7b03694
    70. Andrés Garzón, Amparo Navarro, Daniel López, Josefina Perles, and Eva M. García-Frutos . Aggregation-Induced Enhanced Emission (AIEE) from N,N-Octyl-7,7′-diazaisoindigo-Based Organogel. The Journal of Physical Chemistry C 2017, 121 (48) , 27071-27081. https://doi.org/10.1021/acs.jpcc.7b07625
    71. Xuesong Li, Meijuan Jiang, Jacky W. Y. Lam, Ben Zhong Tang, and Jianan Y. Qu . Mitochondrial Imaging with Combined Fluorescence and Stimulated Raman Scattering Microscopy Using a Probe of the Aggregation-Induced Emission Characteristic. Journal of the American Chemical Society 2017, 139 (47) , 17022-17030. https://doi.org/10.1021/jacs.7b06273
    72. Zhiling Zhang, Ryan T. K. Kwok, Yong Yu, Ben Zhong Tang, and Ka Ming Ng . Sensitive and Specific Detection of l-Lactate Using an AIE-Active Fluorophore. ACS Applied Materials & Interfaces 2017, 9 (44) , 38153-38158. https://doi.org/10.1021/acsami.7b10178
    73. Meng Gao and Ben Zhong Tang . Fluorescent Sensors Based on Aggregation-Induced Emission: Recent Advances and Perspectives. ACS Sensors 2017, 2 (10) , 1382-1399. https://doi.org/10.1021/acssensors.7b00551
    74. Tae-Il Kim, Hanyong Jin, Jeehyeon Bae, and Youngmi Kim . Excimer Emission-Based Fluorescent Probe Targeting Caspase-3. Analytical Chemistry 2017, 89 (19) , 10565-10569. https://doi.org/10.1021/acs.analchem.7b02790
    75. Sheng-Lin Qiao, Yang Ma, Yi Wang, Yao-Xin Lin, Hong-Wei An, Li-Li Li, and Hao Wang . General Approach of Stimuli-Induced Aggregation for Monitoring Tumor Therapy. ACS Nano 2017, 11 (7) , 7301-7311. https://doi.org/10.1021/acsnano.7b03375
    76. Xin Han, Jian Zhang, Chen-Yang Qiao, Wen-Ming Zhang, Jun Yin, and Zong-Quan Wu . High-Efficiency Cell-Penetrating Helical Poly(phenyl isocyanide) Chains Modified Cellular Tracer and Nanovectors with Thiol Ratiometric Fluorescence Imaging Performance. Macromolecules 2017, 50 (11) , 4114-4125. https://doi.org/10.1021/acs.macromol.7b00669
    77. Qin Huang, Jing Xie, Yanpeng Liu, Anna Zhou, and Jianshu Li . Detecting the Formation and Transformation of Oligomers during Insulin Fibrillation by a Dendrimer Conjugated with Aggregation-Induced Emission Molecule. Bioconjugate Chemistry 2017, 28 (4) , 944-956. https://doi.org/10.1021/acs.bioconjchem.6b00665
    78. Hong Cheng, Shi-Ying Li, Hao-Ran Zheng, Chu-Xin Li, Bo-Ru Xie, Ke-Wei Chen, Bin Li, and Xian-Zheng Zhang . Multi-Förster Resonance Energy Transfer-Based Fluorescent Probe for Spatiotemporal Matrix Metalloproteinase-2 and Caspase-3 Imaging. Analytical Chemistry 2017, 89 (8) , 4349-4354. https://doi.org/10.1021/acs.analchem.7b00277
    79. Yuan-Jun Gao, Xue-Ping Chang, Xiang-Yang Liu, Quan-Song Li, Ganglong Cui, and Walter Thiel . Excited-State Decay Paths in Tetraphenylethene Derivatives. The Journal of Physical Chemistry A 2017, 121 (13) , 2572-2579. https://doi.org/10.1021/acs.jpca.7b00197
    80. Beatriz Pelaz, Christoph Alexiou, Ramon A. Alvarez-Puebla, Frauke Alves, Anne M. Andrews, Sumaira Ashraf, Lajos P. Balogh, Laura Ballerini, Alessandra Bestetti, Cornelia Brendel, Susanna Bosi, Monica Carril, Warren C. W. Chan, Chunying Chen, Xiaodong Chen, Xiaoyuan Chen, Zhen Cheng, Daxiang Cui, Jianzhong Du, Christian Dullin, Alberto Escudero, Neus Feliu, Mingyuan Gao, Michael George, Yury Gogotsi, Arnold Grünweller, Zhongwei Gu, Naomi J. Halas, Norbert Hampp, Roland K. Hartmann, Mark C. Hersam, Patrick Hunziker, Ji Jian, Xingyu Jiang, Philipp Jungebluth, Pranav Kadhiresan, Kazunori Kataoka, Ali Khademhosseini, Jindřich Kopeček, Nicholas A. Kotov, Harald F. Krug, Dong Soo Lee, Claus-Michael Lehr, Kam W. Leong, Xing-Jie Liang, Mei Ling Lim, Luis M. Liz-Marzán, Xiaowei Ma, Paolo Macchiarini, Huan Meng, Helmuth Möhwald, Paul Mulvaney, Andre E. Nel, Shuming Nie, Peter Nordlander, Teruo Okano, Jose Oliveira, Tai Hyun Park, Reginald M. Penner, Maurizio Prato, Victor Puntes, Vincent M. Rotello, Amila Samarakoon, Raymond E. Schaak, Youqing Shen, Sebastian Sjöqvist, Andre G. Skirtach, Mahmoud G. Soliman, Molly M. Stevens, Hsing-Wen Sung, Ben Zhong Tang, Rainer Tietze, Buddhisha N. Udugama, J. Scott VanEpps, Tanja Weil, Paul S. Weiss, Itamar Willner, Yuzhou Wu, Lily Yang, Zhao Yue, Qian Zhang, Qiang Zhang, Xian-En Zhang, Yuliang Zhao, Xin Zhou, and Wolfgang J. Parak . Diverse Applications of Nanomedicine. ACS Nano 2017, 11 (3) , 2313-2381. https://doi.org/10.1021/acsnano.6b06040
    81. Yao-Xin Lin, Sheng-Lin Qiao, Yi Wang, Ruo-Xin Zhang, Hong-Wei An, Yang Ma, R. P. Yeshan J. Rajapaksha, Zeng-Ying Qiao, Lei Wang, and Hao Wang . An in Situ Intracellular Self-Assembly Strategy for Quantitatively and Temporally Monitoring Autophagy. ACS Nano 2017, 11 (2) , 1826-1839. https://doi.org/10.1021/acsnano.6b07843
    82. Guangxue Feng, Chong-Jing Zhang, Xianmao Lu, and Bin Liu . Zinc(II)-Tetradentate-Coordinated Probe with Aggregation-Induced Emission Characteristics for Selective Imaging and Photoinactivation of Bacteria. ACS Omega 2017, 2 (2) , 546-553. https://doi.org/10.1021/acsomega.6b00564
    83. Mengmeng Zheng, Yuqi Wang, Hua Shi, Yuxuan Hu, Liandong Feng, Zhiliang Luo, Mi Zhou, Jian He, Zhenyang Zhou, Yan Zhang, and Deju Ye . Redox-Mediated Disassembly to Build Activatable Trimodal Probe for Molecular Imaging of Biothiols. ACS Nano 2016, 10 (11) , 10075-10085. https://doi.org/10.1021/acsnano.6b05030
    84. Kaizhi Gu, Yajing Liu, Zhiqian Guo, Cheng Lian, Chenxu Yan, Ping Shi, He Tian, and Wei-Hong Zhu . In Situ Ratiometric Quantitative Tracing of Intracellular Leucine Aminopeptidase Activity via an Activatable Near-Infrared Fluorescent Probe. ACS Applied Materials & Interfaces 2016, 8 (40) , 26622-26629. https://doi.org/10.1021/acsami.6b10238
    85. Xiaofeng Fang, Yu-Mo Zhang, Kaiwen Chang, Zhihe Liu, Xing Su, Haobin Chen, Sean Xiao-An Zhang, Yifei Liu, and Changfeng Wu . Facile Synthesis, Macroscopic Separation, E/Z Isomerization, and Distinct AIE properties of Pure Stereoisomers of an Oxetane-Substituted Tetraphenylethene Luminogen. Chemistry of Materials 2016, 28 (18) , 6628-6636. https://doi.org/10.1021/acs.chemmater.6b02746
    86. Suman Pal, Vadde Ramu, Nandaraj Taye, Devraj G. Mogare, Amar M. Yeware, Dhiman Sarkar, D. Srinivasa Reddy, Samit Chattopadhyay, and Amitava Das . GSH Induced Controlled Release of Levofloxacin from a Purpose-Built Prodrug: Luminescence Response for Probing the Drug Release in Escherichia coli and Staphylococcus aureus. Bioconjugate Chemistry 2016, 27 (9) , 2062-2070. https://doi.org/10.1021/acs.bioconjchem.6b00324
    87. Ruoyu Zhang, Chong-Jing Zhang, Guangxue Feng, Fang Hu, Jigang Wang, and Bin Liu . Specific Light-Up Probe with Aggregation-Induced Emission for Facile Detection of Chymase. Analytical Chemistry 2016, 88 (18) , 9111-9117. https://doi.org/10.1021/acs.analchem.6b02073
    88. Yong Cheng, Fujian Huang, Xuehong Min, Pengcheng Gao, Tianchi Zhang, Xinchun Li, Bifeng Liu, Yuning Hong, Xiaoding Lou, and Fan Xia . Protease-Responsive Prodrug with Aggregation-Induced Emission Probe for Controlled Drug Delivery and Drug Release Tracking in Living Cells. Analytical Chemistry 2016, 88 (17) , 8913-8919. https://doi.org/10.1021/acs.analchem.6b02833
    89. Tengyan Xu, Chunhui Liang, Shenglu Ji, Dan Ding, Deling Kong, Ling Wang, and Zhimou Yang . Surface-Induced Hydrogelation for Fluorescence and Naked-Eye Detections of Enzyme Activity in Blood. Analytical Chemistry 2016, 88 (14) , 7318-7323. https://doi.org/10.1021/acs.analchem.6b01660
    90. Hongbo Yuan, Yibing Fan, Chengfen Xing, Ruimin Niu, Ran Chai, Yong Zhan, Junjie Qi, Hailong An, and Jialiang Xu . Conjugated Polymer-Based Hybrid Materials for Turn-On Detection of CO2 in Plant Photosynthesis. Analytical Chemistry 2016, 88 (12) , 6593-6597. https://doi.org/10.1021/acs.analchem.6b01489
    91. Zeng-Ying Qiao, Wen-Jing Zhao, Yong Cong, Di Zhang, Zhiyuan Hu, Zhong-Yu Duan, and Hao Wang . Self-Assembled ROS-Sensitive Polymer–Peptide Therapeutics Incorporating Built-in Reporters for Evaluation of Treatment Efficacy. Biomacromolecules 2016, 17 (5) , 1643-1652. https://doi.org/10.1021/acs.biomac.6b00041
    92. Young-Jae Jin, Hyojin Kim, Jong Jin Kim, Nam Ho Heo, Jong Won Shin, Masahiro Teraguchi, Takashi Kaneko, Toshiki Aoki, and Giseop Kwak . Asymmetric Restriction of Intramolecular Rotation in Chiral Solvents. Crystal Growth & Design 2016, 16 (5) , 2804-2809. https://doi.org/10.1021/acs.cgd.6b00128
    93. Ruoyu Zhang, Guangxue Feng, Chong-Jing Zhang, Xiaolei Cai, Xiamin Cheng, and Bin Liu . Real-Time Specific Light-Up Sensing of Transferrin Receptor: Image-Guided Photodynamic Ablation of Cancer Cells through Controlled Cytomembrane Disintegration. Analytical Chemistry 2016, 88 (9) , 4841-4848. https://doi.org/10.1021/acs.analchem.6b00524
    94. Adukkadan N. Ramya, Manu M. Joseph, Jyothi B. Nair, Varsha Karunakaran, Nisha Narayanan, and Kaustabh Kumar Maiti . New Insight of Tetraphenylethylene-based Raman Signatures for Targeted SERS Nanoprobe Construction Toward Prostate Cancer Cell Detection. ACS Applied Materials & Interfaces 2016, 8 (16) , 10220-10225. https://doi.org/10.1021/acsami.6b01908
    95. Xuehong Min, Mengshi Zhang, Fujian Huang, Xiaoding Lou, and Fan Xia . Live Cell MicroRNA Imaging Using Exonuclease III-Aided Recycling Amplification Based on Aggregation-Induced Emission Luminogens. ACS Applied Materials & Interfaces 2016, 8 (14) , 8998-9003. https://doi.org/10.1021/acsami.6b01581
    96. Aitian Han, Huaimin Wang, Ryan T. K. Kwok, Shenglu Ji, Jun Li, Deling Kong, Ben Zhong Tang, Bin Liu, Zhimou Yang, and Dan Ding . Peptide-Induced AIEgen Self-Assembly: A New Strategy to Realize Highly Sensitive Fluorescent Light-Up Probes. Analytical Chemistry 2016, 88 (7) , 3872-3878. https://doi.org/10.1021/acs.analchem.6b00023
    97. Lu Xiao, Zhaojuan Zhou, Mengli Feng, Aijun Tong, and Yu Xiang . Cationic Peptide Conjugation Enhances the Activity of Peroxidase-Mimicking DNAzymes. Bioconjugate Chemistry 2016, 27 (3) , 621-627. https://doi.org/10.1021/acs.bioconjchem.5b00608
    98. Kuheli Mandal, Debabrata Jana, Binay K. Ghorai, and Nikhil R. Jana . Fluorescent Imaging Probe from Nanoparticle Made of AIE Molecule. The Journal of Physical Chemistry C 2016, 120 (9) , 5196-5206. https://doi.org/10.1021/acs.jpcc.5b12682
    99. Ya-Guang He, Sheng-Yu Shi, Na Liu, Yun-Sheng Ding, Jun Yin, and Zong-Quan Wu . Tetraphenylethene-Functionalized Conjugated Helical Poly(phenyl isocyanide) with Tunable Light Emission, Assembly Morphology, and Specific Applications. Macromolecules 2016, 49 (1) , 48-58. https://doi.org/10.1021/acs.macromol.5b02412
    100. Yanbin Cai, Jie Zhan, Haosheng Shen, Duo Mao, Shenglu Ji, Ruihua Liu, Bing Yang, Deling Kong, Ling Wang, and Zhimou Yang . Optimized Ratiometric Fluorescent Probes by Peptide Self-Assembly. Analytical Chemistry 2016, 88 (1) , 740-745. https://doi.org/10.1021/acs.analchem.5b02955
    Load more citations

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    You’ve supercharged your research process with ACS and Mendeley!

    STEP 1:
    Click to create an ACS ID

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    MENDELEY PAIRING EXPIRED
    Your Mendeley pairing has expired. Please reconnect