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Absolute and Direct MicroRNA Quantification Using DNA–Gold Nanoparticle Probes

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    Absolute and Direct MicroRNA Quantification Using DNA–Gold Nanoparticle Probes
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    Center for Biomolecular Nanotechnologies@UniLe, Istituto Italiano di Tecnologia (IIT),Via Barsanti, 73010 Arnesano, Lecce, Italy
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    Journal of the American Chemical Society

    Cite this: J. Am. Chem. Soc. 2014, 136, 6, 2264–2267
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    https://doi.org/10.1021/ja412152x
    Published February 3, 2014
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    Abstract

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    DNA–gold nanoparticle probes are implemented in a simple strategy for direct microRNA (miRNA) quantification. Fluorescently labeled DNA-probe strands are immobilized on PEGylated gold nanoparticles (AuNPs). In the presence of target miRNA, DNA–RNA heteroduplexes are formed and become substrate for the endonuclease DSN (duplex-specific nuclease). Enzymatic hydrolysis of the DNA strands yields a fluorescence signal due to diffusion of the fluorophores away from the gold surface. We show that the molecular design of our DNA–AuNP probes, with the DNA strands immobilized on top of the PEG-based passivation layer, results in nearly unaltered enzymatic activity toward immobilized heteroduplexes compared to substrates free in solution. The assay, developed in a real-time format, allows absolute quantification of as little as 0.2 fmol of miR-203. We also show the application of the assay for direct quantification of cancer-related miR-203 and miR-21 in samples of extracted total RNA from cell cultures. The possibility of direct and absolute quantification may significantly advance the use of microRNAs as biomarkers in the clinical praxis.

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    73. Lei Ge, Wenxiao Wang, Ximei Sun, Ting Hou, and Feng Li . Affinity-Mediated Homogeneous Electrochemical Aptasensor on a Graphene Platform for Ultrasensitive Biomolecule Detection via Exonuclease-Assisted Target-Analog Recycling Amplification. Analytical Chemistry 2016, 88 (4) , 2212-2219. https://doi.org/10.1021/acs.analchem.5b03844
    74. Si Li, Liguang Xu, Wei Ma, Xiaoling Wu, Maozhong Sun, Hua Kuang, Libing Wang, Nicholas A. Kotov, and Chuanlai Xu . Dual-Mode Ultrasensitive Quantification of MicroRNA in Living Cells by Chiroplasmonic Nanopyramids Self-Assembled from Gold and Upconversion Nanoparticles. Journal of the American Chemical Society 2016, 138 (1) , 306-312. https://doi.org/10.1021/jacs.5b10309
    75. Richard M. Graybill and Ryan C. Bailey . Emerging Biosensing Approaches for microRNA Analysis. Analytical Chemistry 2016, 88 (1) , 431-450. https://doi.org/10.1021/acs.analchem.5b04679
    76. Yongxi Zhao, Feng Chen, Qian Li, Lihua Wang, and Chunhai Fan . Isothermal Amplification of Nucleic Acids. Chemical Reviews 2015, 115 (22) , 12491-12545. https://doi.org/10.1021/acs.chemrev.5b00428
    77. Wen Zhou, Xia Gao, Dingbin Liu, and Xiaoyuan Chen . Gold Nanoparticles for In Vitro Diagnostics. Chemical Reviews 2015, 115 (19) , 10575-10636. https://doi.org/10.1021/acs.chemrev.5b00100
    78. Feng Chen, Chunhai Fan, and Yongxi Zhao . Inhibitory Impact of 3′-Terminal 2′-O-Methylated Small Silencing RNA on Target-Primed Polymerization and Unbiased Amplified Quantification of the RNA in Arabidopsis thaliana. Analytical Chemistry 2015, 87 (17) , 8758-8764. https://doi.org/10.1021/acs.analchem.5b01683
    79. Sujuan Ye, Yanying Wu, Xiaomo Zhai, and Bo Tang . Asymmetric Signal Amplification for Simultaneous SERS Detection of Multiple Cancer Markers with Significantly Different Levels. Analytical Chemistry 2015, 87 (16) , 8242-8249. https://doi.org/10.1021/acs.analchem.5b01186
    80. Xuehong Min, Yuan Zhuang, Zhenyu Zhang, Yongmei Jia, Abdul Hakeem, Fuxin Zheng, Yong Cheng, Ben Zhong Tang, Xiaoding Lou, and Fan Xia . Lab in a Tube: Sensitive Detection of MicroRNAs in Urine Samples from Bladder Cancer Patients Using a Single-Label DNA Probe with AIEgens. ACS Applied Materials & Interfaces 2015, 7 (30) , 16813-16818. https://doi.org/10.1021/acsami.5b04821
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    82. Xiaopei Qiu, Xing Liu, Wei Zhang, Hong Zhang, Tianlun Jiang, Dongli Fan, and Yang Luo . Dynamic Monitoring of MicroRNA–DNA Hybridization Using DNAase-Triggered Signal Amplification. Analytical Chemistry 2015, 87 (12) , 6303-6310. https://doi.org/10.1021/acs.analchem.5b01159
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    84. Cuiyun Yang, Baoting Dou, Kai Shi, Yaqin Chai, Yun Xiang, and Ruo Yuan . Multiplexed and Amplified Electronic Sensor for the Detection of MicroRNAs from Cancer Cells. Analytical Chemistry 2014, 86 (23) , 11913-11918. https://doi.org/10.1021/ac503860d
    85. Haiyun Liu, Lu Li, Qian Wang, Lili Duan, and Bo Tang . Graphene Fluorescence Switch-Based Cooperative Amplification: A Sensitive and Accurate Method to Detection MicroRNA. Analytical Chemistry 2014, 86 (11) , 5487-5493. https://doi.org/10.1021/ac500752t
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    Cite this: J. Am. Chem. Soc. 2014, 136, 6, 2264–2267
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