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Sortase A-Catalyzed Transpeptidation of Glycosylphosphatidylinositol Derivatives for Chemoenzymatic Synthesis of GPI-Anchored Proteins

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Department of Chemistry, Wayne State University, 5101 Cass Avenue, Detroit, Michigan 48202
[email protected]
Cite this: J. Am. Chem. Soc. 2010, 132, 5, 1567–1571
Publication Date (Web):January 15, 2010
https://doi.org/10.1021/ja906611x
Copyright © 2010 American Chemical Society
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    Abstract

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    Several peptides/small proteins and glycosylphosphatidylinositol (GPI) derivatives were synthesized and compared as substrates of sortase A (SrtA), a bacterial transpeptidase, for enzymatic coupling. It was observed that peptides containing the LPKTGGS and LPKTGGRS sequences as sorting signals at the peptide C-terminus were effectively coupled to GPI derivatives having one or two glycine residues attached to the phosphoethanolamine group at the nonreducing end. This reaction was employed to prepare several analogues of the human CD52 and CD24 antigens, which are naturally GPI-anchored glycopeptides/glycoproteins. It was further observed that the trisaccharide GPI analogues 5 and 6 were better SrtA substrates than monosaccharide GPI analogue 4, suggesting that steric hindrance of the GPI analogues does not affect their peptidation reaction mediated by SrtA. Therefore, this synthetic strategy may be useful for the preparation of more complex GPI-anchored peptides, glycopeptides, proteins, and glycoproteins.

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    Experimental details for the preparation of SrtA and compounds 5−9 and 25; conditions and protocols for the enzymatic reactions of 4−6 with 7−9 and of 5 with 25 to prepare 10−15 and 26, respectively; HPLC results for the enzymatic reactions; and MS and NMR spectra of all intermediates and products. This material is available free of charge via the Internet at http://pubs.acs.org.

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