ACS Publications. Most Trusted. Most Cited. Most Read

OR SEARCH CITATIONS

My Activity
Recently Viewed
You have not visited any articles yet, Please visit some articles to see contents here.
Publications
CONTENT TYPES

Figure 1Loading Img
Download Hi-Res ImageDownload to MS-PowerPointCite This:J. Am. Chem. Soc. 2022, 144, 21, 9443-9450
RETURN TO ISSUEPREVArticleNEXT

Living Cell Nanoporation and Exosomal RNA Analysis Platform for Real-Time Assessment of Cellular Therapies

  • Yan Sheng
    Yan Sheng
    MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, People’s Republic of China
    More by Yan Sheng
    • , 
  • Zena Huang
    Zena Huang
    Department of General Medicine, Guangdong Provincial Geriatrics Institute, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, People’s Republic of China
    More by Zena Huang
    • , 
  • Tenghua Zhang
    Tenghua Zhang
    MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, People’s Republic of China
    More by Tenghua Zhang
    • , 
  • Feiyang Qian
    Feiyang Qian
    MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, People’s Republic of China
    More by Feiyang Qian
    • , 
  • Yuqing Zhu
    Yuqing Zhu
    MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, People’s Republic of China
    More by Yuqing Zhu
    • , 
  • Zaizai Dong
    Zaizai Dong
    Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, People’s Republic of China
    More by Zaizai Dong
    • , 
  • Qingyang Zhang
    Qingyang Zhang
    Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, People’s Republic of China
    More by Qingyang Zhang
    • , 
  • Qi Lei
    Qi Lei
    MOE International Joint Research Laboratory on Synthetic Biology and Medicines, School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, People’s Republic of China
    More by Qi Lei
    • , 
  • Fanhui Kong
    Fanhui Kong
    MOE International Joint Research Laboratory on Synthetic Biology and Medicines, School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, People’s Republic of China
    More by Fanhui Kong
    • , 
  • Yuqiong Wang
    Yuqiong Wang
    Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, People’s Republic of China
    More by Yuqiong Wang
    • , 
  • Maddie Walden
    Maddie Walden
    BCMaterials, Basque Center for Materials, UPV/EHU Science Park, 48940 Leioa, Spain
    More by Maddie Walden
    • , 
  • Stefan Wuttke
    Stefan Wuttke
    BCMaterials, Basque Center for Materials, UPV/EHU Science Park, 48940 Leioa, Spain
    Ikerbasque, Basque Foundation for Science, 48009 Bilbao, Spain
    More by Stefan Wuttke
    Orcidhttps://orcid.org/0000-0002-6344-5782
    • , 
  • Lingqian Chang*
    Lingqian Chang
    Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, People’s Republic of China
    *[email protected]
    More by Lingqian Chang
    Orcidhttps://orcid.org/0000-0002-6329-9850
    • , 
  • Wei Zhu*
    Wei Zhu
    MOE International Joint Research Laboratory on Synthetic Biology and Medicines, School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, People’s Republic of China
    *[email protected]
    More by Wei Zhu
    Orcidhttps://orcid.org/0000-0003-3240-6905
    • , and 
  • Jiaming Hu*
    Jiaming Hu
    MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, People’s Republic of China
    *[email protected]
    More by Jiaming Hu
    Orcidhttps://orcid.org/0000-0001-5028-3123
Cite this: J. Am. Chem. Soc. 2022, 144, 21, 9443–9450
Publication Date (Web):May 22, 2022
https://doi.org/10.1021/jacs.2c02268
Copyright © 2022 American Chemical Society
RIGHTS & PERMISSIONS
Article Views
2114
Altmetric
-
Citations
1
LEARN ABOUT THESE METRICS
Read OnlinePDF (6 MB)
Get e-Alerts
Supporting Info (1)»
SUBJECTS:

Abstract

Abstract Image

Efficient transfection of therapeutic agents and timely potency testing are two key factors hindering the development of cellular therapy. Here we present a cellular-nanoporation and exosome assessment device, a quantitative platform for nanochannel-based cell electroporation and exosome-based in situ RNA expression analysis. In its application to transfection of anti-miRNAs and/or chemotherapeutics into cells, we have systematically described the differences in RNA expression in secreted exosomes and assessed cellular therapies in real time.

Supporting Information

ARTICLE SECTIONS
Jump To

The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/jacs.2c02268.

  • Additional experimental details, materials, methods, and structure characterization data (PDF)

Terms & Conditions

Most electronic Supporting Information files are available without a subscription to ACS Web Editions. Such files may be downloaded by article for research use (if there is a public use license linked to the relevant article, that license may permit other uses). Permission may be obtained from ACS for other uses through requests via the RightsLink permission system: http://pubs.acs.org/page/copyright/permissions.html.

Cited By

This article is cited by 1 publications.

  1. Fengqi Wan, Zaizai Dong, Bing Liu, Shi Yan, Nan Wu, Mingzhu Yang, Lingqian Chang. Sensitive Interrogation of Enhancer Activity in Living Cells on a Nanoelectroporation-Probing Platform. ACS Sensors 2022, 7 (12) , 3671-3681. https://doi.org/10.1021/acssensors.2c01187

Pair your accounts.

Export articles to Mendeley

Get article recommendations from ACS based on references in your Mendeley library.

Pair your accounts.

Export articles to Mendeley

Get article recommendations from ACS based on references in your Mendeley library.

You’ve supercharged your research process with ACS and Mendeley!

STEP 1:
Click to create an ACS ID

Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

MENDELEY PAIRING EXPIRED
Your Mendeley pairing has expired. Please reconnect

This website uses cookies to improve your user experience. By continuing to use the site, you are accepting our use of cookies. Read the ACS privacy policy.

CONTINUE