Analysis of Erythroxylum coca Leaves by Imaging Mass Spectrometry (MALDI–FT–ICR IMS)Click to copy article linkArticle link copied!
- Nayara Araujo dos SantosNayara Araujo dos SantosLaboratório de Petroleômica e Forense, Universidade Federal do Espírito Santo (UFES), Avenida Fernando Ferrari, 514, Goiabeiras, Vitória, ES 29075-910, BrazilInstituto Nacional de Ciência e Tecnologia Forense (INCT Forense), Av. Ministro Salgado Filho, Soteco, Vila Velha, Espírito Santo 29106-010, BrazilMore by Nayara Araujo dos Santos
- Camila Medeiros de AlmeidaCamila Medeiros de AlmeidaLaboratório de Petroleômica e Forense, Universidade Federal do Espírito Santo (UFES), Avenida Fernando Ferrari, 514, Goiabeiras, Vitória, ES 29075-910, BrazilInstituto Nacional de Ciência e Tecnologia Forense (INCT Forense), Av. Ministro Salgado Filho, Soteco, Vila Velha, Espírito Santo 29106-010, BrazilMore by Camila Medeiros de Almeida
- Fernanda Fachim GonçalvesFernanda Fachim GonçalvesLaboratório de Petroleômica e Forense, Universidade Federal do Espírito Santo (UFES), Avenida Fernando Ferrari, 514, Goiabeiras, Vitória, ES 29075-910, BrazilMore by Fernanda Fachim Gonçalves
- Rafael Scorsatto OrtizRafael Scorsatto OrtizInstituto Nacional de Ciência e Tecnologia Forense (INCT Forense), Av. Ministro Salgado Filho, Soteco, Vila Velha, Espírito Santo 29106-010, BrazilSuperintendência da Polícia Federal no Rio Grande Sul, Porto Alegre, RS 90040-410, BrazilMore by Rafael Scorsatto Ortiz
- Ricardo Machado KusterRicardo Machado KusterLaboratório de Petroleômica e Forense, Universidade Federal do Espírito Santo (UFES), Avenida Fernando Ferrari, 514, Goiabeiras, Vitória, ES 29075-910, BrazilMore by Ricardo Machado Kuster
- Diemerson SaquettoDiemerson SaquettoInstituto Federal de Educação, Ciência e Tecnologia do Espírito Santo (IFES), Av. Ministro Salgado Filho, Soteco, Vila Velha, Espírito Santo 29106-010, BrazilMore by Diemerson Saquetto
- Wanderson Romão*Wanderson Romão*Tel: +55 02740094512. Email: [email protected]Laboratório de Petroleômica e Forense, Universidade Federal do Espírito Santo (UFES), Avenida Fernando Ferrari, 514, Goiabeiras, Vitória, ES 29075-910, BrazilInstituto Nacional de Ciência e Tecnologia Forense (INCT Forense), Av. Ministro Salgado Filho, Soteco, Vila Velha, Espírito Santo 29106-010, BrazilInstituto Federal de Educação, Ciência e Tecnologia do Espírito Santo (IFES), Av. Ministro Salgado Filho, Soteco, Vila Velha, Espírito Santo 29106-010, BrazilMore by Wanderson Romão
Abstract
Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) can determine the chemical identity and spatial distribution of several molecules in a single analysis, conserving its natural histology. However, there are no specific studies on the spatial distribution of alkaloids in Erythroxylum coca leaves by MALDI IMS, preserving the histology of the monitored compounds. Therefore, in this work, positive-ion mode MALDI Fourier-transform ion cyclotron resonance imaging mass spectrometry (MALDI(+)FT–ICR IMS) was applied to identify and analyze the distribution of alkaloids on the surface of coca leaves, evaluating the ionization efficiency of three matrices (α-cyano-4-hydroxycinnamic acid (CHCA), 2-mercaptobenzothiazole (MBT), and 2,5-dihydroxybenzoic acid (DHB)). The last was chosen as the best matrix in this study, and it was studied in five concentrations (0.5, 1.0, 2.0, 4.0, and 8.0 mg·mL–1), where 2 mg·mL–1 was the most efficient. The washing of coca leaves with the organic solvents (acetonitrile, methanol, toluene, and dichloromethane) tested did not improve the performance of the ionization process. Finally, a tissue section, 50 μm thick, was used to study the inner part of the leaf tissue, where alkaloids and flavonoid molecules were detected.
Cited By
Smart citations by scite.ai include citation statements extracted from the full text of the citing article. The number of the statements may be higher than the number of citations provided by ACS Publications if one paper cites another multiple times or lower if scite has not yet processed some of the citing articles.
This article is cited by 18 publications.
- Ronald Marentes-Culma, Luisa Lorena Orduz-Díaz, Kimberly Lozano-Garzón, Marcela Piedad Carrillo. From Tradition to Science: Chemical, Nutritional, and Cytotoxic Characterization of Erythroxylum coca from Indigenous Colombian Communities. ACS Omega 2025, 10
(15)
, 15009-15017. https://doi.org/10.1021/acsomega.4c10491
- Jessica L. Moore, Georgia Charkoftaki. A Guide to MALDI Imaging Mass Spectrometry for Tissues. Journal of Proteome Research 2023, 22
(11)
, 3401-3417. https://doi.org/10.1021/acs.jproteome.3c00167
- Ana Claudia Hertel Pereira, Ana Carolina Auer, Lauro Biedel, Camila Medeiros de Almeida, Wanderson Romão, Denise Coutinho Endringer. Analysis of Gliricidia sepium Leaves by MALDI Mass Spectrometry Imaging. Journal of the American Society for Mass Spectrometry 2022, 33
(3)
, 573-583. https://doi.org/10.1021/jasms.1c00367
- Sevilay Erdogan-Kablan, Seyda Yayla, M. Mesud Hurkul, Ahmet Cetinkaya, Emirhan Nemutlu, Sibel A. Ozkan. Recent advancements in the bioactive alkaloids analysis in plant and biological specimen: From the perspective of activity, sample preparation, and analytical method selection. Journal of Chromatography B 2025, 1258 , 124592. https://doi.org/10.1016/j.jchromb.2025.124592
- Guadalupe García Romero, Víctor Fabian Ruíz Ruíz, Alien Blanco Flores, Helen Paola Toledo Jaldin, Alfredo Rafael Vilchis-Nestor, Delia M. Ávila-Márquez, Delfino Reyes Contreras. Light-driven synthesis of silver nanoparticles using Erythroxylum coca extract for catalytic reduction of 4-nitrophenol. Biocatalysis and Agricultural Biotechnology 2025, 64 , 103492. https://doi.org/10.1016/j.bcab.2025.103492
- Camila M. de Almeida, Nayara A. dos Santos, Valdemar Lacerda, Xin Ma, Facundo M. Fernández, Wanderson Romão. Applications of MALDI mass spectrometry in forensic science. Analytical and Bioanalytical Chemistry 2024, 416
(24)
, 5255-5280. https://doi.org/10.1007/s00216-024-05470-y
- Shelby D. Oney-Hawthorne, David P. Barondeau. Fe-S cluster biosynthesis and maturation: Mass spectrometry-based methods advancing the field. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 2024, 1871
(7)
, 119784. https://doi.org/10.1016/j.bbamcr.2024.119784
- Benjamin Gabriel Chavez, Sara Leite Dias, John Charles D'Auria. The evolution of tropane alkaloids: Coca does it differently. Current Opinion in Plant Biology 2024, 81 , 102606. https://doi.org/10.1016/j.pbi.2024.102606
- Qi Jing, Hongda Wang, Xue Li, Dianxin Cui, Yuying Zhao, Wei Wang, Ying Hu, Meiting Jiang, Xiumei Gao, Dean Guo, Wenzhi Yang. State-of-the-art application of mass spectrometry imaging covering the medicinal and edible plants. TrAC Trends in Analytical Chemistry 2024, 179 , 117878. https://doi.org/10.1016/j.trac.2024.117878
- Mudita Vats, Berta Cillero-Pastor, Eva Cuypers, Ron M. A. Heeren. Mass spectrometry imaging in plants, microbes, and food: a review. The Analyst 2024, 149
(18)
, 4553-4582. https://doi.org/10.1039/D4AN00644E
- Nancy Shyrley García-Rojas, Carlos Daniel Sierra-Álvarez, Hilda E. Ramos-Aboites, Abigail Moreno-Pedraza, Robert Winkler. Identification of Plant Compounds with Mass Spectrometry Imaging (MSI). Metabolites 2024, 14
(8)
, 419. https://doi.org/10.3390/metabo14080419
- Clebson de Jesus Macrino, Nayara A. dos Santos, Nathália dos S. Conceição, Clara S. D. Baptista, Alvaro Cunha Neto, Wanderson Romão. Isolation and characterization of reference standard candidates for cocaine and benzoylecgonine obtained from illicit substances seized. Journal of Forensic Sciences 2024, 69
(3)
, 1025-1034. https://doi.org/10.1111/1556-4029.15502
- Patrick J Horn, Kent D Chapman, . Imaging plant metabolism
in situ. Journal of Experimental Botany 2024, 75
(6)
, 1654-1670. https://doi.org/10.1093/jxb/erad423
- Marisa Maia, Andreia Figueiredo, Carlos Cordeiro, Marta Sousa Silva. FT‐ICR‐MS‐based metabolomics: A deep dive into plant metabolism. Mass Spectrometry Reviews 2023, 42
(5)
, 1535-1556. https://doi.org/10.1002/mas.21731
- Jiaman Lin, Keming Yun, Qiran Sun, Ping Xiang, Lina Wu, Shuo Yang, Junling Dun, Shanlin Fu, Hang Chen. How to sample a seizure plant: the role of the visualization spatial distribution analysis of
Lophophora williamsii
as an example. Forensic Sciences Research 2023, 8
(2)
, 140-151. https://doi.org/10.1093/fsr/owad014
- Valentina Calabrese, Isabelle Schmitz-Afonso, Wassila Riah-Anglet, Isabelle Trinsoutrot-Gattin, Barbara Pawlak, Carlos Afonso. Direct introduction MALDI FTICR MS based on dried droplet deposition applied to non-targeted metabolomics on Pisum Sativum root exudates. Talanta 2023, 253 , 123901. https://doi.org/10.1016/j.talanta.2022.123901
- David Love, Nicole S. Jones. Interpol Review of Drug Analysis 2019-2022. Forensic Science International: Synergy 2023, 6 , 100299. https://doi.org/10.1016/j.fsisyn.2022.100299
- Julio de Carvalho Ponce, Luiz Ferreira Neves Neves Junior, Ana Carolina Stosch da Silva, Luiz Carlos Liberatori, Priscila Vieira de Medeiros. Detection of cocaine crystals dispersed on non- Erythroxylum herbs. Forensic Science International 2022, 332 , 111209. https://doi.org/10.1016/j.forsciint.2022.111209
Article Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.
Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.
The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated.