Single Cell MALDI-MSI Analysis of Lipids and Proteins within a Replicative Senescence Fibroblast ModelClick to copy article linkArticle link copied!
- Emily R. SekeraEmily R. SekeraDepartment of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio 43210 United StatesMore by Emily R. Sekera
- Lorena RosasLorena RosasDepartment of Internal Medicine, Division of Pulmonary, Critical Care and Sleep Medicine, The Ohio State University, Columbus, Ohio 43210 United StatesMore by Lorena Rosas
- Joseph H. HolbrookJoseph H. HolbrookDepartment of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio 43210 United StatesMore by Joseph H. Holbrook
- Quetzalli D. Angeles-LopezQuetzalli D. Angeles-LopezDepartment of Internal Medicine, Division of Pulmonary, Critical Care and Sleep Medicine, The Ohio State University, Columbus, Ohio 43210 United StatesMore by Quetzalli D. Angeles-Lopez
- Timur KhaliullinTimur KhaliullinDepartment of Internal Medicine, Division of Pulmonary, Critical Care and Sleep Medicine, The Ohio State University, Columbus, Ohio 43210 United StatesMore by Timur Khaliullin
- Mauricio RojasMauricio RojasDepartment of Internal Medicine, Division of Pulmonary, Critical Care and Sleep Medicine, The Ohio State University, Columbus, Ohio 43210 United StatesMore by Mauricio Rojas
- Ana L. MoraAna L. MoraDepartment of Internal Medicine, Division of Pulmonary, Critical Care and Sleep Medicine, The Ohio State University, Columbus, Ohio 43210 United StatesMore by Ana L. Mora
- Amanda B. Hummon*Amanda B. Hummon*Email: [email protected]Department of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio 43210 United StatesMore by Amanda B. Hummon
Abstract

In this study, we evaluate lipids and select proteins in human lung fibroblasts (hLFs) to interrogate changes occurring due to aging and senescence. To study single cell populations, a comparison of cells adhered onto slides using poly-d-lysine versus centrifugal force deposition was first analyzed to determine whether specific alterations were observed between preparations. The poly-d-lysine approach was then utilized to interrogate the lipidome of the cell populations and further evaluate potential applications of the MALDI-immunohistochemistry (IHC) platform for single-cell-level analyses. Two protein markers of senescence, vimentin and p21, were both observed within the fibroblast populations and quantified. Lipidomic analysis of the fibroblasts found 12 lipids significantly altered because of replicative senescence, including fatty acids, such as stearic acid, and ceramide phosphoethanolamine species (CerPE). Similar to previous reports, alterations were detected in putative fatty acid building blocks, ceramides, among other lipid species. Altogether, our results reveal the ability to detect lipids implicated in senescence and show alterations to protein expression between normal and senescent fibroblast populations, including differences between young and aged cells. This report is the first time that the MALDI-IHC system has been utilized at a single-cell level to analyze both protein expression and lipid profiles in cultured cells, with a particular focus on changes associated with aging and senescence.
Cited By
This article has not yet been cited by other publications.
Article Views
Altmetric
Citations
Article Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.
Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.
The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated.