ACS Publications. Most Trusted. Most Cited. Most Read
My Activity
CONTENT TYPES

Figure 1Loading Img

Olfactory and Quantitative Analysis of Aroma Compounds in Elder Flower (Sambucus nigra L.) Drink Processed from Five Cultivars

View Author Information
Department of Fruit, Vegetable and Food Science, Danish Institute of Agricultural Sciences, Kirstinebjergvej 10, DK-5792 Aarslev, Denmark
Cite this: J. Agric. Food Chem. 2000, 48, 6, 2376–2383
Publication Date (Web):May 12, 2000
https://doi.org/10.1021/jf000005f
Copyright © 2000 American Chemical Society

    Article Views

    1300

    Altmetric

    -

    Citations

    LEARN ABOUT THESE METRICS
    Other access options

    Abstract

    Fresh elder flowers (Sambucus nigra L.) were extracted with an aqueous solution containing sucrose, peeled lemon slices, tartaric acid, and sodium benzoate to make elder flower syrup. Aroma compounds emitted from the elder flower syrup were collected by the dynamic headspace technique and analyzed by GC−FID and GC−MS. A total of 59 compounds were identified, 18 of which have not previously been detected in elder flower products. The concentrations of the identified volatiles were measured in five elder cultivars, Allesoe, Donau, Sambu, Sampo, and Samyl, and significant differences were detected among cultivars in the concentration levels of 48 compounds. The odor of the volatiles was evaluated by the GC-sniffing technique. cis-Rose oxide, nerol oxide, hotrienol, and nonanal contributed to the characteristic elder flower odor, whereas linalool, α-terpineol, 4-methyl-3-penten-2-one, and (Z)-β-ocimene contributed with floral notes. Fruity odors were associated with pentanal, heptanal, and β-damascenone. Fresh and grassy odors were primarily correlated with hexanal, hexanol, and (Z)-3-hexenol.

    Keywords: Sambucus nigra; Caprifoliaceae; volatiles; aroma compounds; dynamic headspace; olfactometry; odor classification

    Read this article

    To access this article, please review the available access options below.

    Get instant access

    Purchase Access

    Read this article for 48 hours. Check out below using your ACS ID or as a guest.

    Recommended

    Access through Your Institution

    You may have access to this article through your institution.

    Your institution does not have access to this content. You can change your affiliated institution below.

    *

     Author to whom correspondence should be addressed (telephone +45 6390 4343; fax +45 6390 4395; e-mail [email protected]).

    Cited By

    This article is cited by 64 publications.

    1. Katherine R. Uhl, Alyson E. Mitchell. Headspace Volatile Organic and Phenolic Compounds in Elderflowers and Elderflower Teas of Blue Elderberry (Sambucus nigra ssp. cerulea). ACS Food Science & Technology 2022, 2 (10) , 1535-1545. https://doi.org/10.1021/acsfoodscitech.2c00177
    2. Thirumurugan Rathinasabapathy, Lakshmana Prabu Sakthivel, Slavko Komarnytsky. Plant-Based Support of Respiratory Health during Viral Outbreaks. Journal of Agricultural and Food Chemistry 2022, 70 (7) , 2064-2076. https://doi.org/10.1021/acs.jafc.1c06227
    3. Maja Mikulic-Petkovsek, Justyna Samoticha, Klemen Eler, Franci Stampar, and Robert Veberic . Traditional Elderflower Beverages: A Rich Source of Phenolic Compounds with High Antioxidant Activity. Journal of Agricultural and Food Chemistry 2015, 63 (5) , 1477-1487. https://doi.org/10.1021/jf506005b
    4. Sumangala Bhattacharya, Kathrine B. Christensen, Louise C. B. Olsen, Lars P. Christensen, Kai Grevsen, Nils J. Færgeman, Karsten Kristiansen, Jette F. Young, and Niels Oksbjerg . Bioactive Components from Flowers of Sambucus nigra L. Increase Glucose Uptake in Primary Porcine Myotube Cultures and Reduce Fat Accumulation in Caenorhabditis elegans. Journal of Agricultural and Food Chemistry 2013, 61 (46) , 11033-11040. https://doi.org/10.1021/jf402838a
    5. Geoffrey C. Kite, Sonny Larsson, Nigel C. Veitch, Elaine A. Porter, Ning Ding, and Monique S. J. Simmonds . Acyl Spermidines in Inflorescence Extracts of Elder (Sambucus nigra L., Adoxaceae) and Elderflower Drinks. Journal of Agricultural and Food Chemistry 2013, 61 (14) , 3501-3508. https://doi.org/10.1021/jf304602q
    6. Berta Gonçalves, Virgílio Falco, José Moutinho-Pereira, Eunice Bacelar, Francisco Peixoto and Carlos Correia. Effects of Elevated CO2 on Grapevine (Vitis vinifera L.): Volatile Composition, Phenolic Content, and in Vitro Antioxidant Activity of Red Wine. Journal of Agricultural and Food Chemistry 2009, 57 (1) , 265-273. https://doi.org/10.1021/jf8020199
    7. Verónica Galindo-Cuspinera,, Meryl B. Lubran, and, Scott A. Rankin. Comparison of Volatile Compounds in Water- and Oil-Soluble Annatto (Bixa orellana L.) Extracts. Journal of Agricultural and Food Chemistry 2002, 50 (7) , 2010-2015. https://doi.org/10.1021/jf011325h
    8. Vidak Raičević, Marko Mladenović, Milica Aćimović, Niko Radulović. New esters from the essential oil of dry flowers of elder ( Sambucus nigra L.). Journal of the Science of Food and Agriculture 2024, 104 (3) , 1308-1321. https://doi.org/10.1002/jsfa.13012
    9. Paula Lizana, Ricardo Godoy, Francheska Martínez, Dieter Wicher, Sabine Kaltofen, Leonardo Guzmán, Oscar Ramírez, Diego Cifuentes, Ana Mutis, Herbert Venthur. A highly conserved plant volatile odorant receptor detects a sex pheromone component of the greater wax moth, Galleria mellonella (Lepidoptera: Pyralidae). Insect Biochemistry and Molecular Biology 2023, 163 , 104031. https://doi.org/10.1016/j.ibmb.2023.104031
    10. Anna Stój, Tomasz Czernecki, Dorota Domagała. Authentication of Polish Red Wines Produced from Zweigelt and Rondo Grape Varieties Based on Volatile Compounds Analysis in Combination with Machine Learning Algorithms: Hotrienol as a Marker of the Zweigelt Variety. Molecules 2023, 28 (4) , 1961. https://doi.org/10.3390/molecules28041961
    11. Eva Sánchez-Hernández, Joaquín Balduque-Gil, Vicente González-García, Juan J. Barriuso-Vargas, José Casanova-Gascón, Jesús Martín-Gil, Pablo Martín-Ramos. Phytochemical Profiling of Sambucus nigra L. Flower and Leaf Extracts and Their Antimicrobial Potential against Almond Tree Pathogens. International Journal of Molecular Sciences 2023, 24 (2) , 1154. https://doi.org/10.3390/ijms24021154
    12. Riccardo Motti, Bruno Paura, Alessia Cozzolino, Bruna de Falco. Edible Flowers Used in Some Countries of the Mediterranean Basin: An Ethnobotanical Overview. Plants 2022, 11 (23) , 3272. https://doi.org/10.3390/plants11233272
    13. Sandrine S. Ferreira, Amélia M. Silva, Fernando M. Nunes. Sambucus nigra L. Fruits and Flowers: Chemical Composition and Related Bioactivities. Food Reviews International 2022, 38 (6) , 1237-1265. https://doi.org/10.1080/87559129.2020.1788578
    14. Yi Bo Liu, Zhi Jiang Zeng, Andrew B. Barron, Ye Ma, Yu Zhu He, Jun Feng Liu, Zhen Li, Wei Yu Yan, Xu Jiang He. The involvement of a floral scent in plant-honeybee interaction. The Science of Nature 2022, 109 (3) https://doi.org/10.1007/s00114-022-01802-z
    15. Emmanuel Nyongesa Waswa, Jing Li, Elijah Mbandi Mkala, Vincent Okelo Wanga, Elizabeth Syowai Mutinda, Consolata Nanjala, Wyclif Ochieng Odago, Daniel Mutavi Katumo, Moses Kirega Gichua, Robert Wahiti Gituru, Guang-Wan Hu, Qing-Feng Wang. Ethnobotany, phytochemistry, pharmacology, and toxicology of the genus Sambucus L. (Viburnaceae). Journal of Ethnopharmacology 2022, 292 , 115102. https://doi.org/10.1016/j.jep.2022.115102
    16. Adriana Kolesarova, Simona Baldovska, Ladislav Kohut, Alexander V. Sirotkin. Black Elder and Its Constituents: Molecular Mechanisms of Action Associated with Female Reproduction. Pharmaceuticals 2022, 15 (2) , 239. https://doi.org/10.3390/ph15020239
    17. Anna Stój, Tomasz Czernecki, Bożena Sosnowska, Agnieszka Niemczynowicz, Arkadiusz Matwijczuk. Impact of Grape Variety, Yeast and Malolactic Fermentation on Volatile Compounds and Fourier Transform Infrared Spectra in Red Wines. Polish Journal of Food and Nutrition Sciences 2022, 2 , 38-55. https://doi.org/10.31883/pjfns/145665
    18. Adil Farooq Wali, Salma Jabnoun, Maryam Razmpoor, Fatima Najeeb, Hadeel Shalabi, Imra Akbar. Account of Some Important Edible Medicinal Plants and Their Socio-Economic Importance. 2022, 325-367. https://doi.org/10.1007/978-981-16-4880-9_14
    19. Alexander V. Sirotkin, Adriana Kolesarova. Food/medicinal herbs and their influence on health and female reproduction. 2022, 81-243. https://doi.org/10.1016/B978-0-12-824292-6.00003-9
    20. Basma Najar, Benedetta Ferri, Pier Luigi Cioni, Luisa Pistelli. Volatile emission and essential oil composition of Sambucus nigra L. organs during different developmental stages. Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology 2021, 155 (4) , 721-729. https://doi.org/10.1080/11263504.2020.1779841
    21. D.L. Capone, A. Barker, W. Pearson, I.L. Francis. Influence of inclusion of grapevine leaves, rachis and peduncles during fermentation on the flavour and volatile composition of Vitis vinifera cv. Shiraz wine. Australian Journal of Grape and Wine Research 2021, 27 (3) , 348-359. https://doi.org/10.1111/ajgw.12489
    22. Yini Yang, Qianchun Deng, Xiao Jia, Jie Shi, Chuyun Wan, Qi Zhou, Qiang Wang. Characterization of key odorants in peeled and unpeeled flaxseed powders using solvent-assisted flavor evaporation and odor activity value calculation. LWT 2021, 138 , 110724. https://doi.org/10.1016/j.lwt.2020.110724
    23. Mingwu Zang, Lan Wang, Zheqi Zhang, Kaihua Zhang, Dan Li, Xiaoman Li, Shouwei Wang, Hongzhang Chen. Changes in flavour compound profiles of precooked pork after reheating (warmed‐over flavour) using gas chromatography–olfactometry–mass spectrometry with chromatographic feature extraction. International Journal of Food Science & Technology 2020, 55 (3) , 978-987. https://doi.org/10.1111/ijfs.14306
    24. Javier Ruiz, Florian Kiene, Ignacio Belda, Daniela Fracassetti, Domingo Marquina, Eva Navascués, Fernando Calderón, Angel Benito, Doris Rauhut, Antonio Santos, Santiago Benito. Effects on varietal aromas during wine making: a review of the impact of varietal aromas on the flavor of wine. Applied Microbiology and Biotechnology 2019, 103 (18) , 7425-7450. https://doi.org/10.1007/s00253-019-10008-9
    25. Hale G. Ağalar. Elderberry (Sambucus nigra L.). 2019, 211-215. https://doi.org/10.1016/B978-0-12-812491-8.00030-8
    26. A. Morata, C. Vaquero, F. Palomero, I. Loira, M.A. Bañuelos, J.A. Suárez-Lepe. Technology of Vermouth Wines. 2019, 35-63. https://doi.org/10.1016/B978-0-12-815269-0.00002-7
    27. Annalisa Ricci, Alessia Levante, Martina Cirlini, Luca Calani, Valentina Bernini, Daniele Del Rio, Gianni Galaverna, Erasmo Neviani, Camilla Lazzi. The Influence of Viable Cells and Cell-Free Extracts of Lactobacillus casei on Volatile Compounds and Polyphenolic Profile of Elderberry Juice. Frontiers in Microbiology 2018, 9 https://doi.org/10.3389/fmicb.2018.02784
    28. Logan Strenchok, Panayiotis G. Dimitrakopoulos, Thanasis Kizos, Taxiarchoula-Maria Pitta. Local knowledge of selected wild plant species collected in Agiassos, on Lesvos, Greece. Norsk Geografisk Tidsskrift - Norwegian Journal of Geography 2018, 72 (5) , 273-286. https://doi.org/10.1080/00291951.2018.1497699
    29. Ângelo C. Salvador, Armando J. D. Silvestre, Sílvia M. Rocha. Comprehensive Insight into the Elderflowers and Elderberries (Sambucus nigra L.) Mono and Sesquiterpenic Metabolites: Factors that Modulate Their Composition. 2018https://doi.org/10.5772/intechopen.77291
    30. Annalisa Ricci, Martina Cirlini, Alessia Levante, Chiara Dall'Asta, Gianni Galaverna, Camilla Lazzi. Volatile profile of elderberry juice: Effect of lactic acid fermentation using L. plantarum , L. rhamnosus and L. casei strains. Food Research International 2018, 105 , 412-422. https://doi.org/10.1016/j.foodres.2017.11.042
    31. Poliana Cardoso-Gustavson, Silvia Ribeiro de Souza, Fábio de Barros. Floral volatile profile in Pleurothallidinae, an orchid subtribe pollinated by flies: ecological and phylogenetic considerations. Phytochemistry Letters 2017, 22 , 49-55. https://doi.org/10.1016/j.phytol.2017.09.005
    32. Tomáš Bajer, Petra Bajerová, Karel Ventura. Effect of Harvest and Drying on Composition of Volatile Profile of Elderflowers ( Sambucus nigra ) from Wild. Natural Product Communications 2017, 12 (12) , 1934578X1701201. https://doi.org/10.1177/1934578X1701201231
    33. Ângelo C. Salvador, Armando J.D. Silvestre, Sílvia M. Rocha. Unveiling elderflowers (Sambucus nigra L.) volatile terpenic and norisoprenoids profile: Effects of different postharvest conditions. Food Chemistry 2017, 229 , 276-285. https://doi.org/10.1016/j.foodchem.2017.02.037
    34. Magdalena Śliwińska, Paulina Wiśniewska, Tomasz Dymerski, Waldemar Wardencki, Jacek Namieśnik. Authenticity Assessment of the “Onisiówka” Nalewka Liqueurs Using Two-Dimensional Gas Chromatography and Sensory Evaluation. Food Analytical Methods 2017, 10 (6) , 1709-1720. https://doi.org/10.1007/s12161-016-0723-z
    35. Giang Thanh Thi Ho, Yuan-Feng Zou, Helle Wangensteen, Hilde Barsett. RG-I regions from elderflower pectins substituted on GalA are strong immunomodulators. International Journal of Biological Macromolecules 2016, 92 , 731-738. https://doi.org/10.1016/j.ijbiomac.2016.07.090
    36. Giang Thanh Thi Ho, Yuan-Feng Zou, Torun Helene Aslaksen, Helle Wangensteen, Hilde Barsett. Structural characterization of bioactive pectic polysaccharides from elderflowers ( Sambuci flos ). Carbohydrate Polymers 2016, 135 , 128-137. https://doi.org/10.1016/j.carbpol.2015.08.056
    37. Eva Vítová, Kateřina Sůkalová, Martina Mahdalová, Lenka Butorová, Libor Babák, Aleš Matějíček. Comparison of Flavour and Volatile Flavour Compounds of Mixed Elderberry Juices. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 2015, 63 (1) , 147-152. https://doi.org/10.11118/actaun201563010147
    38. Manuela Mandrone, Beatrice Lorenzi, Antonella Maggio, Tommaso La Mantia, Monica Scordino, Maurizio Bruno, Ferruccio Poli. Polyphenols pattern and correlation with antioxidant activities of berries extracts from four different populations of Sicilian Sambucus nigra L.. Natural Product Research 2014, 28 (16) , 1246-1253. https://doi.org/10.1080/14786419.2014.898147
    39. M.P. Mendez-Costabel, K.L. Wilkinson, S.E.P. Bastian, C. Jordans, M. McCarthy, C.M. Ford, N. Dokoozlian. Effect of winter rainfall on yield components and fruit green aromas of V itis vinifera  L. cv. Merlot in California. Australian Journal of Grape and Wine Research 2014, 20 (1) , 100-110. https://doi.org/10.1111/ajgw.12060
    40. M.P. Mendez-Costabel, K.L. Wilkinson, S.E.P. Bastian, C. Jordans, M. McCarthy, C.M. Ford, N.K. Dokoozlian. Effect of increased irrigation and additional nitrogen fertilisation on the concentration of green aroma compounds in V itis vinifera L. Merlot fruit and wine. Australian Journal of Grape and Wine Research 2014, 20 (1) , 80-90. https://doi.org/10.1111/ajgw.12062
    41. Nicole Pabi, Georg Innerhofer, Erich Leitner, Barbara Siegmund. The Flavor of Elderflower – Species Differentiation via Flavor Compounds. 2014, 95-99. https://doi.org/10.1016/B978-0-12-398549-1.00017-9
    42. Jie Lin, Yi Dai, Ya-nan Guo, Hai-rong Xu, Xiao-chang Wang. Volatile profile analysis and quality prediction of Longjing tea (Camellia sinensis) by HS-SPME/GC-MS. Journal of Zhejiang University SCIENCE B 2012, 13 (12) , 972-980. https://doi.org/10.1631/jzus.B1200086
    43. Jie Feng, Xiao‐Bei Zhan, Zhi‐Yong Zheng, Dong Wang, Li‐Min Zhang, Chi‐Chung Lin. A two‐step inoculation of Candida etchellsii to enhance soy sauce flavour and quality. International Journal of Food Science & Technology 2012, 47 (10) , 2072-2078. https://doi.org/10.1111/j.1365-2621.2012.03071.x
    44. G.B. Fanaro, R.C. Duarte, A.G. Santillo, M.E.M. Pinto e Silva, E. Purgatto, A.L.C.H. Villavicencio. Evaluation of γ-radiation on oolong tea odor volatiles. Radiation Physics and Chemistry 2012, 81 (8) , 1152-1156. https://doi.org/10.1016/j.radphyschem.2011.11.061
    45. T. K. Lim. Sambucus nigra. 2012, 30-44. https://doi.org/10.1007/978-90-481-8661-7_5
    46. E. Dadáková, N. Vrchotová, Š. Chmelová, B. Šerá. The stability of rutin and chlorogenic acid during the processing of black elder (Sambucus nigra) inflorescence. Acta Alimentaria 2011, 40 (3) , 327-334. https://doi.org/10.1556/AAlim.2010.0010
    47. G.B. Fanaro, R.C. Duarte, M.M. Araújo, E. Purgatto, A.L.C.H. Villavicencio. Evaluation of γ-radiation on green tea odor volatiles. Radiation Physics and Chemistry 2011, 80 (1) , 85-88. https://doi.org/10.1016/j.radphyschem.2010.08.008
    48. Denis Charlebois, Patrick L. Byers, Chad E. Finn, Andrew L. Thomas. Elderberry: Botany, Horticulture, Potential. 2010, 213-280. https://doi.org/10.1002/9780470543672.ch4
    49. Peter S. J. Cheetham. Natural Sources of Flavours. 2010, 127-177. https://doi.org/10.1002/9781444317770.ch5
    50. J.D. Dunlevy, C.M. Kalua, R.A. Keyzers, P.K. Boss. The Production of Flavour & Aroma Compounds in Grape Berries. 2009, 293-340. https://doi.org/10.1007/978-90-481-2305-6_11
    51. Nadia Sabatini, Maria Regina Mucciarella, Vincenzo Marsilio. Volatile compounds in uninoculated and inoculated table olives with Lactobacillus plantarum (Olea europaea L., cv. Moresca and Kalamata). LWT - Food Science and Technology 2008, 41 (10) , 2017-2022. https://doi.org/10.1016/j.lwt.2007.12.002
    52. Karl Kaack, Lars P. Christensen. Effect of packing materials and storage time on volatile compounds in tea processed from flowers of black elder (Sambucus nigra L.). European Food Research and Technology 2008, 227 (4) , 1259-1273. https://doi.org/10.1007/s00217-008-0844-8
    53. K. Kaack. Processing of aroma extracts from elder flower (Sambucus nigra L.). European Food Research and Technology 2008, 227 (2) , 375-390. https://doi.org/10.1007/s00217-007-0732-7
    54. K. Kaack. Aroma composition and sensory quality of fruit juices processed from cultivars of elderberry (Sambucus nigra L.). European Food Research and Technology 2008, 227 (1) , 45-56. https://doi.org/10.1007/s00217-007-0691-z
    55. Lars P. Christensen, Karl Kaack, Xavier C. Fretté. Selection of elderberry (Sambucus nigra L.) genotypes best suited for the preparation of elderflower extracts rich in flavonoids and phenolic acids. European Food Research and Technology 2008, 227 (1) , 293-305. https://doi.org/10.1007/s00217-007-0723-8
    56. R. Di Cagno, S. Buchin, S. de Candia, M. De Angelis, P.F. Fox, M. Gobbetti. Characterization of Italian Cheeses Ripened Under Nonconventional Conditions. Journal of Dairy Science 2007, 90 (6) , 2689-2704. https://doi.org/10.3168/jds.2006-654
    57. K. Kaack, L. P. Christensen, M. Hughes, R. Eder. Relationship between sensory quality and volatile compounds of elderflower (Sambucus nigra L.) extracts. European Food Research and Technology 2006, 223 (1) , 57-70. https://doi.org/10.1007/s00217-005-0122-y
    58. A. Wajs, A. Pranovich, M. Reunanen, S. Willför, B. Holmbom. Characterisation of volatile organic compounds in stemwood using solid-phase microextraction. Phytochemical Analysis 2006, 17 (2) , 91-101. https://doi.org/10.1002/pca.891
    59. K. Kaack, L. P. Christensen, M. Hughes, R. Eder. The relationship between sensory quality and volatile compounds in raw juice processed from elderberries (Sambucus nigra L.). European Food Research and Technology 2005, 221 (3-4) , 244-254. https://doi.org/10.1007/s00217-005-1141-4
    60. Michael C. Qian, Yuanyuan Wang. Seasonal Variation of Volatile Composition and Odor Activity Value of‘Marion’(Rubus spp. hyb) and‘Thornless Evergreen’(R. laciniatus L.) Blackberries. Journal of Food Science 2005, 70 (1) , C13-C20. https://doi.org/10.1111/j.1365-2621.2005.tb09013.x
    61. Mari R. Tellez, Ikhlas A. Khan, Brian T. Schaneberg, Sara L. Crockett, Agnes M. Rimando, Mozaina Kobaisy. Steam distillation–solid-phase microextraction for the detection of Ephedra sinica in herbal preparations. Journal of Chromatography A 2004, 1025 (1) , 51-56. https://doi.org/10.1016/S0021-9673(03)01035-5
    62. Mark D. Atkinson, Elaine Atkinson. Sambucus nigra L.. Journal of Ecology 2002, 90 (5) , 895-923. https://doi.org/10.1046/j.1365-2745.2002.00698.x
    63. Karina Jensen, Lars P Christensen, Merete Hansen, Ulla J�rgensen, Karl Kaack. Olfactory and quantitative analysis of volatiles in elderberry (Sambucus nigra L) juice processed from seven cultivars. Journal of the Science of Food and Agriculture 2001, 81 (2) , 237-244. https://doi.org/10.1002/1097-0010(20010115)81:2<237::AID-JSFA809>3.0.CO;2-H
    64. . Current Awareness. Flavour and Fragrance Journal 2000, 362-369. https://doi.org/10.1002/1099-1026(200009/10)15:5<362::AID-FFJ899>3.0.CO;2-A

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    You’ve supercharged your research process with ACS and Mendeley!

    STEP 1:
    Click to create an ACS ID

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    MENDELEY PAIRING EXPIRED
    Your Mendeley pairing has expired. Please reconnect