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Application of NMR Spectroscopy and LC-NMR/MS to the Identification of Carbohydrates in Beer

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    Application of NMR Spectroscopy and LC-NMR/MS to the Identification of Carbohydrates in Beer
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    Department of Chemistry, Campus de Santiago, University of Aveiro, 3810−193 Aveiro, Portugal and Bruker Biospin GmbH, Silberstreifen, D76287 Rheinstetten, Germany
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    Journal of Agricultural and Food Chemistry

    Cite this: J. Agric. Food Chem. 2003, 51, 17, 4847–4852
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    https://doi.org/10.1021/jf030097j
    Published July 10, 2003
    Copyright © 2003 American Chemical Society
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    Abstract

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    The application of LC-NMR/MS for the direct identification of carbohydrates in beer has been studied. Carbohydrates are major beer components, and their structural characterization by NMR alone is seriously hindered by strong spectroscopic overlap. Direct analysis of beer by LC-NMR/MS enables the rapid (1−2 h) identification of dextrins with degree of polymerization (DP) of up to nine monomers, with degassing being the only sample treatment required. Although the presence of α(1→6) branching points is easily indicated by NMR for each subfraction separated by LC, difficulties arise for the unambiguous assignment of linear or branched forms of high DP dextrins. The two beer samples investigated in this work were found to have significantly different oligosaccharide compositions, reflecting the different production conditions employed. The use of hyphenated NMR for the rapid characterization of the carbohydrate composition of beers may be the basis of a useful tool for the quality control of beer.

    Keywords: Beer; NMR; LC-NMR/MS; carbohydrate; dextrins; composition

    Copyright © 2003 American Chemical Society

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    †

     University of Aveiro.

    ‡

     Bruker Biospin GmbH.

    *

     To whom correspondence should be addressed. Tel.:  351 234 370707. Fax:  351 234 370084. E-mail:  [email protected].

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    Cite this: J. Agric. Food Chem. 2003, 51, 17, 4847–4852
    Click to copy citationCitation copied!
    https://doi.org/10.1021/jf030097j
    Published July 10, 2003
    Copyright © 2003 American Chemical Society
    Request reuse permissions

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