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New Enzymatic Method for the Determination of Total Phenolic Content in Tea and Wine
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    New Enzymatic Method for the Determination of Total Phenolic Content in Tea and Wine
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    Department of Physical Chemistry, University of Venice, Dorsoduro 2137, 30123 Venice, Italy
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    Journal of Agricultural and Food Chemistry

    Cite this: J. Agric. Food Chem. 2004, 52, 20, 6287–6293
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    https://doi.org/10.1021/jf049898s
    Published September 14, 2004
    Copyright © 2004 American Chemical Society

    Abstract

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    A new spectrophotometric enzymatic method for the determination of total phenol content in tea and wine has been developed. The method is based on the peroxidase-catalyzed oxidation, by hydrogen peroxide, of phenols to phenoxyl radicals, which can react with aromatic substrates to form intensely colored adducts. In comparison with the widely used Folin−Ciocalteu method, this method appears to be more specific and more rapid and as a whole is not affected by the common interfering substances such as ascorbate, citrate, and sulfite. Numerous samples of teas and wines were analyzed by using the new method, and the results compared with those obtained by using the Folin and scavenging of DPPH methods. The differences of the total phenols content found by applying the three methods are discussed in terms of the different specificities of the analytical basis.

    Keywords: Total polyphenols; enzymatic analysis; tea; wine

    Copyright © 2004 American Chemical Society

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    Journal of Agricultural and Food Chemistry

    Cite this: J. Agric. Food Chem. 2004, 52, 20, 6287–6293
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    https://doi.org/10.1021/jf049898s
    Published September 14, 2004
    Copyright © 2004 American Chemical Society

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