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Selenium Species in Aqueous Extracts of Alfalfa Sprouts by Two-Dimensional Liquid Chromatography Coupled to Inductively Coupled Plasma Mass Spectrometry and Electrospray Mass Spectrometry Detection

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Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, C/Julián Clavería 8, 33006 Oviedo, Spain, and Department of Applied Chemistry, Faculty of Food Science, Corvinus University of Budapest, H-1118 Budapest, Villányi út 29-33, Hungary
Cite this: J. Agric. Food Chem. 2006, 54, 13, 4524–4530
Publication Date (Web):June 2, 2006
https://doi.org/10.1021/jf060320n
Copyright © 2006 American Chemical Society
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    Abstract

    The complementary use of two different liquid chromatographic mechanisms coupled to inductively coupled plasma mass spectrometry (ICP-MS) for selenium (Se) specific detection has permitted the screening of the most abundant Se-containing fractions in selenized alfalfa sprouts (Medicago sativa). Aqueous extracts of the sprouts were fractionated first by size exclusion chromatography (SEC) using a Superdex Peptide column and a mobile phase containing an ammonium acetate buffer (pH 7). Further purification of the individual SEC Se-containing fractions was carried out using two different chromatographic systems:  a Shodex Ashaipack column, with a mixed mechanism of size exclusion and ion exchange, and a conventional reversed phase C8 using ion-pairing reagents. In both cases, the columns were coupled to an inductively coupled plasma mass spectrometer equipped with an octapole reaction system for Se specific detection. This system allowed the on-line monitoring of the most abundant Se isotopes (78Se, 80Se) by reducing the possible polytomic interferences affecting these ions by adding hydrogen (2 mL min-1) to the octapole reaction cell. The results obtained by both separation mechanisms were highly comparable, revealing the presence of Se-methionine and Se-methyl selenocysteine. Both compounds were then confirmed by analyzing the corresponding fractions by electrospray quadrupole-time-of-flight (ESI-Q-TOF) mass spectrometry. Finally, an additional Se-containing species showing Se isotope distribution was detected at a molecular ion m/z 239 in the ESI-Q-TOF. The collision-induced dissociation of the m/z 239 and 237 ions (corresponding to 80Se and 78Se isotopes, respectively) revealed the possible presence as well of a derivative of the Se-2-propenyl selenocysteine.

    Keywords: Selenium; alfalfa sprouts; HPLC-ICP-MS; ESI-Q-TOF

    †

     University of Oviedo.

    ‡

     Corvinus University of Budapest.

    *

     To whom correspondence should be addressed.

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