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Effect of Postharvest Storage on the Expression of the Apple Allergen Mal d 1

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Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, United Kingdom, Norfolk Fruit Growers Ltd., Tunstead Road, Hoveton, United Kingdom, Plant Biotechnology Unit, IAM, BOKU, Vienna, Austria, Laboratory of Allergy Research, Department of Experimental Immunology, Amsterdam Medical Centre, Amsterdam, The Netherlands, and Department of Pathophysiology, Medical University of Vienna, Waehringer Guertel, Vienna, Austria
Cite this: J. Agric. Food Chem. 2006, 54, 16, 5917–5923
Publication Date (Web):July 19, 2006
https://doi.org/10.1021/jf060880m
Copyright © 2006 American Chemical Society

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    Abstract

    Consumption of fresh apples can cause allergy in susceptible individuals. A competitive enzyme-linked immunosorbent assay (ELISA) has been developed to determine Mal d 1 levels in apple pulp using a monoclonal antibody (BIP-1). The ELISA was able to rank ten cultivars according to their Mal d 1 content (between 3.8 and 72.5 μg/g pulp). For the first time, it has been demonstrated that growing conditions and postharvest storage, using three different treatments over a 5 month period in 2 consecutive years, increase Mal d 1 expression at a translational and transcriptional level (3.5- and 8.5-fold under controlled atmosphere storage). Expression of three major Mal d 1 isoforms was observed by real-time polymerase chain reaction over the 5 month storage period, and Mal d 1.02 was the most highly expressed isoform. In conclusion, Mal d 1 gene expression was significantly increased during modified atmosphere storage. Individuals suffering from birch pollen−apple allergy syndrome might experience fewer problems consuming freshly picked apples.

    Keywords: Apple; allergens; Bet v 1; cultivar; Mal d 1; Mal d 1 expression; PR 10; storage

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    *

     To whom correspondence should be addressed. Tel:  +44 1603 255000. Fax:  +44 1603 507723. E-mail:  [email protected].

     Institute of Food Research.

     Norfolk Fruit Growers Ltd..

    §

     BOKU.

     Amsterdam Medical Centre.

     Medical University of Vienna.

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