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Isolation and Identification of the DNA Aptamer Target to Acetamiprid

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College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, People’s Republic of China
Institute of Food Quality Safety and Detection, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Food Quality and Safety of Jiangsu Province, Key Laboratory of Food Safety Monitoring and Management, Ministry of Agriculture, Nanjing, Jiangsu 210014, People’s Republic of China
*Telephone: +86-25-8439 0401. Fax: +86-25-8439 0401. E-mail: [email protected]
Cite this: J. Agric. Food Chem. 2011, 59, 5, 1582–1586
Publication Date (Web):February 9, 2011
https://doi.org/10.1021/jf104189g
Copyright © 2011 American Chemical Society
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Abstract

As an alternative to antibodies, aptamers have a great potential as analytical tools for pesticide detection. In this work, aptamers targeting acetamiprid were selected by a specific systematic evolution of ligands by exponential enrichment (SELEX) strategy, where a single-stranded DNA (ssDNA) library was immobilized and target modification was eliminated. After 18 rounds of repeated selection, the ssDNA pool was enriched and then 14 sequences were selected and carefully identified. At last, an acetamiprid-specific aptamer with the apparent dissociation constant (Kd) estimated to be 4.98 μM was successfully obtained. Further work is ongoing to develop an aptamer-based detection method for field determination of this pesticides in agricultural products and environmental samples.

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