Oligosaccharide and Peptidoglycan of Ganoderma lucidum Activate the Immune Response in Human Mononuclear CellsClick to copy article linkArticle link copied!
- Chia-Che Tsai
- Feng-Ling Yang
- Zih-You Huang
- Chien-Sheng Chen
- Yu-Liang Yang
- Kuo-Feng Hua
- Jianjun Li
- Shui-Tein Chen
- Shih-Hsiung Wu
Abstract
The acid-hydrolyzed fragments of Ganoderma lucidum polysaccharides (GLPS) obtained by Smith degradation were separated by size-exclusion chromatography into two major water-soluble fractions: peptidoglycans (GLPS-SF1) and oligosaccharides (GLPS-SF2). Both fractions induced CD69 in human peripheral blood mononuclear cells (hPB-MNCs), and they displayed distinct immunomodulating properties. GLPS-SF1, with a molecular weight of around 20 kDa, were heterogeneous peptidoglycans composed of glucose/mannose (4:1) that exhibited biological activities with Th1 cytokines IL-12, IL-2, TNF-α, and IFN-γ in hPB-MNCs and stimulated macrophage cytokine expression via Toll-like receptor 4 (TLR4) signaling. For GLPS-SF2, with a molecular weight of around several kilodaltons, its sugar sequence was elucidated by mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy as [−α-1,4-Glc–(β-1,4-GlcA)3−]n. This oligosaccharide displayed specific immune property with low monocyte induction, greatly stimulated cell activation and proliferation of NK and T cells. This oligosaccharide isolated from G. lucidum polysaccharides with internal glucuronic acids/glucose repeat unit in a 3:1 ratio may be responsible for the active stimulation of NK and T cells.
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(18)
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(14)
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(4)
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- Lia Noemi Gerschenson, Ana Maria Rojas, Eliana Noemi Fissore. Carbohydrates. 2022, 49-126. https://doi.org/10.1016/B978-0-323-85052-0.00001-5
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(12)
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(16)
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(14)
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(7)
, 1797. https://doi.org/10.3390/molecules23071797
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(1)
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(2)
, 267-279. https://doi.org/10.1080/01635581.2016.1134599
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- Huabin Zhou, Gaoqiang Liu, Furu Huang, Xiangting Wu, Hailong Yang. Improved production, purification and bioactivity of a polysaccharide from submerged cultured Ganoderma lucidum. Archives of Pharmacal Research 2014, 37
(12)
, 1530-1537. https://doi.org/10.1007/s12272-014-0391-8
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