Lentils (Lens culinaris L.), a Rich Source of FolatesClick to copy article linkArticle link copied!
- Debjyoti Sen Gupta
- Dil Thavarajah
- Phil Knutson
- Pushparajah Thavarajah
- Rebecca J. McGee
- Clarice J. Coyne
- Shiv Kumar
Abstract

The potential for genetic biofortification of U.S.-grown lentils (Lens culinaris L.) with bioavailable folate has not been widely studied. The objectives of this study were (1) to determine the folate concentration of 10 commercial lentil cultivars grown in Minot and McLean counties, North Dakota, USA, in 2010 and 2011, (2) to determine the genotype (G) × environmental (E) interactions for folate concentration in lentil cultivars, and (3) to compare the folate concentration of other pulses [field peas (Pisum sativum L.) and chickpea (Cicer arietinum L.)] grown in the United States. Folate concentration in lentil cultivars ranged from 216 to 290 μg/100 g with a mean of 255 μg/100 g. In addition, lentil showed higher folate concentration compared to chickpea (42–125 μg/100 g), yellow field pea (41–55 μg/100 g), and green field pea (50–202 μg/100 g). A 100 g serving of lentils could provide a significant amount of the recommended daily allowance of dietary folates (54–73%) for adults. A significant year × location interaction on lentil folate concentration was observed; this indicates that possible location sourcing may be required for future lentil folate research.
Cited By
Smart citations by scite.ai include citation statements extracted from the full text of the citing article. The number of the statements may be higher than the number of citations provided by ACS Publications if one paper cites another multiple times or lower if scite has not yet processed some of the citing articles.
This article is cited by 59 publications.
- Jitendra Kumar, Debjyoti Sen Gupta, Shiv Kumar, Sanjeev Gupta, and Narendra Pratap Singh . Current Knowledge on Genetic Biofortification in Lentil. Journal of Agricultural and Food Chemistry 2016, 64
(33)
, 6383-6396. https://doi.org/10.1021/acs.jafc.6b02171
- Tomáš Siatka, Marek Mát’uš, Monika Moravcová, Patrícia Harčárová, Zuzana Lomozová, Kateřina Matoušová, Chaweewan Suwanvecho, Lenka Kujovská Krčmová, Přemysl Mladěnka. Biological, dietetic and pharmacological properties of vitamin B9. npj Science of Food 2025, 9
(1)
https://doi.org/10.1038/s41538-025-00396-w
- Mario González, Eleonora Barilli, Nicolas Rispail, Diego Rubiales. Optimization of inoculum production of Stemphylium botryosum for large-scale resistance screening of lentils. Plant Methods 2024, 20
(1)
https://doi.org/10.1186/s13007-024-01177-4
- Mark Dempsey, Dil Thavarajah. Low molecular weight carbohydrates and abiotic stress tolerance in lentil (Lens culinaris Medikus): a review. Frontiers in Plant Science 2024, 15 https://doi.org/10.3389/fpls.2024.1408252
- Uday Chand Jha, Harsh Nayyar, Mahender Thudi, Radha Beena, P. V. Vara Prasad, Kadambot H. M. Siddique. Unlocking the nutritional potential of chickpea: strategies for biofortification and enhanced multinutrient quality. Frontiers in Plant Science 2024, 15 https://doi.org/10.3389/fpls.2024.1391496
- Namrata Sanjeevi, Pablo Monsivais. Consumption Trends and Eating Context of Lentils and Dried Peas in the United States: A Nationally Representative Study. Nutrients 2024, 16
(2)
, 277. https://doi.org/10.3390/nu16020277
- Jitendra Kumar, Reyazul Rouf Mir, Asik Dutta, Archana Singh, Vinod Kumar, Sandhya Tyagi, Safoora Shafi, G.P. Dixit, Debjyoti Sen Gupta. Estimation of iron, zinc, phytic acid concentration and protein content in lentil seeds over locations and their marker-trait association analysis. Journal of Food Composition and Analysis 2024, 174 , 105999. https://doi.org/10.1016/j.jfca.2024.105999
- Gyan Prakash Mishra, Harsh Kumar Dikshit, Muraleedhar S. Aski, Akanksha Singh, Kuldeep Tripathi, Sanjeev Gupta, Shiv Kumar. Recent advancements in trait mapping in lentil (Lens culinaris Medik.). 2024, 93-130. https://doi.org/10.1016/B978-0-443-19409-2.00007-7
- Kuldeep Tripathi, Dorin Gupta, Padmavati G. Gore, Ravi Gowthami, Gyan Prakash Mishra, RK Pamarthi, Gayacharan, Harsh Kumar Dikshit, Shiv Kumar. Exploitation of lentil wild relatives for genetic improvement. 2024, 159-194. https://doi.org/10.1016/B978-0-443-19409-2.00013-2
- Suruchi Vij, Parul Sharma, Sonia Salaria, Nida Yousuf, Ashutosh Kushwah, Kuldeep Tripathi, Sarvjeet Singh. Lentil breeding. 2024, 43-92. https://doi.org/10.1016/B978-0-443-19409-2.00009-0
- Monika Jha, Rinky Resma Panda, Pooja Verma, K. R. Ramya, Padmavati G. Gore, Kuldeep Tripathi. Genetic Diversity Assessment in Grain Legumes. 2024, 399-426. https://doi.org/10.1007/978-981-99-5245-8_12
- Jeremy Marshall, Ana Vargas, Kirstin Bett. B vitamin quantification in lentil seed tissues using ultra-performance liquid chromatography-selected reaction monitoring mass spectrometry. Food Chemistry 2024, 430 , 136922. https://doi.org/10.1016/j.foodchem.2023.136922
- Nabila Begum, Qudrat Ullah Khan, Leyna G. Liu, Wenwen Li, Dahai Liu, Ijaz Ul Haq. Nutritional composition, health benefits and bio-active compounds of chickpea (Cicer arietinum L.). Frontiers in Nutrition 2023, 10 https://doi.org/10.3389/fnut.2023.1218468
- Fatema H. Brishti, Tareq Mzek, Nazamid Saari, Elenjikkal J. Rifna, Madhuresh Dwivedi, Kulwinder Kaur, Preetinder Kaur. Lentil Protein. 2023, 203-235. https://doi.org/10.1002/9781119866923.ch9
- Muraleedhar S. Aski, Gyan Prakash Mishra, Jayanti P. Tokkas, Prachi S. Yadav, Neha Rai, Ruchi Bansal, Akanksha Singh, Sanjeev Gupta, Jitendra Kumar, Ashok Parihar, Shiv Kumar, Vinod Kumar, Ashok Kumar Saxsena, Tapas Ranjan Das, Anil Kumar, Harsh Kumar Dikshit. Strategies for identifying stable lentil cultivars (Lens culinaris Medik) for combating hidden hunger, malnourishment, and climate variability. Frontiers in Plant Science 2023, 14 https://doi.org/10.3389/fpls.2023.1102879
- Semanur YILDIZ, Eylem KARAKUŞ, Serpil ÖZTÜRK. D-OPTIMAL MIXTURE DESIGN APPROACH IN THE PRODUCTION OF COOKIES ENRICHED WITH DIETARY FIBER SOURCES SUCH AS LENTIL FLOUR, BANANA FRUIT AND BANANA PEEL POWDER. Gıda 2023, 48
(1)
, 94-106. https://doi.org/10.15237/gida.GD22096
- Martina Foschi, Valerio Di Maria, Angelo Antonio D’Archivio, Federico Marini, Alessandra Biancolillo. E-Eye-Based Approach for Traceability and Annuality Compliance of Lentils. Applied Sciences 2023, 13
(3)
, 1433. https://doi.org/10.3390/app13031433
- Anirban Roy, Parmeshwar K. Sahu, Camellia Das, Somnath Bhattacharyya, Aamir Raina, Suvendu Mondal. Conventional and new-breeding technologies for improving disease resistance in lentil (Lens culinaris Medik). Frontiers in Plant Science 2023, 13 https://doi.org/10.3389/fpls.2022.1001682
- Debjyoti Sen Gupta, Jitendra Kumar, Surendra Barpate, A. K. Parihar, Anup Chandra, Anirban Roy, Ivica Djalovic. Lentils (Lens culinaris Medik): Nutritional Profile and Biofortification Prospects. 2023, 1-27. https://doi.org/10.1007/978-981-19-3627-2_27-1
- Aisha Umar, Zubaida Yousaf, Afifa Younas, Nadia Riaz, Madiha Rashid, Arusa Aftab, Zoya Kashif. Nutritional Security Approaches for Legume Biofortification—A Major Challenge. 2023, 17-45. https://doi.org/10.1007/978-3-031-33957-8_2
- Debjyoti Sen Gupta, Jitendra Kumar, Surendra Barpate, A. K. Parihar, Anup Chandra, Anirban Roy, Ivica Djalovic. Lentils (Lens culinaris Medik): Nutritional Profile and Biofortification Prospects. 2023, 719-745. https://doi.org/10.1007/978-981-19-4169-6_27
- Rintu Jha, Hemant Kumar Yadav, Rahul Raiya, Rajesh Kumar Singh, Uday Chand Jha, Lekshmy Sathee, Prashant Singh, Mahendar Thudi, Anshuman Singh, Sushil Kumar Chaturvedi, Shailesh Tripathi. Integrated breeding approaches to enhance the nutritional quality of food legumes. Frontiers in Plant Science 2022, 13 https://doi.org/10.3389/fpls.2022.984700
- Sapna Langyan, Pranjal Yadava, Fatima Nazish Khan, Ragini Bhardwaj, Kuldeep Tripathi, Vikash Bhardwaj, Rakesh Bhardwaj, Raj Kumar Gautam, Ashok Kumar. Nutritional and Food Composition Survey of Major Pulses Toward Healthy, Sustainable, and Biofortified Diets. Frontiers in Sustainable Food Systems 2022, 6 https://doi.org/10.3389/fsufs.2022.878269
- Lívia Hajas, László Sipos, Éva Csajbókné Csobod, Márta Veresné Bálint, Réka Juhász, Csilla Benedek. Lentil (Lens culinaris Medik.) Flour Varieties as Promising New Ingredients for Gluten-Free Cookies. Foods 2022, 11
(14)
, 2028. https://doi.org/10.3390/foods11142028
- Hasnae Choukri, Noureddine El Haddad, Khawla Aloui, Kamal Hejjaoui, Adil El-Baouchi, Abdelaziz Smouni, Dil Thavarajah, Fouad Maalouf, Shiv Kumar. Effect of High Temperature Stress During the Reproductive Stage on Grain Yield and Nutritional Quality of Lentil (Lens culinaris Medikus). Frontiers in Nutrition 2022, 9 https://doi.org/10.3389/fnut.2022.857469
- Sonia Salaria, Jon Lucas Boatwright, Pushparajah Thavarajah, Shiv Kumar, Dil Thavarajah. Protein Biofortification in Lentils (Lens culinaris Medik.) Toward Human Health. Frontiers in Plant Science 2022, 13 https://doi.org/10.3389/fpls.2022.869713
- Harsh Kumar Dikshit, Gyan P. Mishra, M. Aski, Akanksha Singh, P. S. Virk, Shiv Kumar. Lentil Biofortification. 2022, 271-293. https://doi.org/10.1007/978-981-16-3280-8_11
- Harsh Kumar Dikshit, Gyan Prakash Mishra, Muraleedhar S. Aski, Akanksha Singh, Kuldeep Tripathi, Ruchi Bansal, Aditya Pratap, Sanjeev Gupta, Shiv Kumar. Lentil Breeding. 2022, 1181-1236. https://doi.org/10.1007/978-981-16-9257-4_24
- A. K. Parihar, Rajesh Yadav, Amrit Lamichaney, R. K. Mishra, Anup Chandra, D. S. Gupta, Kuldeep Tripathi, K. K. Hazra, G. P. Dixit. Field Pea Breeding. 2022, 1237-1321. https://doi.org/10.1007/978-981-16-9257-4_25
- Lili Song, Diansi Yu, Hongjian Zheng, Guogan Wu, Yu Sun, Peng Li, Jinbin Wang, Cui Wang, Beibei Lv, Xueming Tang. Weighted gene co-expression network analysis unveils gene networks regulating folate biosynthesis in maize endosperm. 3 Biotech 2021, 11
(10)
https://doi.org/10.1007/s13205-021-02974-7
- Soma Gupta, Shouvik Das, Harsh Kumar Dikshit, Gyan Prakash Mishra, Muraleedhar S. Aski, Ruchi Bansal, Kuldeep Tripathi, Arpan Bhowmik, Shiv Kumar. Genotype by Environment Interaction Effect on Grain Iron and Zinc Concentration of Indian and Mediterranean Lentil Genotypes. Agronomy 2021, 11
(9)
, 1761. https://doi.org/10.3390/agronomy11091761
- Faruk Toklu, Debjyoti Sen Gupta, Tolga Karaköy, Hakan Özkan. Bioactives and Nutraceuticals in Food Legumes: Nutritional Perspective. 2021, 229-245. https://doi.org/10.1007/978-3-030-59215-8_10
- Anamika Tripathi, V. Iswarya, Narpinder Singh, Ashish Rawson. Chemistry of pulses—micronutrients. 2021, 61-86. https://doi.org/10.1016/B978-0-12-818184-3.00004-0
- Jitendra Kumar, Debjyoti Sen Gupta, Shiv Kumar. Genetic Potential of Lentil as a Nutritionally Rich Food Legume Crop. 2021, 83-98. https://doi.org/10.1007/978-3-030-59215-8_4
- A. K. Parihar, G. P. Dixit, U. Singh, Anil K. Singh, Nitin Kumar, Sanjeev Gupta. Potential of Field Pea as a Nutritionally Rich Food Legume Crop. 2021, 47-82. https://doi.org/10.1007/978-3-030-59215-8_3
- Martina Foschi, Angelo Antonio D'Archivio, Leucio Rossi. Geographical discrimination and authentication of lentils (Lens culinaris Medik.) by ICP-OES elemental analysis and chemometrics. Food Control 2020, 118 , 107438. https://doi.org/10.1016/j.foodcont.2020.107438
- Maša Vidmar Golja, Alenka Šmid, Nataša Karas Kuželički, Jurij Trontelj, Ksenija Geršak, Irena Mlinarič-Raščan. Folate Insufficiency Due to MTHFR Deficiency Is Bypassed by 5-Methyltetrahydrofolate. Journal of Clinical Medicine 2020, 9
(9)
, 2836. https://doi.org/10.3390/jcm9092836
- Ambuj B. Jha, Krishna K. Gali, Haixia Zhang, Randy W. Purves, Bunyamin Tar’an, Albert Vandenberg, Thomas D. Warkentin. Folate profile diversity and associated SNPs using genome wide association study in pea. Euphytica 2020, 216
(2)
https://doi.org/10.1007/s10681-020-2553-8
- Ambuj B. Jha, Thomas D. Warkentin. Biofortification of Pulse Crops: Status and Future Perspectives. Plants 2020, 9
(1)
, 73. https://doi.org/10.3390/plants9010073
- Arpita Das, Subrata Dutta, Subhendu Jash, Ashis Roy Barman, Raju Das, Shiv Kumar, Sanjeev Gupta. Current Knowledge on Pathogenicity and Management of Stemphylium botryosum in Lentils (Lens culinaris ssp. culinaris Medik). Pathogens 2019, 8
(4)
, 225. https://doi.org/10.3390/pathogens8040225
- Teresa Warne, Selena Ahmed, Carmen Byker Shanks, Perry Miller. Sustainability Dimensions of a North American Lentil System in a Changing World. Frontiers in Sustainable Food Systems 2019, 3 https://doi.org/10.3389/fsufs.2019.00088
- Haixia Zhang, Ambuj B. Jha, Devini De Silva, Randy W. Purves, Thomas D. Warkentin, Albert Vandenberg. Improved folate monoglutamate extraction and application to folate quantification from wild lentil seeds by ultra-performance liquid chromatography-selective reaction monitoring mass spectrometry. Journal of Chromatography B 2019, 1121 , 39-47. https://doi.org/10.1016/j.jchromb.2019.05.007
- Niroshan Siva, Pushparajah Thavarajah, Shiv Kumar, Dil Thavarajah. Variability in Prebiotic Carbohydrates in Different Market Classes of Chickpea, Common Bean, and Lentil Collected From the American Local Market. Frontiers in Nutrition 2019, 6 https://doi.org/10.3389/fnut.2019.00038
- M. Vidmar, J. Grželj, I. Mlinarič-Raščan, K. Geršak, M. Sollner Dolenc. Medicines associated with folate–homocysteine–methionine pathway disruption. Archives of Toxicology 2019, 93
(2)
, 227-251. https://doi.org/10.1007/s00204-018-2364-z
- Dil Thavarajah, Meredith McSwain, Casey R. Johnson, Shiv Kumar, Pushparajah Thavarajah. Pulses, Global Health, and Sustainability: Future Trends. 2019, 1-17. https://doi.org/10.1007/978-3-030-12763-3_1
- Adriana Paucean, Ovidiu P. Moldovan, Vlad Mureșan, Sonia A. Socaci, Francisc V. Dulf, Ersilia Alexa, Simona M. Man, Andruţa E. Mureșan, Sevastița Muste. Folic acid, minerals, amino-acids, fatty acids and volatile compounds of green and red lentils. Folic acid content optimization in wheat-lentils composite flours. Chemistry Central Journal 2018, 12
(1)
https://doi.org/10.1186/s13065-018-0456-8
- Jitendra Kumar, Dil Thavarajah, Shiv Kumar, Ashutosh Sarker, Narendra Pratap Singh. Analysis of genetic variability and genotype × environment interactions for iron and zinc content among diverse genotypes of lentil. Journal of Food Science and Technology 2018, 55
(9)
, 3592-3605. https://doi.org/10.1007/s13197-018-3285-9
- Haixia Zhang, Ambuj B. Jha, Thomas D. Warkentin, Albert Vandenberg, Randy W. Purves. Folate stability and method optimization for folate extraction from seeds of pulse crops using LC-SRM MS. Journal of Food Composition and Analysis 2018, 71 , 44-55. https://doi.org/10.1016/j.jfca.2018.04.008
- Niroshan Siva, Pushparajah Thavarajah, Dil Thavarajah. The impact of processing and cooking on prebiotic carbohydrates in lentil. Journal of Food Composition and Analysis 2018, 70 , 72-77. https://doi.org/10.1016/j.jfca.2018.04.006
- Dil Thavarajah, Alex Abare, Indika Mapa, Clarice Coyne, Pushparajah Thavarajah, Shiv Kumar. Selecting Lentil Accessions for Global Selenium Biofortification. Plants 2017, 6
(4)
, 34. https://doi.org/10.3390/plants6030034
- Niroshan Siva, Dil Thavarajah, Casey R. Johnson, Susan Duckett, Elliot D. Jesch, Pushparajah Thavarajah. Can lentil ( Lens culinaris Medikus) reduce the risk of obesity?. Journal of Functional Foods 2017, 38 , 706-715. https://doi.org/10.1016/j.jff.2017.02.017
- Aynur Gunenc, Melanie Ha Yeung, Christopher Lavergne, Jesse Bertinato, Farah Hosseinian. Enhancements of antioxidant activity and mineral solubility of germinated wrinkled lentils during fermentation in kefir. Journal of Functional Foods 2017, 32 , 72-79. https://doi.org/10.1016/j.jff.2017.02.016
- Clifford Hall, Cassandra Hillen, Julie Garden Robinson. Composition, Nutritional Value, and Health Benefits of Pulses. Cereal Chemistry 2017, 94
(1)
, 11-31. https://doi.org/10.1094/CCHEM-03-16-0069-FI
- Ramesh Kumar Saini, Shivraj Hariram Nile, Young-Soo Keum. Folates: Chemistry, analysis, occurrence, biofortification and bioavailability. Food Research International 2016, 89 , 1-13. https://doi.org/10.1016/j.foodres.2016.07.013
- Jagdish Singh, R.P. Srivastava, Shweta Gupta, P.S. Basu, Jitendra Kumar. Genetic Variability for Vitamin B9 and Total Dietary Fiber in Lentil (
Lens culinaris
L.) Cultivars. International Journal of Food Properties 2016, 19
(4)
, 936-943. https://doi.org/10.1080/10942912.2015.1048353
- Jitendra Kumar, Rohit Kant, Syed Mohd Quatadah, Shiv Kumar, Ashutosh Sarker. Biofortification Through Breeding Interventions in Lentil. 2016, 147-156. https://doi.org/10.1007/978-81-322-2716-8_12
- Kaliyaperumal Ashokkumar, Marwan Diapari, Ambuj B. Jha, Bunyamin Tar’an, Gene Arganosa, Thomas D. Warkentin. Genetic diversity of nutritionally important carotenoids in 94 pea and 121 chickpea accessions. Journal of Food Composition and Analysis 2015, 43 , 49-60. https://doi.org/10.1016/j.jfca.2015.04.014
- Ambuj B. Jha, Kaliyaperumal Ashokkumar, Marwan Diapari, Stephen J. Ambrose, Haixia Zhang, Bunyamin Tar’an, Kirstin E. Bett, Albert Vandenberg, Thomas D. Warkentin, Randall W. Purves. Genetic diversity of folate profiles in seeds of common bean, lentil, chickpea and pea. Journal of Food Composition and Analysis 2015, 42 , 134-140. https://doi.org/10.1016/j.jfca.2015.03.006
- Abhishek Bohra, Kanwar L. Sahrawat, Shiv Kumar, Rohit Joshi, Ashok K. Parihar, Ummed Singh, Deepak Singh, Narendra P. Singh. Genetics- and genomics-based interventions for nutritional enhancement of grain legume crops: status and outlook. Journal of Applied Genetics 2015, 56
(2)
, 151-161. https://doi.org/10.1007/s13353-014-0268-z
Article Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.
Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.
The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated.