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Blocking Peptides Decrease Tissue Transglutaminase Processing of Gliadin in Vitro

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Department of Chemical and Biological Engineering/Food Science, Chalmers University of Technology, SE-41296 Göteborg, Sweden
Department of Cell and Molecular Biology, Gothenburg University, SE-40530 Göteborg, Sweden
§ Department of Plant and Environmental Sciences, Gothenburg University, SE-40530 Göteborg, Sweden
*To whom correspondence should be addressed. Department of Chemical and Biological Engineering/Food Science, Chalmers University of Technology, SE-412 96, Göteborg, Sweden. Tel: +46 31-772-38-21. Fax: +46 31-772-3830. E-mail: [email protected]
∥Current address: Department of Pharmacology, Medical School, Academic Health Center, 55455 Minneapolis, MN.
Cite this: J. Agric. Food Chem. 2009, 57, 21, 10150–10155
Publication Date (Web):October 8, 2009
https://doi.org/10.1021/jf902268z
Copyright © 2009 American Chemical Society

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    Abstract

    Tissue transglutaminase (tTG) plays an important role in celiac disease pathology as it catalyzes deamidation and cross-linking of specific gluten peptides and converts them into potent epitopes recognized by intestinal T-cells. We investigated whether synthetic peptides with high affinity to gliadin could alter tTG activity on gliadin and whole gluten digest. The immobilized substrates were incubated with synthetic peptides identified by the phage display technique and a control peptide with no affinity to gliadin. Transglutaminase activity was measured with time resolved fluorescence. The mean tTG activity, compared to that of the control without the peptides, was reduced by 31, 33, and 36% for three selected gliadin-binding peptides, and 30% for the peptide pool (P ≤ 0.001−0.004) when gliadin was the substrate. Finally, substrate specificity experiments suggested that avenin was processed in a manner similar that used for gliadin during in vitro assays with tTG. The results showed that the blocking peptides efficiently reduced tTG processing of gliadin in vitro, and this strategy will be further investigated as an alternative therapy for celiac disease.

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    Cited By

    This article is cited by 7 publications.

    1. Laya Heydari, Rouha Kasra Kermanshahi, Sara Gharavi, Zahra Moosavi-Nejad. Characterization of the recombinant PepX peptidase from Lactobacillus fermentum and its effect on gliadin protein hydrolysis in vitro. Biologia 2023, 78 (2) , 565-577. https://doi.org/10.1007/s11756-022-01273-7
    2. Charlene B. Van Buiten, Ryan J. Elias. Gliadin Sequestration as a Novel Therapy for Celiac Disease: A Prospective Application for Polyphenols. International Journal of Molecular Sciences 2021, 22 (2) , 595. https://doi.org/10.3390/ijms22020595
    3. Jonas F Ludvigsson, Julio C Bai, Federico Biagi, Timothy R Card, Carolina Ciacci, Paul J Ciclitira, Peter H R Green, Marios Hadjivassiliou, Anne Holdoway, David A van Heel, Katri Kaukinen, Daniel A Leffler, Jonathan N Leonard, Knut E A Lundin, Norma McGough, Mike Davidson, Joseph A Murray, Gillian L Swift, Marjorie M Walker, Fabiana Zingone, David S Sanders, . Diagnosis and management of adult coeliac disease: guidelines from the British Society of Gastroenterology. Gut 2014, 63 (8) , 1210-1228. https://doi.org/10.1136/gutjnl-2013-306578
    4. Rekha Kushwaha, Christina M. Payne, A. Bruce Downie. Uses of Phage Display in Agriculture: A Review of Food-Related Protein-Protein Interactions Discovered by Biopanning over Diverse Baits. Computational and Mathematical Methods in Medicine 2013, 2013 , 1-12. https://doi.org/10.1155/2013/653759
    5. Tingsu Chen, Karolina Hoffmann, Sofia Östman, Ann-Sofie Sandberg, Olof Olsson. Identification of gliadin-binding peptides by phage display. BMC Biotechnology 2011, 11 (1) https://doi.org/10.1186/1472-6750-11-16
    6. Karolina Hoffmann, Nils‐Gunnar Carlsson, Marie Alminger, Tingsu Chen, Agnes Wold, Olof Olsson, Ann‐Sofie Sandberg. In vitro digestive stability of complexes between gliadin and synthetic blocking peptides. Biotechnology and Applied Biochemistry 2011, 58 (3) , 190-197. https://doi.org/10.1002/bab.27
    7. Ivana Caputo, Marilena Lepretti, Stefania Martucciello, Carla Esposito. Enzymatic Strategies to Detoxify Gluten: Implications for Celiac Disease. Enzyme Research 2010, 2010 , 1-9. https://doi.org/10.4061/2010/174354

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