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Flavonoids of Honey and Propolis:  Characterization and Effects on Hepatic Drug-Metabolizing Enzymes and Benzo[a]pyrene−DNA Binding in Rats

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Unité de Toxicologie Nutritionnelle, INRA, 17 rue Sully, 21034 Dijon Cedex, France, and Station de Technologie des Produits Végétaux, INRA-Domaine Saint Paul, 84143 Montfavet Cedex, France
Cite this: J. Agric. Food Chem. 1996, 44, 8, 2297–2301
Publication Date (Web):August 15, 1996
https://doi.org/10.1021/jf9504733
Copyright © 1996 American Chemical Society

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    Abstract

    The influence of dietary sunflower honey, propolis, and a flavonoid extract of propolis was examined on drug-metabolizing enzyme activities in rat liver and on microsome-mediated binding of benzo[a]pyrene to DNA. Characterization of flavonoids present in sunflower honey and propolis was achieved in order to assess the relative effects of different components of honey and propolis. Honey and propolis contained the same major flavonoids, pinocembrin, chrysin, galangin, and pinobanksin. The concentration of flavonoids was higher in propolis. Sunflower honey produced no significant changes on phase I and phase II enzyme activities and no modification of in vitro binding of benzo[a]pyrene to DNA. Propolis treatment produced an increase of ethoxyresorufin deethylase, pentoxyresorufin depentylase, ethoxycoumarin deethylase, glutathione transferase, and epoxide hydrolase activities. A flavonoid extract from propolis slightly enhanced only few enzyme activities, ethoxycoumarin deethylase and epoxide hydrolase. The induction pattern was similar to that observed with pinocembrin (a major flavonoid of propolis) administered solely. Binding of benzo[a]pyrene to DNA by microsomes from rats fed with propolis or a flavonoid extract from propolis was not significantly modified. These results contribute to identification of food or foodstuffs that can modify drug-metabolizing enzymes and binding of carcinogens to DNA.

    Keywords: Sunflower honey; propolis; flavonoids; drug-metabolizing enzymes; benzo[a]pyrene−DNA binding

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    *

     Author to whom correspondence should be addressed (e-mail [email protected]).

     Unité de Toxicologie Nutritionnelle.

     Station de Technologie des Produits Végétaux.

     Abstract published in Advance ACS Abstracts, July 15, 1996.

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