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Analysis of Hygrine and Cuscohygrine in Coca Leaves Using Gas Chromatography and High-Performance Liquid Chromatography
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    Analysis of Hygrine and Cuscohygrine in Coca Leaves Using Gas Chromatography and High-Performance Liquid Chromatography
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    Plant Sciences Institute, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705
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    Journal of Agricultural and Food Chemistry

    Cite this: J. Agric. Food Chem. 1997, 45, 8, 3114–3117
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    https://doi.org/10.1021/jf960967f
    Published August 18, 1997
    Copyright © 1997 American Chemical Society

    Abstract

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    The alkaloids hygrine and cuscohygrine in Erythroxylum coca var. Coca leaves were analyzed by a gas chromatographic (GC) method using a dimethylsilicone capillary column and a high-performance liquid chromatographic (HPLC) method using a weak cation exchange column. Hygrine content in E. coca leaves was determined as 0.12% by GC and 0.07% by HPLC, whereas cuscohygrine content was 0.25% by GC and 0.21% by HPLC.

    Keywords: Hygrine; cuscohygrine; alkaloids; Erythroxylum; coca extracts

    Copyright © 1997 American Chemical Society

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    This article is cited by 7 publications.

    1. Xiao Xia, Yan Lu, Dao-Feng Chen. 2-Acylpyrrole-based alkaloids from the leaves of Sauropus spatulifolius and their α-glucosidase inhibitory activities. Bioorganic Chemistry 2025, 155 , 108134. https://doi.org/10.1016/j.bioorg.2025.108134
    2. Sophia Laposchan, Ruben F. Kranenburg, Arian C. van Asten. Impurities, adulterants and cutting agents in cocaine as potential candidates for retrospective mining of GC-MS data. Science & Justice 2022, 62 (1) , 60-75. https://doi.org/10.1016/j.scijus.2021.11.004
    3. Nelida Cristina Rubio, Denise Thurmann, Franziska Krumbiegel, Fritz Pragst. Behaviour of hygrine and cuscohygrine in illicit cocaine production establishes their use as markers for chewing coca leaves in contrast with cocaine abuse. Drug Testing and Analysis 2017, 9 (2) , 323-326. https://doi.org/10.1002/dta.1972
    4. Nelida Cristina Rubio, Martin Hastedt, Jorge Gonzalez, Fritz Pragst. Possibilities for discrimination between chewing of coca leaves and abuse of cocaine by hair analysis including hygrine, cuscohygrine, cinnamoylcocaine and cocaine metabolite/cocaine ratios. International Journal of Legal Medicine 2015, 129 (1) , 69-84. https://doi.org/10.1007/s00414-014-1061-6
    5. C. Rubio, S. Strano-Rossi, M.J. Tabernero, L. Anzillotti, M. Chiarotti, A.M. Bermejo. Hygrine and cuscohygrine as possible markers to distinguish coca chewing from cocaine abuse in workplace drug testing. Forensic Science International 2013, 227 (1-3) , 60-63. https://doi.org/10.1016/j.forsciint.2012.09.005
    6. Stefan Biastoff, Birgit Dräger. Chapter 2 Calystegines. 2007, 49-102. https://doi.org/10.1016/S1099-4831(07)64002-4
    7. Birgit Dräger. Analysis of tropane and related alkaloids. Journal of Chromatography A 2002, 978 (1-2) , 1-35. https://doi.org/10.1016/S0021-9673(02)01387-0

    Journal of Agricultural and Food Chemistry

    Cite this: J. Agric. Food Chem. 1997, 45, 8, 3114–3117
    Click to copy citationCitation copied!
    https://doi.org/10.1021/jf960967f
    Published August 18, 1997
    Copyright © 1997 American Chemical Society

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