ACS Publications. Most Trusted. Most Cited. Most Read
My Activity

Figure 1Loading Img

Carotenoid Charge Transfer States and Their Role in Energy Transfer Processes in LH1–RC Complexes from Aerobic Anoxygenic Phototrophs

View Author Information
Faculty of Science, University of South Bohemia, Branišovská 31, 370 05 České Budějovice, Czech Republic
Department of Chemistry, University of Connecticut, 55 North Eagleville Road, Storrs, Connecticut 06269-3060, United States
§ Institute of Microbiology, Department of Phototrophic Microorganisms − Algatech, 379 81 Třeboň, Czech Republic
Biological Centre, Czech Academy of Sciences, Branišovská 31, 370 05 České Budějovice, Czech Republic
Cite this: J. Phys. Chem. B 2013, 117, 38, 10987–10999
Publication Date (Web):November 6, 2012
Copyright © 2012 American Chemical Society

    Article Views





    Other access options
    Supporting Info (1)»


    Abstract Image

    Light-harvesting complexes ensure necessary flow of excitation energy into photosynthetic reaction centers. In the present work, transient absorption measurements were performed on LH1–RC complexes isolated from two aerobic anoxygenic phototrophs (AAPs), Roseobacter sp. COL2P containing the carotenoid spheroidenone, and Erythrobacter sp. NAP1 which contains the carotenoids zeaxanthin and bacteriorubixanthinal. We show that the spectroscopic data from the LH1–RC complex of Roseobacter sp. COL2P are very similar to those previously reported for Rhodobacter sphaeroides, including the transient absorption spectrum originating from the intramolecular charge-transfer (ICT) state of spheroidenone. Although the ICT state is also populated in LH1–RC complexes of Erythrobacter sp. NAP1, its appearance is probably related to the polarity of the bacteriorubixanthinal environment rather than to the specific configuration of the carotenoid, which we hypothesize is responsible for populating the ICT state of spheroidenone in LH1–RC of Roseobacter sp. COL2P. The population of the ICT state enables efficient S1/ICT-to-bacteriochlorophyll (BChl) energy transfer which would otherwise be largely inhibited for spheroidenone and bacteriorubixanthinal due to their low energy S1 states. In addition, the triplet states of these carotenoids appear well-tuned for efficient quenching of singlet oxygen or BChl-a triplets, which is of vital importance for oxygen-dependent organisms such as AAPs.

    Read this article

    To access this article, please review the available access options below.

    Get instant access

    Purchase Access

    Read this article for 48 hours. Check out below using your ACS ID or as a guest.


    Access through Your Institution

    You may have access to this article through your institution.

    Your institution does not have access to this content. You can change your affiliated institution below.

    Supporting Information

    Jump To

    Chromatogram demonstrating purity of bacteriorubixanthinal, global fitting results of bacteriorubixanthinal in solution and of LH1–RC from Roseobacter sp. COL2P in carotenoid radical region, kinetics of carotenoid signals in the LH1–RC complexes vs in solution together with BChl-a bleaching signal, comparison of LH1–RC transient absorption spectra of Roseobacter sp. COL2P and Rba. sphaeroides, and transient absorption spectra of LH1–RC complexes from Roseobacter sp. COL2P and Erythrobacter sp. NAP1 after excitation at 866 nm. This material is available free of charge via the Internet at

    Terms & Conditions

    Most electronic Supporting Information files are available without a subscription to ACS Web Editions. Such files may be downloaded by article for research use (if there is a public use license linked to the relevant article, that license may permit other uses). Permission may be obtained from ACS for other uses through requests via the RightsLink permission system:

    Cited By

    This article is cited by 15 publications.

    1. Owen Thwaites, Bern M. Christianson, Alexander J. Cowan, Frank Jäckel, Lu-Ning Liu, Adrian M. Gardner. Unravelling the Roles of Integral Polypeptides in Excitation Energy Transfer of Photosynthetic RC-LH1 Supercomplexes. The Journal of Physical Chemistry B 2023, 127 (33) , 7283-7290.
    2. Gustavo Mondragón-Solórzano, Jacinto Sandoval-Lira, Jorge Nochebuena, G. Andrés Cisneros, Joaquín Barroso-Flores. Electronic Structure Effects Related to the Origin of the Remarkable Near-Infrared Absorption of Blastochloris viridis’ Light Harvesting 1-Reaction Center Complex. Journal of Chemical Theory and Computation 2022, 18 (7) , 4555-4564.
    3. Andrei Razjivin, Jan Götze, Evgeny Lukashev, Vladimir Kozlovsky, Aleksandr Ashikhmin, Zoya Makhneva, Andrey Moskalenko, Heiko Lokstein, Vladimir Paschenko. Lack of Excitation Energy Transfer from the Bacteriochlorophyll Soret Band to Carotenoids in Photosynthetic Complexes of Purple Bacteria. The Journal of Physical Chemistry B 2021, 125 (14) , 3538-3545.
    4. Gürkan Keşan, Milan Durchan, Josef Tichý, Babak Minofar, Valentyna Kuznetsova, Marcel Fuciman, Václav Šlouf, Cemal Parlak, and Tomáš Polívka . Different Response of Carbonyl Carotenoids to Solvent Proticity Helps To Estimate Structure of the Unknown Carotenoid from Chromera velia. The Journal of Physical Chemistry B 2015, 119 (39) , 12653-12663.
    5. Nao Yukihira, Chiasa Uragami, Kota Horiuchi, Daisuke Kosumi, Alastair T. Gardiner, Richard J. Cogdell, Hideki Hashimoto. Intramolecular charge-transfer enhances energy transfer efficiency in carotenoid-reconstituted light-harvesting 1 complex of purple photosynthetic bacteria. Communications Chemistry 2022, 5 (1)
    6. Hideki Hashimoto, Chiasa Uragami, Nao Yukihira, Kota Horiuchi, Richard J. Cogdell. Ultrafast laser spectroscopic studies on carotenoids in solution and on those bound to photosynthetic pigment-protein complexes. 2022, 1-51.
    7. Nupur, Marek Kuzma, Jan Hájek, Pavel Hrouzek, Alastair T. Gardiner, Martin Lukeš, Martin Moos, Petr Šimek, Michal Koblížek. Structure elucidation of the novel carotenoid gemmatoxanthin from the photosynthetic complex of Gemmatimonas phototrophica AP64. Scientific Reports 2021, 11 (1)
    8. Elliot J. Taffet, Gregory D. Scholes. The A g + state falls below 3 A g - at carotenoid-relevant conjugation lengths. Chemical Physics 2018, 515 , 757-767.
    9. Kasia Piwosz, David Kaftan, Jason Dean, Jiří Šetlík, Michal Koblížek. Nonlinear effect of irradiance on photoheterotrophic activity and growth of the aerobic anoxygenic phototrophic bacterium Dinoroseobacter shibae. Environmental Microbiology 2018, 20 (2) , 724-733.
    10. A. P. Razjivin, E. P. Lukashev, V. O. Kompanets, V. S. Kozlovsky, A. A. Ashikhmin, S. V. Chekalin, A. A. Moskalenko, V. Z. Paschenko. Excitation energy transfer from the bacteriochlorophyll Soret band to carotenoids in the LH2 light-harvesting complex from Ectothiorhodospira haloalkaliphila is negligible. Photosynthesis Research 2017, 133 (1-3) , 289-295.
    11. Dariusz M. Niedzwiedzki, Preston L. Dilbeck, Qun Tang, Elizabeth C. Martin, David F. Bocian, C. Neil Hunter, Dewey Holten. New insights into the photochemistry of carotenoid spheroidenone in light-harvesting complex 2 from the purple bacterium Rhodobacter sphaeroides. Photosynthesis Research 2017, 131 (3) , 291-304.
    12. Vladimir Yurkov, Elizabeth Hughes. Aerobic Anoxygenic Phototrophs: Four Decades of Mystery. 2017, 193-214.
    13. Vadim Selyanin, Dzmitry Hauruseu, Michal Koblížek. The variability of light-harvesting complexes in aerobic anoxygenic phototrophs. Photosynthesis Research 2016, 128 (1) , 35-43.
    14. Michal Koblížek, . Ecology of aerobic anoxygenic phototrophs in aquatic environments. FEMS Microbiology Reviews 2015, 39 (6) , 854-870.
    15. Yuki Sato-Takabe, Koji Hamasaki, Koji Suzuki. Photosynthetic Competence of the Marine Aerobic Anoxygenic Phototrophic Bacterium Roseobacter sp. under Organic Substrate Limitation. Microbes and Environments 2014, 29 (1) , 100-103.

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    You’ve supercharged your research process with ACS and Mendeley!

    STEP 1:
    Click to create an ACS ID

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Your Mendeley pairing has expired. Please reconnect