ACS Publications. Most Trusted. Most Cited. Most Read
Mechanism of Indium(III) Exchange between NTA and Transferrin: A Kinetic Approach
My Activity

Figure 1Loading Img
    Article

    Mechanism of Indium(III) Exchange between NTA and Transferrin: A Kinetic Approach
    Click to copy article linkArticle link copied!

    View Author Information
    Dipartimento di Chimica e Chimica Industriale, University of Pisa, Via Risorgimento 35, 56100 Pisa, Italy
    * To whom correspondence should be addressed. Telephone: +39-050-2219259. Fax: +39-050-2219260. E-mail: [email protected]
    Other Access OptionsSupporting Information (1)

    The Journal of Physical Chemistry B

    Cite this: J. Phys. Chem. B 2008, 112, 38, 12168–12173
    Click to copy citationCitation copied!
    https://doi.org/10.1021/jp8045033
    Published August 30, 2008
    Copyright © 2008 American Chemical Society

    Abstract

    Click to copy section linkSection link copied!

    The equilibria and kinetics for the process of In3+ exchange between nitrilotriacetic acid (NTA) and bovine serum transferrin (T) have been investigated in aqueous solution containing sodium bicarbonate. The metal exchange equilibria have been measured by difference ultraviolet spectroscopy at 25 °C, pH = 7.4, and I = 0.2 M (NaClO4). The acid dissociation constants of NTA and the binding constants of In(III) to NTA have also been measured. Kinetic experiments revealed that the process of In3+ uptake by transferrin from [In(NTA)2]3- is biphasic, the fast phase being completed in a few seconds, the slow phase lasting for hours. The fast phase has been investigated by the stopped-flow method and results in monoexponential kinetics. It involves rapid interaction of the 1:1 complex ML (M = In, L = NTA) with TB (T = transferrin, B = CO32−) to give a quaternary intermediate MLTB which then evolves to an “open” MTB* ternary complex complex with expulsion of L. In turn, this complex interconverts to a “closed”, more stable, form MTB. Neither the prevailing complex M2L nor the TB2 form of transferrin are directly involved in the exchange process but act as metal and protein reservoirs. The pH dependence of the reaction has been also investigated. The slow phase has not been investigated in detail; it takes several hours to go to the completeness, its slowness being ascribed to metal redistribution between the C-site and N-site of the protein, and/or metal release from polynuclear In(III) species.

    Copyright © 2008 American Chemical Society

    Read this article

    To access this article, please review the available access options below.

    Get instant access

    Purchase Access

    Read this article for 48 hours. Check out below using your ACS ID or as a guest.

    Recommended

    Access through Your Institution

    You may have access to this article through your institution.

    Your institution does not have access to this content. Add or change your institution or let them know you’d like them to include access.

    Supporting Information

    Click to copy section linkSection link copied!

    Figures showing potentiometric titration curves of NTA and In−NTA, species distribution plots for NTA, indium−NTA complexes, and indium−NTA−transferrin systems, and the difference spectra of transferrin in the presence of different amounts of [In(NTA)2]3−, text giving the mathematical derivation of eqs 3, 4, and 5 and a scheme showing the reactions used in the derivation of eq 5. This material is available free of charge via the Internet at http://pubs.acs.org.

    Terms & Conditions

    Most electronic Supporting Information files are available without a subscription to ACS Web Editions. Such files may be downloaded by article for research use (if there is a public use license linked to the relevant article, that license may permit other uses). Permission may be obtained from ACS for other uses through requests via the RightsLink permission system: http://pubs.acs.org/page/copyright/permissions.html.

    Cited By

    Click to copy section linkSection link copied!

    This article is cited by 9 publications.

    1. Shreya Pramanik, Jan Steinkühler, Rumiana Dimova, Joachim Spatz, Reinhard Lipowsky. Binding of His-tagged fluorophores to lipid bilayers of giant vesicles. Soft Matter 2022, 18 (34) , 6372-6383. https://doi.org/10.1039/D2SM00915C
    2. Concepción Avila‐Montiel, Hugo Tlahuext, Armando Ariza, Carolina Godoy‐Alcántar, Antonio R. Tapia‐Benavides, Dra. Margarita Tlahuextl. Indium Coordination Compounds Derived from Amino Amides. European Journal of Inorganic Chemistry 2022, 2022 (20) https://doi.org/10.1002/ejic.202200178
    3. Josep Galceran, Encarna Companys, Jaume Puy, Jose Paulo Pinheiro, Elise Rotureau. AGNES in irreversible systems: The indium case. Journal of Electroanalytical Chemistry 2021, 901 , 115750. https://doi.org/10.1016/j.jelechem.2021.115750
    4. Tarita Biver, Monia Kraiem, Fernando Secco, Marcella Venturini. On the mechanism of indium(III) complex formation with metallochromic indicators. Polyhedron 2018, 156 , 6-13. https://doi.org/10.1016/j.poly.2018.09.004
    5. Marjan H. Tehrani, Encarna Companys, Angela Dago, Jaume Puy, Josep Galceran. Free indium concentration determined with AGNES. Science of The Total Environment 2018, 612 , 269-275. https://doi.org/10.1016/j.scitotenv.2017.08.200
    6. Mengqun Yu, Zheguo Zhu, Hong Wang, Linyao Li, Fei Fu, Yang Song, Erqun Song. Antibiotics mediated facile one-pot synthesis of gold nanoclusters as fluorescent sensor for ferric ions. Biosensors and Bioelectronics 2017, 91 , 143-148. https://doi.org/10.1016/j.bios.2016.11.052
    7. Francois Taute, Martin Onani, Abram Madiehe, Mervin Meyer. Aqueous soluble gold nanoparticle synthesis using polyethyleneimine and reduced glutathione. International Journal of Materials Research 2014, 105 (10) , 1025-1039. https://doi.org/10.3139/146.111114
    8. John B. Vincent, Sharifa Love. The binding and transport of alternative metals by transferrin. Biochimica et Biophysica Acta (BBA) - General Subjects 2012, 1820 (3) , 362-378. https://doi.org/10.1016/j.bbagen.2011.07.003
    9. Penelope J. Brothers, Christy E. Ruggiero. Coordination and Solution Chemistry of the Metals: Biological, Medical and Environmental Relevance. 2011, 519-611. https://doi.org/10.1002/9780470976548.ch9

    The Journal of Physical Chemistry B

    Cite this: J. Phys. Chem. B 2008, 112, 38, 12168–12173
    Click to copy citationCitation copied!
    https://doi.org/10.1021/jp8045033
    Published August 30, 2008
    Copyright © 2008 American Chemical Society

    Article Views

    340

    Altmetric

    -

    Citations

    Learn about these metrics

    Article Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.

    Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.

    The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated.