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Measurement of the Interaction Forces between Proteins and Iniferter-Based Graft-Polymerized Surfaces with an Atomic Force Microscope in Aqueous Media

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Department of Bioengineering, National Cardiovascular Center Research Institute, Suita, Osaka 565-8565, Japan, and Department of Biomedical Engineering, Graduate School of Medicine, Kyushu University, Higashi-ku, Fukuoka 812-8582, Japan
Cite this: Langmuir 2001, 17, 4, 1080–1087
Publication Date (Web):January 3, 2001
https://doi.org/10.1021/la000003p
Copyright © 2001 American Chemical Society

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    Abstract

    To investigate the characteristics of interaction forces between proteins and end-grafted polymer surfaces, force-versus-distance curves (f−d curves) were measured between protein-fixed probe tips (albumin (Alb) and lysozyme (Lyso)) and surfaces graft-polymerized with N,N-dimethylacrylamide (DMAAm) or acrylic acid (AAc) in an aqueous solution, using an atomic force microscope. DMAAm graft-polymerized surfaces with different chain lengths and AAc graft-polymerized surface were prepared by photopolymerization on a dithiocarbamate (iniferter)-immobilized surface. The effects of grafted chain length, grafting density, and electrostatic property of the grafted chain segments on the interaction forces in the processes of protein adsorption onto and desorption from the graft-polymerized surfaces were analyzed from the approaching and retracting traces of the observed f−d curves, respectively. (1) In the Alb/poly(DMAAm) system, steric repulsion was observed, in which the interaction range and the compressive force of the poly(DMAAm) layer linearly increased with increasing chain length of poly(DMAAm) except for very short chain lengths. Adhesion force was observed only for the poly(DMAAm) layer with short chains. (2) In the Alb/poly(AAc) system, repulsive force due to steric and electrostatic interactions, and “tooth-like” adhesion forces were observed. (3) In the Lyso/poly(AAc) system, electrostatic attraction and adhesion forces were observed. From observation 1, the grafting density, the elastic modulus of the poly(DMAAm) layer, and the conformation of the grafted chain (“mushroom” or “brush”) were deduced and are discussed in relation to the characteristics of the interaction force with the proteins. From observations 2 and 3, it was found that a polyanionic surface can provide a significant adhesion force not only to positively charged proteins but also to negatively charged ones at physiological pH.

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     National Cardiovascular Center Research Institute.

     Present address:  Department of Physics, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan, and CREST, Japan Science and Technology Corp., Kawaguchi, Saitama 332-0012, Japan.

    *

     To whom correspondence should be addressed:  TEL:  +81-92-642-6210. FAX:  +81-92-642-6212. E-mail:  matsuda@ med.kyushu-u.ac.jp.

    §

     Kyushu University.

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    9. Peng Gong and, I. Szleifer. Interactions between Charged Surfaces and Functionalized Grafted Polymer Layers. Industrial & Engineering Chemistry Research 2006, 45 (16) , 5466-5476. https://doi.org/10.1021/ie0510977
    10. Jill E. Headrick and, Cindy L. Berrie. Alternative Method for Fabricating Chemically Functionalized AFM Tips:  Silane Modification of HF-Treated Si3N4 Probes. Langmuir 2004, 20 (10) , 4124-4131. https://doi.org/10.1021/la036425r
    11. Diane Goodman,, Jayachandran N. Kizhakkedathu, and, Donald E. Brooks. Attractive Bridging Interactions in Dense Polymer Brushes in Good Solvent Measured by Atomic Force Microscopy. Langmuir 2004, 20 (6) , 2333-2340. https://doi.org/10.1021/la035843t
    12. Yasuhide Nakayama,, Mika Sudo,, Kingo Uchida, and, Takehisa Matsuda. Spatio-Resolved Hyperbranched Graft Polymerized Surfaces by Iniferter-Based Photograft Copolymerization. Langmuir 2002, 18 (7) , 2601-2606. https://doi.org/10.1021/la011415g
    13. V. V. Tsukruk,, A. Sidorenko,, V. V. Gorbunov, and, S. A. Chizhik. Surface Nanomechanical Properties of Polymer Nanocomposite Layers. Langmuir 2001, 17 (21) , 6715-6719. https://doi.org/10.1021/la010761v
    14. Chen Wang, Hanying Zhao. Polymer brush-based nanostructures: from surface self-assembly to surface co-assembly. Soft Matter 2022, 18 (28) , 5138-5152. https://doi.org/10.1039/D2SM00458E
    15. Sayaka Masaike, Saori Sasaki, Hiroyuki Ebata, Kosuke Moriyama, Satoru Kidoaki. Adhesive-ligand-independent cell-shaping controlled by the lateral deformability of a condensed polymer matrix. Polymer Journal 2022, 54 (2) , 211-222. https://doi.org/10.1038/s41428-021-00577-w
    16. Kübra HÜKÜM ÖZKAN, Esma MUTLUTÜRK, Tugba DEMİR ÇALIŞKAN, Tuncer ÇAYKARA. Synthesis of Polymer Brushes by Surface-Initiated Controlled/Living Free Radical Polymerization Techniques. Hacettepe Journal of Biology and Chemistry 2020, 48 (5) , 395-405. https://doi.org/10.15671/hjbc.813565
    17. Juan M. Giussi, M. Lorena Cortez, Waldemar A. Marmisollé, Omar Azzaroni. Functionalization of Surfaces Using Polymer Brushes: An Overview of Techniques, Strategies, and Approaches. 2017, 1-27. https://doi.org/10.1002/9781119455042.ch1
    18. Gurvinder Singh, Kristen Bremmell, Hans J. Griesser, Peter Kingshott. Colloid-probe AFM studies of the surface functionality and adsorbed proteins on binary colloidal crystal layers. RSC Advances 2017, 7 (12) , 7329-7337. https://doi.org/10.1039/C6RA28491D
    19. Sho Sakata, Yuuki Inoue, Kazuhiko Ishihara. Precise control of surface electrostatic forces on polymer brush layers with opposite charges for resistance to protein adsorption. Biomaterials 2016, 105 , 102-108. https://doi.org/10.1016/j.biomaterials.2016.07.043
    20. Muhammad Khan, Noura Dosoky, John Williams. Engineering Lipid Bilayer Membranes for Protein Studies. International Journal of Molecular Sciences 2013, 14 (11) , 21561-21597. https://doi.org/10.3390/ijms141121561
    21. Mehmet Atilla Tasdelen, Yusuf Yagci. Controlled/Living Radical Polymerization in the Presence of Iniferters. 2013, 78-111. https://doi.org/10.1039/9781849737425-00078
    22. Rongmo Luo, Hua Li. Parameter Study of Glucose-Sensitive Hydrogel: Effect of Immobilized Glucose Oxidase on Diffusion and Deformation. Soft Materials 2013, 11 (1) , 69-74. https://doi.org/10.1080/1539445X.2011.580412
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    25. Joe-Ming Chang, Fan-Gang Tseng, Ching-Chang Chieng. Mixed-SAM Surfaces Monitoring CTX-Protein Part I: Using Atomic Force Microscope Measurements. IEEE Transactions on NanoBioscience 2010, 9 (4) , 289-296. https://doi.org/10.1109/TNB.2010.2070516
    26. Chi Yen, Hongyan He, Zhengzheng Fei, Xulang Zhang, L. James Lee, W. S. Winston Ho. Surface Modification of Nanoporous Poly(ϵ-caprolactone) Membrane with Poly(ethylene glycol) to Prevent Biofouling: Part II. Effects of Graft Density and Chain Length. International Journal of Polymeric Materials 2010, 59 (11) , 943-957. https://doi.org/10.1080/00914037.2010.504164
    27. Ali Ekrem Muftuoglu, Mehmet Atilla Tasdelen, Yusuf Yagci. Photografting of Polymeric Materials. 2010, 509-539. https://doi.org/10.1002/9780470594179.ch13
    28. Theodora S. Tsapikouni, Yannis F. Missirlis. Measuring the force of single protein molecule detachment from surfaces with AFM. Colloids and Surfaces B: Biointerfaces 2010, 75 (1) , 252-259. https://doi.org/10.1016/j.colsurfb.2009.08.041
    29. Rongmo Luo, Hua Li. Simulation analysis of effect of ionic strength on physiochemical and mechanical characteristics of glucose-sensitive hydrogels. Journal of Electroanalytical Chemistry 2009, 635 (2) , 83-92. https://doi.org/10.1016/j.jelechem.2009.08.009
    30. Hua Li, Rongmo Luo, Erik Birgersson, Khin Yong Lam. A chemo-electro-mechanical model for simulation of responsive deformation of glucose-sensitive hydrogels with the effect of enzyme catalysis. Journal of the Mechanics and Physics of Solids 2009, 57 (2) , 369-382. https://doi.org/10.1016/j.jmps.2008.10.007
    31. Rongmo Luo, Hua Li, Khin Yong Lam. Modeling the effect of environmental solution pH on the mechanical characteristics of glucose-sensitive hydrogels. Biomaterials 2009, 30 (4) , 690-700. https://doi.org/10.1016/j.biomaterials.2008.10.008
    32. Hua Li. Novel Models for Smart Hydrogel Responsive to Other Stimuli: Glucose Concentration and Ionic Strength. 2009, 295-333. https://doi.org/10.1007/978-3-642-02368-2_6
    33. Theodora S. Tsapikouni, Stephanie Allen, Yannis F. Missirlis. Measurement of interaction forces between fibrinogen coated probes and mica surface with the atomic force microscope: The pH and ionic strength effect. Biointerphases 2008, 3 (1) , 1-8. https://doi.org/10.1116/1.2840052
    34. Z Zhang, M R Tomlinson, R Golestanian, M Geoghegan. The interfacial behaviour of single poly( N , N -dimethylacrylamide) chains as a function of pH. Nanotechnology 2008, 19 (3) , 035505. https://doi.org/10.1088/0957-4484/19/03/035505
    35. , Stanislav Voronov, Volodymyr Samaryk, . Heterofunctional oligoperoxides on the interface. Chemistry & Chemical Technology 2007, 1 (1) , 1-13. https://doi.org/10.23939/chcht01.01.001
    36. George Greene, Harish Radhakrishna, Rina Tannenbaum. Protein binding properties of surface-modified porous polyethylene membranes. Biomaterials 2005, 26 (30) , 5972-5982. https://doi.org/10.1016/j.biomaterials.2005.03.025
    37. Tomoyuki Koga, Hideyuki Otsuka, Atsushi Takahara. Imaging of Charged Micropatterned Monolayer Surfaces by Chemical Force Microscopy. Bulletin of the Chemical Society of Japan 2005, 78 (9) , 1691-1698. https://doi.org/10.1246/bcsj.78.1691
    38. Yevgeny Moskovitz, Simcha Srebnik. Mean-Field Model of Immobilized Enzymes Embedded in a Grafted Polymer Layer. Biophysical Journal 2005, 89 (1) , 22-31. https://doi.org/10.1529/biophysj.104.053686
    39. Pankaj Vadgama. 2  Surface biocompatibility. Annual Reports Section "C" (Physical Chemistry) 2005, 101 , 14. https://doi.org/10.1039/b408906p
    40. Takehisa Matsuda, Masayoshi Kaneko, Soren Ge. Quasi-living surface graft polymerization with phosphorylcholine group(s) at the terminal end. Biomaterials 2003, 24 (24) , 4507-4515. https://doi.org/10.1016/S0142-9612(03)00349-1
    41. Wen Guang Liu, Fang Li, Xiao Dong Zhao, Kang De Yao, Qing Gang Liu. Atom force microscopic characterisation of the interaction forces between bovine serum albumin and cross‐linked alkylated chitosan membranes in media of different pH. Polymer International 2002, 51 (12) , 1459-1463. https://doi.org/10.1002/pi.1085
    42. Satoru Kidoaki, Takehisa Matsuda. Mechanistic aspects of protein/material interactions probed by atomic force microscopy. Colloids and Surfaces B: Biointerfaces 2002, 23 (2-3) , 153-163. https://doi.org/10.1016/S0927-7765(01)00232-6