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Molecular Imaging of a Micropatterned Biological Ligand on an Activated Polymer Surface

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    Research Article

    Molecular Imaging of a Micropatterned Biological Ligand on an Activated Polymer Surface
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    Department of Biomedical Engineering, Box 90281, Duke University, Durham, North Carolina 27708, Physical Electronics, 6509 Flying Cloud Drive, Eden Prairie, Minnesota 55344, and Becton Dickinson Research Center, Research Triangle Park, North Carolina 27709
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    Langmuir

    Cite this: Langmuir 2000, 16, 19, 7482–7492
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    https://doi.org/10.1021/la0000623
    Published August 18, 2000
    Copyright © 2000 American Chemical Society
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    Abstract

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    We report here molecular characterization of a new method derived from reactive microcontact printingmicrostamping on an activated polymer surface (MAPS)which enables biological ligands and proteins to be patterned on a polymer surface with a spatial resolution of at least 5 μm and good reproducibility. MAPS is a multistep procedure:  first, the surface of a polymer is modified, in one or more steps, to introduce a reactive group of interest. In a subsequent step, an elastomeric stamp, inked with a biological ligand containing a complementary terminal reactive group, is brought into contact with the activated surface of the polymer. This results in spatially resolved transfer and coupling of the biological ligand to the reactive surface of the polymer. We used MAPS to pattern biotin on carboxylic acid derivatized poly(ethylene terephthalate) (PET), and subsequently with streptavidin, mediated by the high affinity streptavidin−biotin interaction. X-ray photoelectron spectroscopy of biotin-derivatized PET showed that approximately one in five PET repeat units in the top 50−100 Å were functionalized with biotin. Time-of-flight secondary ion mass spectrometry (TOF-SIMS) suggested an increased concentration of PET oligomers in the top 10 Å due to chain scission during modification and clearly identified the derivatization of PET with biotin. TOF-SIMS imaging mapped biotin and streptavidin to the stamped regions. TOF-SIMS also imaged the spatial distribution of residual reagents from the multistep derivatization in MAPS, such as pentafluorophenol, Tween 20 surfactant, as well as poly(dimethylsiloxane) (PDMS), which was transferred from the elastomeric PDMS stamp to the surface during MAPS.

    Copyright © 2000 American Chemical Society

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    †

     Duke University.

    ‡

     Physical Electronics.

    §

     Becton Dickinson Research Center.

    *

     To whom correspondence should be addressed. Tel:  (919) 660-5373. FAX:  (919) 660-5362. E-mail:  [email protected].

    Cited By

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    This article is cited by 56 publications.

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    Langmuir

    Cite this: Langmuir 2000, 16, 19, 7482–7492
    Click to copy citationCitation copied!
    https://doi.org/10.1021/la0000623
    Published August 18, 2000
    Copyright © 2000 American Chemical Society
    Request reuse permissions

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