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Microcontact Printing for Creation of Patterned Lipid Bilayers on Tetraethylene Glycol Self-Assembled Monolayers

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Department of Chemistry and Center for Materials Innovation, Washington University in St. Louis, St. Louis, Missouri 63130, United States
E-mail: [email protected]. Telephone: 314-935-4695. Fax: 314-935-4481.
Cite this: Langmuir 2011, 27, 19, 12052–12057
Publication Date (Web):August 25, 2011
https://doi.org/10.1021/la201839w
Copyright © 2011 American Chemical Society
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Abstract

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Supported lipid bilayers (SLBs) formed on many different substrates have been widely used in the study of lipid bilayers. However, most SLBs suffer from inhomogeneities due to interactions between the lipid bilayer and the substrate. In order to avoid this problem, we have used microcontact printing to create patterned SLBs on top of ethylene-glycol-terminated self-assembled monolayers (SAMs). Glycol-terminated SAMs have previously been shown to resist absorbance of biomolecules including lipid vesicles. In our system, patterned lipid bilayer regions are separated by lipid monolayers, which form over the patterned hexadecanethiol portions of the surface. Furthermore, we demonstrate that α-hemolysin, a large transmembrane protein, inserts preferentially into the lipid bilayer regions of the substrate.

Cited By


This article is cited by 10 publications.

  1. Jason N. Belling, Kevin M. Cheung, Joshua A. Jackman, Tun Naw Sut, Matthew Allen, Jae Hyeon Park, Steven J. Jonas, Nam-Joon Cho, Paul S. Weiss. Lipid Bicelle Micropatterning Using Chemical Lift-Off Lithography. ACS Applied Materials & Interfaces 2020, 12 (11) , 13447-13455. https://doi.org/10.1021/acsami.9b20617
  2. Matthew K. Strulson and Joshua A. Maurer . Mechanistic Insight into Patterned Supported Lipid Bilayer Self-Assembly. Langmuir 2012, 28 (38) , 13652-13659. https://doi.org/10.1021/la300670a
  3. Maite Garcia-Hernando, Fernando Benito-Lopez, Lourdes Basabe-Desmonts. Advances in Microtechnology for Improved Cytotoxicity Assessment. Frontiers in Materials 2020, 7 https://doi.org/10.3389/fmats.2020.582030
  4. Lorena Redondo-Morata, Patricia Losada-Pérez, Marina Inés Giannotti. Lipid bilayers: Phase behavior and nanomechanics. 2020,,, 1-55. https://doi.org/10.1016/bs.ctm.2020.08.005
  5. Wen-Dong Liu, Bai Yang. Patterned surfaces for biological applications: A new platform using two dimensional structures as biomaterials. Chinese Chemical Letters 2017, 28 (4) , 675-690. https://doi.org/10.1016/j.cclet.2016.09.004
  6. Ju Hwan Kim, Eun Jeong Kim, Ki Hoon Kang, Chong Seung Yoon. Non-Enzymatic Sensing of Hydrogen Peroxide Using Directly Deposited Au Nanoparticles on Solid-Supported Phospholipid Film. Journal of The Electrochemical Society 2017, 164 (14) , B753-B757. https://doi.org/10.1149/2.0591714jes
  7. Jasper van Weerd, Marcel Karperien, Pascal Jonkheijm. Supported Lipid Bilayers for the Generation of Dynamic Cell-Material Interfaces. Advanced Healthcare Materials 2015, 4 (18) , 2743-2779. https://doi.org/10.1002/adhm.201500398
  8. Heesuk Kim, Keel Yong Lee, Soo Ryeon Ryu, Kwang-Hwan Jung, Tae Kyu Ahn, Yeonhee Lee, Oh-Sun Kwon, Sung-Jin Park, Kevin Kit Parker, Kwanwoo Shin. Charge-selective membrane protein patterning with proteoliposomes. RSC Advances 2015, 5 (7) , 5183-5191. https://doi.org/10.1039/C4RA12088D
  9. Yinli Li, Changjiang Zhu, Jichun Zhu, Hao Liang, Dong Chen, Huiling Zhao, Bo Liu. Nanomechanics of phospholipid LB film studied layer by layer with AFM. Chemistry Central Journal 2014, 8 (1) https://doi.org/10.1186/s13065-014-0071-2
  10. Xi Ke, Jilin Tang. A Simple Method for Fabricating Patterned Curvilinear Microstructures in Poly(dimethylsiloxane) by Selective Wetting. ChemPhysChem 2013, 14 (5) , 946-951. https://doi.org/10.1002/cphc.201200954

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