Increased Stability of Glycol-Terminated Self-Assembled Monolayers for Long-Term Patterned Cell Culture
Abstract

Self-assembled monolayers (SAMs) are widely used to confine proteins and cells to a pattern to study cellular processes and behavior. To fully explore some of these phenomena, it is necessary to control cell growth and confinement for several weeks. Here, we present a simple method by which protein and cellular confinement to a pattern can be maintained for more than 35 days. This represents a significant increase in pattern stability compared to previous monolayer systems and is achieved using an amide-linked glycol monomer on 50 Å titanium/100 Å gold-coated glass coverslips. In addition, this study provides insight into the method of SAM degradation and excludes interfacial mixing of the monomers and blooming of the adlayer as major mechanisms for SAM degradation.
Cited By
This article is cited by 21 publications.
- Ruby Krueger, Kevin D. Moeller. Capitalizing on Mediated Electrolyses for the Construction of Complex, Addressable Molecular Surfaces. The Journal of Organic Chemistry 2021, Article ASAP.
- Guanyue Su, Hongchi Yu, Jinyong Hong, Xiaoli Wang, Tang Feng, Jiang Wu, Hongmei Yin, Yang Shen, Xiaoheng Liu. Integrin-Induced Signal Event Contributes to Self-Assembled Monolayers on Au-Nanoparticle-Regulated Cancer Cell Migration and Invasion. ACS Biomaterials Science & Engineering 2019, 5 (4) , 1804-1821. https://doi.org/10.1021/acsbiomaterials.8b01648
- Haruthai Pansuwan, Boonsong Ditmangklo, Chotima Vilaivan, Banphot Jiangchareon, Porntip Pan-In, Supason Wanichwecharungruang, Tanapat Palaga, Thanesuan Nuanyai, Chaturong Suparpprom, and Tirayut Vilaivan . Hydrophilic and Cell-Penetrable Pyrrolidinyl Peptide Nucleic Acid via Post-synthetic Modification with Hydrophilic Side Chains. Bioconjugate Chemistry 2017, 28 (9) , 2284-2292. https://doi.org/10.1021/acs.bioconjchem.7b00308
- Ferhat Aydinoglu, Hirotaka Yamada, Ripon K. Dey, and Bo Cui . Grafted Polystyrene Monolayer Brush as Both Negative and Positive Tone Electron Beam Resist. Langmuir 2017, 33 (20) , 4981-4985. https://doi.org/10.1021/acs.langmuir.7b00412
- Matthew D. Graaf, Bernadette V. Marquez, Nai-Hua Yeh, Suzanne E. Lapi, and Kevin D. Moeller . New Methods for the Site-Selective Placement of Peptides on a Microelectrode Array: Probing VEGF–v107 Binding as Proof of Concept. ACS Chemical Biology 2016, 11 (10) , 2829-2837. https://doi.org/10.1021/acschembio.6b00685
- Matthew D. Graaf and Kevin D. Moeller . Introduction to Microelectrode Arrays, the Site-Selective Functionalization of Electrode Surfaces, and the Real-Time Detection of Binding Events. Langmuir 2015, 31 (28) , 7697-7706. https://doi.org/10.1021/la504254e
- Matthew J. Hynes and Joshua A. Maurer . Photoinduced Monolayer Patterning for the Creation of Complex Protein Patterns. Langmuir 2012, 28 (47) , 16237-16242. https://doi.org/10.1021/la303429a
- Yulia Zhukova, Christian Hiepen, Petra Knaus, Marc Osterland, Steffen Prohaska, John W. C. Dunlop, Peter Fratzl, Ekaterina V. Skorb. The Role of Titanium Surface Nanostructuring on Preosteoblast Morphology, Adhesion, and Migration. Advanced Healthcare Materials 2017, 6 (15) , 1601244. https://doi.org/10.1002/adhm.201601244
- Stephen F Bartolucci, Daniel Kaplan, Joshua A Maurer. Ion beam-induced hydroxylation controls molybdenum disulfide growth. 2D Materials 2017, 4 (2) , 021017. https://doi.org/10.1088/2053-1583/aa5e7e
- Nobuyuki Tanaka, Tadahiro Yamashita, Asako Sato, Viola Vogel, Yo Tanaka, . Simple agarose micro-confinement array and machine-learning-based classification for analyzing the patterned differentiation of mesenchymal stem cells. PLOS ONE 2017, 12 (4) , e0173647. https://doi.org/10.1371/journal.pone.0173647
- Bethany Almeida, Anita Shukla. Degradation of alkanethiol self-assembled monolayers in mesenchymal stem cell culture. Journal of Biomedical Materials Research Part A 2017, 105 (2) , 464-474. https://doi.org/10.1002/jbm.a.35922
- Myungwoong Kim, Samantha Schmitt, Jonathan Choi, John Krutty, Padma Gopalan. From Self-Assembled Monolayers to Coatings: Advances in the Synthesis and Nanobio Applications of Polymer Brushes. Polymers 2015, 7 (7) , 1346-1378. https://doi.org/10.3390/polym7071346
- Rosalynn Quiñones, Kate Rodriguez, Robbie J. Iuliucci. Investigation of phosphonic acid surface modifications on zinc oxide nanoparticles under ambient conditions. Thin Solid Films 2014, 565 , 155-164. https://doi.org/10.1016/j.tsf.2014.06.057
- Andrew E. Rodda, Laurence Meagher, David R. Nisbet, John S. Forsythe. Specific control of cell–material interactions: Targeting cell receptors using ligand-functionalized polymer substrates. Progress in Polymer Science 2014, 39 (7) , 1312-1347. https://doi.org/10.1016/j.progpolymsci.2013.11.006
- Yuekun Lai, Longxiang Lin, Fei Pan, Jianying Huang, Ran Song, Yongxia Huang, Changjian Lin, Harald Fuchs, Lifeng Chi. Bioinspired Patterning with Extreme Wettability Contrast on TiO 2 Nanotube Array Surface: A Versatile Platform for Biomedical Applications. Small 2013, 9 (17) , 2945-2953. https://doi.org/10.1002/smll.201300187
- Frank Meiners, Inka Plettenberg, Julia Witt, Britta Vaske, Andreas Lesch, Izabella Brand, Gunther Wittstock. Local control of protein binding and cell adhesion by patterned organic thin films. Analytical and Bioanalytical Chemistry 2013, 405 (11) , 3673-3691. https://doi.org/10.1007/s00216-013-6748-x
- Yuekun Lai, Fei Pan, Cong Xu, Harald Fuchs, Lifeng Chi. In Situ Surface-Modification-Induced Superhydrophobic Patterns with Reversible Wettability and Adhesion. Advanced Materials 2013, 25 (12) , 1682-1686. https://doi.org/10.1002/adma.201203797
- Matthew J. Hynes, Joshua A. Maurer. Lighting the path: photopatternable substrates for biological applications. Mol. BioSyst. 2013, 9 (4) , 559-564. https://doi.org/10.1039/C2MB25403D
- Samantha K. Schmitt, William L. Murphy, Padma Gopalan. Crosslinked PEG mats for peptide immobilization and stem cell adhesion. Journal of Materials Chemistry B 2013, 1 (9) , 1349. https://doi.org/10.1039/c2tb00253a
- Clifford A. Schlecht, Dawn M. Johnson, Joshua A. Maurer. Creation and control of defined protein patterns on glass: alkyl chain length controls stability. RSC Advances 2013, 3 (23) , 8826. https://doi.org/10.1039/c3ra21668c
- Dawn M. Johnson, Jad P. Abi-Mansour, Joshua A. Maurer. Spatial confinement instigates environmental determination of neuronal polarity. Integrative Biology 2012, 4 (9) , 1034-1037. https://doi.org/10.1039/c2ib20126g



