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Increased Stability of Glycol-Terminated Self-Assembled Monolayers for Long-Term Patterned Cell Culture

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Department of Chemistry and Center for Materials Innovation, Washington University in St. Louis, St. Louis, Missouri 63130, United States
*Telephone: 314-935-4695. Fax: 314-935-4481. E-mail: [email protected]
Cite this: Langmuir 2012, 28, 9, 4318–4324
Publication Date (Web):February 8, 2012
https://doi.org/10.1021/la2035533
Copyright © 2012 American Chemical Society
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Abstract

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Self-assembled monolayers (SAMs) are widely used to confine proteins and cells to a pattern to study cellular processes and behavior. To fully explore some of these phenomena, it is necessary to control cell growth and confinement for several weeks. Here, we present a simple method by which protein and cellular confinement to a pattern can be maintained for more than 35 days. This represents a significant increase in pattern stability compared to previous monolayer systems and is achieved using an amide-linked glycol monomer on 50 Å titanium/100 Å gold-coated glass coverslips. In addition, this study provides insight into the method of SAM degradation and excludes interfacial mixing of the monomers and blooming of the adlayer as major mechanisms for SAM degradation.

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Materials and instrumentation, synthesis of ester-linked glycol thiol (2), synthesis of amide-linked glycol thiol (3), polydimethylsiloxane (PDMS) stamp preparation, and patterned cell growth. This material is available free of charge via the Internet at http://pubs.acs.org.

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