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Photoinduced Monolayer Patterning for the Creation of Complex Protein Patterns

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Department of Chemistry and Center for Materials Innovation, Washington University in St. Louis, St. Louis, Missouri 63130, United States
Cite this: Langmuir 2012, 28, 47, 16237–16242
Publication Date (Web):November 12, 2012
https://doi.org/10.1021/la303429a
Copyright © 2012 American Chemical Society
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Abstract

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This work investigates self-assembled monolayers that were formed from a glycol-terminated thiol monomer and were patterned using photoinduced monolayer desorption. Utilizing direct-write photolithography provided a facile means to generate complex protein patterns containing gradients and punctate regions. The ablated glycol monolayers were characterized using scanning probe microscopy, which allowed us to observe differences in the nanomechanical properties between the patterned and nonpatterned regions of the substrate. The patterned regions on the surface adsorbed proteins, and this process was monitored quantitatively using surface plasmon resonance imaging (SPRi). Moreover, the concentration of the protein could be controlled accurately by simply setting the gray level in the 8-bit image. Adsorbed protein was probed using a commercially available antibody binding assay, which showed significant enhancement over the background. The ability to produce complex protein patterns will contribute greatly to creating in vitro models that more accurately mimic an in vivo environment.

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General experimental details, SPRi calibration, write-speed optimization, and full SPRi trace of the neutravidin binding assay. This material is available free of charge via the Internet at http://pubs.acs.org.

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Cited By


This article is cited by 2 publications.

  1. Sébastien G. Ricoult, Timothy E. Kennedy, David Juncker. Substrate-Bound Protein Gradients to Study Haptotaxis. Frontiers in Bioengineering and Biotechnology 2015, 3 https://doi.org/10.3389/fbioe.2015.00040
  2. Matthew J. Hynes, Joshua A. Maurer. Lighting the path: photopatternable substrates for biological applications. Mol. BioSyst. 2013, 9 (4) , 559-564. https://doi.org/10.1039/C2MB25403D

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