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High-Resolution Imaging of Chemical and Biological Sites on Living Cells Using Peak Force Tapping Atomic Force Microscopy

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Institute of Life Sciences and Institute of Condensed Matter and Nanosciences, Université Catholique de Louvain, Croix du Sud, 1, bte L7.04.01, B-1348 Louvain-la-Neuve, Belgium
Unité des Aspergillus, Institut Pasteur Paris France, 25 rue du docteur Roux, 75724 Paris, France
§ Fachbereich Biologie/Chemie, Universität Osnabrück, AG Genetik, Barbarastraße 11, D-49076 Osnabrück, Germany
*Phone: (32) 10 47 36 00. Fax: (32) 10 47 20 05. E-mail: [email protected]
Cite this: Langmuir 2012, 28, 49, 16738–16744
Publication Date (Web):November 30, 2012
https://doi.org/10.1021/la303891j
Copyright © 2012 American Chemical Society
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Abstract

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Currently, there is a growing need for methods that can quantify and map the molecular interactions of biological samples, both with high-force sensitivity and high spatial resolution. Force–volume imaging is a valuable atomic force microscopy (AFM) modality for probing specific sites on biosurfaces. However, the low speed and poor spatial resolution of this method have severely hampered its widespread use in life science research. We use a novel AFM mode (i.e., peak force tapping with chemically functionalized tips) to probe the localization and interactions of chemical and biological sites on living cells at high speed and high resolution (8 min for 1 μm × 1 μm images at 512 pixels × 512 pixels). First, we demonstrate the ability of the method to quantify and image hydrophobic forces on organic surfaces and on microbial pathogens. Next, we detect single sensor proteins on yeast cells, and we unravel their mechanical properties in relation to cellular function. Owing to its key capabilities (quantitative mapping, resolution of a few nanometers, and true correlation with topography), this novel biochemically sensitive imaging technique is a powerful complement to other advanced AFM modes for quantitative, high-resolution bioimaging.

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High-resolution images of single sensors on living yeast cells. This material is available free of charge via the Internet at http://pubs.acs.org.

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